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HYPOGLYCEMIC AND HYPOLIPIDEMIC EFFECTS OF SPIRULINA PLATENSIS, PHYCOCYANIN, PHYCOCYANOPEPTIDE AND PHYCOCYANOBILIN ON MALE DIABETIC RATS Full text
2018
El-Sayed El-Sayed | M. Hikal | B. Abo El- Khair | R. El-Ghobashy | A. El-Assar
This study aimed to evaluate hypoglycemic and hypolipidemic activities of Spirulina Platensis and its bioactive components (phycocyanin (PC), phycocyanopeptide (PCP) and phycocyanobilin (PCB)) on male diabetic Rats compared to controls and glibenclamide drug. For this reason, male Albino rats were equally divided into seven groups designated as normal control, diabetic control, diabetic + glibenclamide (Glyburide) drug (600 µg kg−1 body weight), diabetic + Spirulina biomass suspension (50 mg/ml/ kg−1 body weight), diabetic + phycocyanin (50 mg kg−1 body weight), diabetic + phycocyanopeptide (49 mg kg−1 body weight) and diabetic + phycocyanobilin (982 µg kg−1 body weight). The results show a statistically significant reduction (P < 0.05) level of fasting blood glucose, insulin resistance and lipids levels in diabetic animals administration with Spirulina Platensis, phycocyanin, phycocyanopeptide and phycocyanobilin compared with diabetic control. Also, there were an increase in HDL–cholesterol levels and β-cell function in these treatments. Histopathologically, diabetic rats treated with spirulina, PC, PCP induced a slight improve of pancreatic cells and an obvious recovery of pancreatic cells. The expression of insulin secretion from cells (β-cells) of diabetic rats was improved in the groups treated with Spirulina, phycocyanin, phycocyanopeptide. While, diabetic rats treated with phycocyanobilin recorded insulin levels lower than them. From this study it can be concluded that Spirulina Platensis, phycocyanin, phycocyanopeptide and phycocyanobilin possessed hypoglycemic, insulin sensitivity and hypolipidemic effects. Hypoglycemic and hypolipidemic effects of Spirulina Platensis may be attributed to phenolic compounds and phycocyanin. The antidiabetic effect of PC is most likely due to its ability to reduction of insulin resistance, enhance β-cell function and recovery of β-cells. The effect of PC may be attributed to selenium-binding phycocyanopeptide or/ and phycocyanobilin responsible for the antioxidant activity and chromium-binding phycocyanopeptide which activates insulin receptors.
Show more [+] Less [-]IDENTIFICATION AND CHARACTERIZATION OF HEAT SHOCK PROTEINS IN FOUR EGYPTIAN MAIZE INBRED LINES (Zea mays L.) Full text
2018
Fatma Mahmoud | M. Rashed | K. Khalil | M. Abou-Deif
The heat shock protein (HSP) family is one of the proteins universally accumulated under heat stress condition. Four Egyptian maize inbred lines (Zea mays L.) were studied to identify heat shock proteins under heat stress at 45°C as protein markers for detecting the thermo-tolerance inbreds. The protein fingerprints of four inbred lines were performed by grain total-soluble protein electcophoretic analysis. The protein electrophoretic analysis showed 18 bands in a distinct pattern of K1 and K7 inbred lines, while 17 bands were present in G342 and Rg59 inbred lines as another distinct pattern indicating different genotypes. The high temperature effect on four maize inbred lines exposed to 45°C for 2 and 4 hours at 14-days old seedlings besides control (25°C) was studied. Several protein bands varied between low and intermediate molecular weights were induced after exposing to heat stress at 45°C. Four bands of heat shock proteins with molecular weights of 82, 22, 17 and 10 kDa appeared in the inbred line K1 after exposing to 45°C for 2 and 4 hours which may be indication of thermo-tolerance. Four and seven bands were enhanced after exposing to high temperature for 4 hours at 45°C which appeared more concentrated in the patterns of K7 and G342 inbred lines, respectively.
Show more [+] Less [-]THE IMPACT OF THE PROBIOTIC BACTERIA ON THE CONTAMINATED MICROBIAL OF THE FEMALE GENITAL TRACT AND ITS EFFECT ON THE IVF OUTCOME Full text
2018
Hagar Ali | K. El-Dougdoug | M. .Soliman | M. Hazaa | M. M.M. Sowelam
The microbial contamination of the female genital tract has effect on the ivf outcome and negatively effect on fertility, fertilization and implantation rate. Lactobacillus sp. is the dominant organism of the vaginal flora that have an important role in the inhibition of pathogen. 48 patients were selected for this study aged between 20-40 years, During embryo transfer, 3 samples were collected from each patient from vagina, end cervical canal and the tip of the catheter. The samples were assayed microbiology. The microbial culture contained natural flora and pathogen organism .The isolated pathogens were identified as Klebsilla pneumonia, Escherichia coli, Candida albicans, C. tropicalis, C. famata, C. parapsilosis, C. catenulate according to Bergeyˊs manual and confirmed by VITEK System. Candida sp found to be the most frequent infemale genital tract. The probiotic (Lactobacillus. acidophilus) and its metabolites (bacteriocin) were inhibited growth of isolated pathogenic microbial in vitro.The result showed that L. acidophilus and their metabolite bacteriocin have antagonistic activity against pathogenic microbes. Recommendation: Oral or transvaginal administration of Lactobacillus or bacteriocin antiseptics; disinfected; maintain a health vagina and prevent the infection of the reproductive tract.
Show more [+] Less [-]GINGER ETHANOLIC EXTRACT, GINGER OIL OR RICE BRAN OIL INDUCED HEPATOPROTECTIVE EFFECT AGAINST FATTY LIVER IN RATS Full text
2018
H. Abd Allah | B. Abd El-wahab | K. Ramadan | S. Ali
Hepatoprotective effect of ethanol extract of ginger, ginger oil or rice bran oil against fatty liver disease which induced by ethanol stress was investigated in the present study. Thirty six (36) male albino rats were classified into 6 groups as follows: 1- Normal control (NC), 2- Positive control (induced fatty liver by ethanolic stress) (PC+), 3- rats group administered ethanol and ginger extract (GE group), 4- rats group administered ethanol and ginger oil (GO group), 5- rats group administered ethanol and rice bran oil (RBO group) and 6- rats group administered ethanol and DMSO (DMSO control group, because GE, GO and RBO were dissolved in DMSO as a vehicle). Results revealed that hepatic triglycerides was significantly (p≤0.05) raised to 80.7 mg/g liver, in positive control (PC+), compared to 15.98 mg/g liver in normal control (NC). Also significant increase (p≤0.05) in levels of ALT (69.41 U/L), AST (62.98 U/L) and ALP (121.65 U/L) in PC+, compared to their levels in NC (23.35 U/L), (27.95 U/L) and (73.45 U/L) respectively. In addition, high significant level was observed in serum triglycerides (214.37 mg/dl), total cholesterol (TC) (99.81 mg/dl) and LDL cholesterol (47.75 mg/dl) in PC+, compared with its values in NC group: (74.22 mg/dl), (31.45 mg/dl), (4.21 mg/dl) respectively. However, significant (p≤0.05) decrease was noticed in HDL cholesterol level (9.18 mg/dl) in PC+, compared to NC (12.39 mg/dl). On the other hand, treatment by ethanolic ginger extract (200 mg/kg body weight) showed a hepatoprotective effect which confirmed by remediation the values of hepatic TG, ALT, AST, ALP, TP, Alb, besides serum TG, TC, HDL-C and LDL-C in GE group as compared with their values in NC and PC+. Moreover, treatment by ginger oil (200 mg/kg body weight) and rice bran oil (200 mg/kg body weight) displayed a protective effect in GO or RBO groups, but lower than GE. In addition, ethanol extract of ginger disclosed very high antioxidant activity (IC50 = 18.25 µg/ml) compared to both ginger oil (IC50 = 6714.38 µg/ml) or rice bran oil (IC50 = 1409.57 µg/ml). Finally the present study indicates that ethanol extract of ginger showed hepatoprotective effect more than either ginger oil or rice bran oil.
Show more [+] Less [-]EXOGENOUS APPLIED PUTRESCINE ELEVATE DROUGHT TOLERANCE OF SUNFLOWER PLANTS BY MODIFYING OF SOME PHYSIO-BIOCHEMICAL PARAMETERS Full text
2018
A. Faisal | M. Ibrahim
Field Experiments were conducted at the Experimental Farm, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Egypt during 2014 and 2015 seasons to evaluate the effect of foliar spray of putrescine (Put) at 0.1 and 0.2 mM on growth and some physio-biochemical compounds of drought stressed sunflower. Putrescine application could be mitigate the negative effect of drought by increasing leaf relative water content (LRWC), proline, total soluble sugars, K and Ca concentrations of sunflower leaves. Correlation coefficient revealed that LRWC was inversely proportional to proline concentration on the one hand and directly proportional to K+ and Ca++ concentrations on the other hand. The modification of physio-biochemical parameters by Put treatments led to increase plant growth and seed-oil yield of sunflower under drought stress.
Show more [+] Less [-]COMPUTATIONAL MODEL TO IMPROVE DAIRY ANIMAL FEEDING UNDER MIXED FARMING SYSTEM (CROPS/LIVESTOCK) AS STUDY CASE Full text
2018
H. Ismail | A. Al-Sadek | A. Ashmawy | M. Khalil | Manal Elsayed
Computational model was designed for feeding systems of small dairy farms in Egypt under Mixed Farming System (MFS) (Crops/livestock). The present case study was selected from El-Beheira governorate, where the three common dairy animals (Local cows, Crossbred cows and buffaloes) are available. The main objectives of this study were 1- To find out the optimum combination of inputs from farm green forage and cash crops to minimize animal feeding costs. 2- Asses the possibilities of increasing the farm income by least cost rations formulation using available feed resources for dairy cattle. Technical coefficients of the models were obtained from previous studies under Egyptian condition. The model proposed three scenarios: Scenario I (S I) calculated the actual feeding situation from the case study without any changes as base run, scenario II (S II) proposed to cover animal feeding requirements of the same herd in scenario (S I) from the same available feed resources according to NRC (2001) and scenario III (S III) operating on the available feeding package quantities or reallocated farm feed resources for the same herd. The model used the common feed in summer and winter seasons (300 days) while, two months were considered as transitional period between two seasons, where irregular animal feeding regime is adopted. The results showed that area cultivated with green forages can be reduced by 17% and 25% of total planted area in SII for winter and summer, respectively, compared to base run (SI). Where as in S III, the green forage cultivated areas reduced by 30% and 25% for winter and summer, respectively in comparison with SI, feeding costs in SII were reduced by 51.11% and 38.97% in winter and summer, respectively. Using available feeding packages and reallocated farm resources in SIII reduced feeding costs by 47.78% and 27.67% for winter and summer, respectively. It can be concluded that using available feeding packages or reallocated animal feeding resources either in SII and SIII achieved a considerable reduction on animal feeding costs of small-scale mixed farms compared to base run scenario (SI).
Show more [+] Less [-]EFFECT OF BARLEY FLOUR ON WHEAT BREAD QUALITY Full text
2018
H. El-Taib | I. Rizk | E. Yousif | Amal Hassan
This study was carried out to investigate the possibility of utilization of barley flour in production of pan bread. wheat flour of (72% extract) was replaced by 10, 15 and 20% barley flour. The effect of such replacements on physical and chemical characteristics of produced pan bread were studied. The results indicated that barley flour had a higher contents of chemical composition than that in wheat flour except carbohydrate content was the highest in wheat flour (85.53%) than in barley flour (76.67%),whereas, protein, ash, dietary fiber, β-glucan and antioxidant were higher content in barley flour (13.63, 2.44, 19.00, 8.65, 97.34 respectively) as compared with its content in wheat flour (12.26, 0.49, 4.88, 0.34, 55.33 respctively),while, wet gluten and falling number were reduced by added barley in composite flour. The rheological properties of the five dough mixes were studied using farinograph and extensograph. The water absorption and dough weakening increased as the percentage of barley flour increased, while were decreased the extensibility and maximum resistance to extension . The results of sensory characteristies of prepared bread (containes10% barley flour) was not significants different from control for crust color, grain and texture. The results revealed that it was possible to use barley flour at level of 10, 15% to produce bread that satisfied baker's and consumer's sensory expectation. This levels may be to increased the nutritional value from dietary fibers, β-glucan, minerals, vitamins and antioxidants.
Show more [+] Less [-]ECONOMIC STUDY FOR THE SUPPLY RESPONSE OF SUGAR CROPS IN EGYPT Full text
2018
Eman Ahmed
Agriculture sector is one of essential sector in the Egyptian economy. The sector is the main source of food required by Egyptian people, in addition to that the sector is also a source of row materials for many in industries. On the other hands, sugar cane and sugar beet crops are the main source of sugar in Egypt as well as in many other countries. The average sugar cane area and production were about 0.32 millon Fadden and 15.60 millon tons during the period (1995-2014). While sugar beet area was 0.21 millon fadden and 4.53 millon ton during the years (1995-2014). The research problem may be concentrated on that domestic sugar production is short meeting local consumption and there for the government has to fill the gap through imports which means a burden on government budget. The shortage of domestic sugar production is mainly due to the limited area of sugar cane crop, which is mainly due to the limited agricultural land and water. The increase of population and the per capita consumption of sugar and the need to rationalize water use have made sugar production in Egypt more accurate. The main objective of the present research is therefore a trial to estimate farmers supply response of sugar response of sugar produced from sugar cane and sugar beet in an attempt to reduce sugar imports. The study has also tried to examine factors that may affect sugar consumption, i.e. social and political factors are also taken into consideration. The study showed that Brazil, India and China are the main producer of sugar cane, 48.32%, 29.32% and 9.31% respectively. The three countries produce together about 87% of sugar from sugar cane. On the other hand, France, USE, Germany produce together about 66% of total sugar beet. A study of supply response of sugar cane showed that farm price of sugar cane with two years lag and net return per Fadden with one year lag the important factors affective sugar cane production .As for sugar beet production, it was found that farm price and area with one year lag were the most important factor affecting sugar supply from sugar beet. The study recommends the expansion of sugar beet area particularly in the now reclaimed land in addition to of farming an encouraging price to farmers. Finally it is advisable to rationalize sugar consumption by Egyptian people.
Show more [+] Less [-]INFLUENCE OF IN-OVO INJECTION OF SELENIUM NANOPARTICLES AND SELENOMETHIONINE ON GROWTH PERFORMANCE AND PHYSIOLOGICAL PARAMETERS OF BROILER CHICKS Full text
2018
M. Abd El-Fatah | I. El-Wardany | E. Abdallah | Marwa Sh.
This study was conducted to investigate the effect of in ovo injection of broiler eggs with different levels and forms of selenium on growth performance and some blood parameters of post hatched chicks. A total of 300 fertilized eggs obtained from a commercial Arbor Acres broiler breeder flock, were used in the present experiment. They were divided randomly into six treatment groups, 50 eggs each. The first one was kept as a control– non injected group, the second group (sham) injected with phosphate buffered saline (PBS), the 3rd and 4th groups were injected with selenium nanoparticles (SeNPs) at 10 and 20 ug/egg, while the 5th and 6th groups were injected with organic selenium (Se-Methionine) at 50 and 100 ug/egg , respectively. All eggs were incubated in a forced draught incubator at the recommended temperature, relative humidity and turning patterns .The in ovo injection procedure was done at the 16th day of incubation. The criteria of response includes: live body weight, weight gain, feed consumption, feed conversion ratio, total protein, albumin, globulin, A/G ratio, cholesterol, triglyceride, HDL and glucose. The results showed that the supplementation of Se did not significantly affect growth performance traits. Supplementation of nano-Se increased plasma concentrations of total protein, albumin and HDL, and decrease glucose, however, no significant differences in globulin, A/G ratio, cholesterol and triglyceride levels. It can be concluded that the use of 20 ug /egg of Nano-Se caused an improvement of growth performance and feed conversion ratio without negative effects on blood constituents.
Show more [+] Less [-]OPTIMAL REQUIREMENTS OF NITROGEN AND PHOSPHORUS FERTILIZATION RATES FOR STRAWBERRY NURSERIES Full text
2018
A. Metwally | M. Ragab | S. El-Miniawy | S. Youssef
Due to the expansion of strawberry nursery cultivation, transplant production has become an important industry in Egypt. Nitrogen and phosphorus are the most important nutrients affecting the number and quality of strawberry transplants. However, no empirical research exists addressing the optimal requirements of nitrogen and phosphorus fertilization rates for strawberry nurseries in Egypt, until now. This study was conducted at a private farm in Abo Ghalib, Giza Governorate, Egypt during the two successive summer seasons of 2015 and 2016 to determine the optimal requirements of nitrogen and phosphorus fertilizations for Festival strawberry cultivar nurseries via evaluating the influence of four nitrogen rates (83, 100, 117 or 134 kg N/feddan) and five phosphorus rates (38, 62, 74, 86 or 98 kg P2O5/feddan) in a factorial experiment. Results revealed that increasing the rates of nitrogen and phosphorus fertilizations increased the number of main runners/mother plant and number of marketable transplants/m2, and enhanced all vegetative growth parameters, leaf SPAD readings, leaf relative water content, crown diameter, crown carbohydrates, and leaf mineral content in both growing seasons. On the contrary, both elements had an inverse effect on leaf total soluble phenols. Thus, the study recommends to the nurserymen of Festival straw-berry cultivar to apply 117 or 134 kg N with 98 kg P2O5 per feddan under pure sandy soil condition which gave the highest number of transplants with high quality and subsequent high quality crop in the field.
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