The aim of this study was to in vitro evaluate encapsulated probiotic supplementation to ruminant rationson degradation and fermentation parameters. The ration consisted of 40% alfalfa hay and 60% concentrate feed mixture. Encapsulated and not encapsulated probiotic were supplemented with level of 106cfu/kg of the total dry matter of ration (DM) and compared with encapsulation media (Sodium Alginate, SA) and control (not supplemented ration). DM and OM degradation and total gas production as well as fermentation parameters of the incubated samples were determined after 24 h of fermentation. Significant (P<0.01) increases in in-vitro DM degradability was observed for the experimental ration supplemented with encapsulated or not encapsulated probiotics at levels (106 CFU/ kg DM) and SA treatment compared to control ration. Also, significant (P<0.05) improvement in OM degradability was recorded for the ration supplemented with not encapsulated probiotics bacteria compared to the other treatments. Moreover no significant differences were observed between the control ration and the rations supplemented with encapsulated probiotics or SA only, as well as no significant difference was recorded between the ration supplemented with encapsulated probiotics and the ration supplemented with SA only. Probiotics bacteria supple mentation in the form of not encapsulated probiotic resulted significant increases in in vitro total gas production per sample and per g DM, OM, dDM, NDF and ADF after 24 hours incubation period compared to the other experimental rations (control, encapsulated probiotic and SA). While significant increase in total gas production per g dOM was observed for not encapsulated probiotic compared to encapsulated probiotic only. It could be concluded that, using encapsulated probiotics bacteria had no significant effect on DM degradability and may be induce decrease in gas production and fermentation parameters.

In the present study, two molecular biology tools based on DNA were compared in the differentiating between some microbial strains isolated from soil. Two types (16SrRNA and 18SrRNA) of ribosomal RNA genes were used for identification of the four bacterial and three fungal isolates, respectively. The identified microbial isolates were submitted in GenBank as strains of Escherichia coli MSL-19 (LC455952.1); Bacillus sp. MSLB-1 (LC455953.1); Bacillus sp. MSLB2 (LC455954.1); Bacillus sp. MSLB3 (LC455955.1); Penicillium sp. MLSP1 (LC455956.1); Aspergillus niger MLSAs1 (LC455958.1); Aspergillus sp. MLSAs2 (LC455959.1). The DNA obtained from the seven microbial strains was used as templates for RAPDPCR differentiating in the presence of eight random primers. Electrophoresis analysis was performed, and on scoring, the identity percentages between the bacterial and fungal strains were separately analyzed. A percentage of 82-83% was recorded between the E. coli and the three Bacillus strains, while, identities of 93-98% were recorded between the three Bacillus strains. Similar trend (90-96%) was observed between the Penicillium and Aspergillus strains. Results confirmed that identities based on the two ribosomal RNA genes (82-98%) was higher than that of RAPD-PCR (70.0-79.7%), and this is because of ribosomal RNA genes are in limited sizes (~1500-1600 bp) and specific for differentiating species, while RAPD-PCR tool depends on using some random primers could be recorded on the whole genome. The phylogenetic trees based on the two molecular  tools supported the obtained results. As a conclusion, tools of RAPD-PCR and ribosomal RNA genes were successfully used to identify and detect the genetic variability of microbial strains isolated from soil.

In the present study, a trail to evaluate of Newcastle Disease (ND) antibodies levels after different vaccination programs was conducted on layer chickens. A total of 200 one day-old layer chicks (White Lohmann) were divided into five groups A, B, C, D and E. Birds in groups A, B and C were vaccinated with live vaccine by intraocular, intranasal and drinking water methods , respectively. On the other hand, groups D and E were kept as unvaccinated control groups. Vaccination performed at days 5, 18 and 28 by different routes for mentioned groups. Hemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) methods were used for assessment of antibodies titer at days 15, 25, 37 and 45. Results of HI and ELISA tests indicated that, the intranasal and the intraocular method have highest antibodies titers compared with the drinking water method. In this study, maternally derived antibodies specific to Newcastle Disease Virus (NDV) (IgY) were extracted by dextran sulfate method from collected eggs of vaccinated laying chickens . Antibodies specific to NDV (IgY) were detected in the egg yolk using HI test  . Data revealed that antibodies specific to NDV (IgY) were presented in high titers that confer protection during early weeks of life for hatching chicks. Data concluded that extraction of maternally derived specific antibodies from egg yolk will facilitate accurate monitoring of ND vaccination programmes.

Forty-eight lactating Holstein Friesian cows were utilized to assess impact of supplementing probiotic ZAD (mixture of live bacterial cells and enzymes) compared with T5X (antitoxins product) on some productive, reproductive and antitoxins activity parameters. we have divided the animals to three experimental categories (16 each). The first category, control treatment, was fed basal diet without any supplements. The second category, ZAD treatment, was fed basal diet supplemented with ZAD probiotic (1.1 L/ton). The third category, T5X treatment, was fed basal diet supplemented with T5X antitoxins product (1.1 Kg/ton). Milk yield was recorded. we have collected and analyzed milk samples, blood samples and feed samples in order to constitutes. Time-interval between calving and fertilizing artificial insemination and pregnancy rate were recorded. The gained outcomes demonstrated that milk yield was significantly raised by ZAD treatment than other treatment. Milk components yield were significantly raised by ZAD probiotic and T5X treatments than control. Blood serum total protein, albumin, globulin, glucose, urea and total lipids were not significantly influenced by treatments. Total aflatoxins in concentrate mixture and aflatoxin M1 in milk were significantly diminished by ZAD and T5X than control. Number of days between calving and fertilized artificial insemination were diminished by treatments than control. Pregnancy rate was significantly raised by ZAD  probiotic than T5X and control treatments, respectively. The overall conclusion of this study illustrated that Probiotic ZAD, potentially, has antitoxins activity leading to reinforcing the productive and reproductive performance of lactating cows.

The experiment was held at the Arid land Agricultural graduate studies and Research Institute - Ain Shams University. A soil moisture sensor is designed and used for measuring and controlling soil moisture content in plant media.  The device has been programmed and connected to three water-lifting pumps to operate them according to each treatment.         A vertical system has been constructed using 18 columns of white square styrofoam pots filled with perlite (In-organic substrate). Three soil moisture content treatments were selected as T1 = 60 %, T2= 80%, and T3 = 100% which were controlled by the sensors.  Solution tank filled with dissolved nutrient elements was used for supplying required water.       Two types of lettuce (green and red) lettuce seedlings were planted in the perlite substrate and irrigated by drip irrigation system. The system was closed and was based on smart automatic drip irrigation system,        Results revealed that, 556.5 liters of water was consumed under condition of T1 = 60 %, while 697.5 liters and 908.5 were consumed under condition of T2= 80 %, and T3 = 100% respectively After 47 days the Red lettuce yield (weight, number of leaves, and also weight of dry lettuce) was higher than green lettuce for all treatments. Yields of lettuce per system unit (4.5 m2) 16 kg/m2 and water use efficiency 26kg /m3 (Barbosa et al 2015) conventional production yielded 3.9 ±  0.21 kg/m2/y of lettuce produce, with water. Hydroponics offered 11±1.7 times higher yields but required 82±11 times more energy compared to conventionally produced lettuce.

The antiviral role of Moringaoleifera leaves extracts ((chloroform (CL), Ethyl acetate (E.A), methanol 80% (M) and n.butanol (n.b) to control Coxsackie B (COX-BV) viral infection was monitored in vitro compared with human interferon alpha (IFNᾰ2a) in order to evaluate the antiviral activity of moringa leaves extracts. The phenolic compound was given higher conc. in E.A. extracts. Cytotoxicity was determined for Moringa leaves extracts on viability of HEP2 cell using MTT assay. The 98μg/ml concentrations for four extracts were considerably non-toxic for HEP2 cell line culture. Antiviral activity in Moringa extracts may be attributed to phenolic compounds and / or as a result of stimulation of COX-BV-sensitive HEP2 cells to express MX protein. Antiviral activity of Moringa leaves extracts compared with IFNᾰ-2a to cell lines against COX-BV viral infection was determined by assessment of the COX-BV virus infectivity titer declining rate and relative residual living cell count using End Point Assay, using three ways pretreatment, co-treatment and post-treatment cell line treated with Moringaextracts. It was found that the extract acetyl acetate was better as an MX gene expression level compared with its level in control cells. It was concluded that Moringaleaves extracts have antiviral activity against COX-BV.

The study was carried out  at  Faculty of Agriculture, Ain Shams University to assess the effect of two of algae species (Arthrospira platensis and Nannochloropsis gaditana) on growth performance of monosex  tilapia fish (Oreochromis niloticus), fish were fed on a basal diet supplemented with 0,3,5,7% of each algae. To perform seven experimental treatments were assigned in three replicates each. The experiment was designated as follows:  (T1) control (without algae), (T2) basal diet supplemented with 3% spirulina (spiru 3), (T3) basal diet supplemented with 5% spirulina (spiru 5), (T4) basal diet supplemented with 7% spirulina (spiru 7), (T5) basal diet supplemented with 3% Nannochloropsis (nanno 3), (T6) basal diet supplemented with 5% Nannochloropsis (nanno 5) and (T7) basal diet supplemented with 7% Nannochloropsis  (nanno 7). Experimental tanks were a part of closed recirculating system, where almost constant environmental conditions were kept throughout the experimental period. Twenty-one quadrate fiber glass tanks with 108-liter water capacity were stocked with 15 fish per tank. The individual initial body weight (2.7 g) was recorded at the beginning of the experiment. All fish in each tank was weighed every two weeks during the whole experimental period. The fish were fed the experimental diet for 95 days Results indicated that nanno 5 treatment had higher significant trend (P<0.05) final body weight (FBW), average weight  gain (AWG) and average daily gain (ADG) compared with the other experimental treatments. The best specific growth rate (SGR), feed conversion ratio (FCR) and feed efficiency ratio (FER) were found in nanno 7. Also, it was observed that spiru 7 have a high significant difference (P<0.05) in protein efficiency ratio (PER) and protein productive value (PPV). On the other hand, the results showed that the lowest AWG, ADG and (FER) appeared in spiru 7%. No mortality in nanno7 and spiru 7. Finally, using of nano 5% or spiru 7% enhance growth performance and feeding in tilapia fish.

This investigation aimed to evaluate of some faba bean genotypes for some morphological and agronomic traits in order to determine the promissing genotypes which can be used in breeding program for improving faba bean. The study carried out at El-Gemmeiza Agricultural Research Station in two successive seasons; 2016/2017and 2017/2018. 50 different landraces collected by the National Gene Bank (from different region of Egypt) and one commercial cultivar (Giza716) used as a check were used. Data analysis showed that there were highly significant differences in all studied characters among genotypes and replications except days to 50% flowering, plant height and number of branches per plant for replicates only. This indicates that there is wide diversity among genotypes which will enrich plant breeding programs to get high new commercial varieties and adapted to climate change of faba bean. The data for genetic parameters showed that the extent of phenotypic and genotypic variances diversed from one character to another according to result data from broad sense heritability (h2) estimates clears that there was graduation  for all of studied characters with values from ranged 73.92 % to 95.03 %.which indicate that the effects of environment were lower than genetic effects inheritance of studied traits. In addition, high estimates of heritability could be successful for improving faba bean. Results of mean performance note that although commercial cultivar Giza 716 was outweigh for days to flowering and maturity traits, L47 recorded the highest mean values for 100-seed weight and seed yield/plant. In addition, L39 recorded the highest mean values for number of pods and num ber of seeds/plant. So lines 39 and 47 may be used in breeding programs for improving the performance of seed yield characters. The results of correlation coefficient showed that there were thirteen significant and highly significant positive phenotypic correlation in addition, four significant and highly significant negative phenotypic correlation. The direct contribution of 100- seed weight per plant had highest mean values followed by number of seeds per plant. Number of seeds per plant had the highest indirect effect via number of pods per plant,  and number of branches per plant via number of seeds per plant. In addition, 100 seeds weight via number of branches per plant, 100-seed weight also had an appreciable indirect effect via leaflet width and number of branches per plant. Cluster analysis for investigated traits showed diversity among investigated fifty-one faba bean genotypes. All genotypes divided into two groups at a distance of 5.924. The first group contain line 47. The second group contains other genotypes.  100-seed weight and seed yield per plant are valuable in splitting the studied genotypes into two groups high value included the first group, however low vale included the second group.

In this study, Two field experiments were conducted in Ourabi operation Farm, Cairo-Ismaellia desert road at Kalubia Governorate. The experiment was carried out during the two seasons of  2013/2014 and 2015/2016, to investigate the effect of  plant growth regulator (Mepiquat chloride) with three different potassium fertilizers sources [chemical potassium (KC), foliar potassium (KF) and rock potassium (KR)] on growth development and total yield in potato plants (Solanum tuberosum L. cv. Spunta) . Chemical potassium (control) with / without Mepiquat chloride achieved better plant height, no. of stem and leaf area/plant. Rock potassium with mepiquat chloride  obtained the highest productivity of fresh and dry weights, total tuber yield and yield components  (weight of tubers, size of tubers, diameter of tubers and number of tubers) following by foliar potassium with mepiquat chloride  compared with control treatment.