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EVALUATION OF PHYTOCHEMICAL COMPOUNDS IN Moringa oliefera Lam. EXTRACTS ON CYTOTOXICITY OF MCF-7 CELL LINE Full text
2019
Marwa Abd El Alem | S. Ali | M. Sarwat | S. Hussein
Moringa olifera Lam. which called "Miracle tree" was an enormous usage of medical, nutritional and economic benefits so its leaves and roots extracts were vitro examined for phytochemicals contents, antioxidant, and cytotoxicity against MCF-7 cell line. Leaves and roots both extracts were estimate using 2,2-di phenyl-1-picryl hydrazyl (DPPH) radical scavenging assay and high performance liquid chromatography (HPLC) to determine phenolic and flavonoids compounds. The leaves aqueous and ethyl acetate extracts contained a significantly (p<0.05) more phenolic compounds than root extracts, while roots ethyl acetate extracts were significantly more active in DPPH assay comparing with aqueous extracts . However, the leaves aqueous extract and roots ethyl extracts were contained high values flavonoids compound but in case of phenolic compounds the aqueous roots ex tract recorded the greatest highest number followed by the leaves ethyl acetate extract. The ethyl acetate extractions for leaves and roots have a high inhibition percentage against breast adenocarcinoma MCF-7 cell line comparing with aqueous extracts.
Show more [+] Less [-]EFFECT OF SPIRULIN (ARTHROSPIRA PLATENSIS) A AND NANNOCHLOROPSIS (NANNOCHLOROPSIS GADITANA) SUPPLEMENTATION ON GROWTH PERFORMANCE, FEED UTILIZATION AND CARCASS COMPOSITION OF NILE TILAPIA (Oreochromis niloticus) Full text
2019
zeinab ali | T. Ali | M. Osman
The study was carried out at Faculty of Agriculture, Ain Shams University to assess the effect of two of algae species (Arthrospira platensis and Nannochloropsis gaditana) on growth performance of monosex tilapia fish (Oreochromis niloticus), fish were fed on a basal diet supplemented with 0,3,5,7% of each algae. To perform seven experimental treatments were assigned in three replicates each. The experiment was designated as follows: (T1) control (without algae), (T2) basal diet supplemented with 3% spirulina (spiru 3), (T3) basal diet supplemented with 5% spirulina (spiru 5), (T4) basal diet supplemented with 7% spirulina (spiru 7), (T5) basal diet supplemented with 3% Nannochloropsis (nanno 3), (T6) basal diet supplemented with 5% Nannochloropsis (nanno 5) and (T7) basal diet supplemented with 7% Nannochloropsis (nanno 7). Experimental tanks were a part of closed recirculating system, where almost constant environmental conditions were kept throughout the experimental period. Twenty-one quadrate fiber glass tanks with 108-liter water capacity were stocked with 15 fish per tank. The individual initial body weight (2.7 g) was recorded at the beginning of the experiment. All fish in each tank was weighed every two weeks during the whole experimental period. The fish were fed the experimental diet for 95 days Results indicated that nanno 5 treatment had higher significant trend (P<0.05) final body weight (FBW), average weight gain (AWG) and average daily gain (ADG) compared with the other experimental treatments. The best specific growth rate (SGR), feed conversion ratio (FCR) and feed efficiency ratio (FER) were found in nanno 7. Also, it was observed that spiru 7 have a high significant difference (P<0.05) in protein efficiency ratio (PER) and protein productive value (PPV). On the other hand, the results showed that the lowest AWG, ADG and (FER) appeared in spiru 7%. No mortality in nanno7 and spiru 7. Finally, using of nano 5% or spiru 7% enhance growth performance and feeding in tilapia fish.
Show more [+] Less [-]ASSESSMENT OF SOME AGRO-MORPHOLOGICAL TRAITS IN GENOTYPES OF EGYPTIAN FABA BEAN (VICIA FABA L.). Full text
2019
M. El-Shal | Azza El-Sayed
This investigation aimed to evaluate of some faba bean genotypes for some morphological and agronomic traits in order to determine the promissing genotypes which can be used in breeding program for improving faba bean. The study carried out at El-Gemmeiza Agricultural Research Station in two successive seasons; 2016/2017and 2017/2018. 50 different landraces collected by the National Gene Bank (from different region of Egypt) and one commercial cultivar (Giza716) used as a check were used. Data analysis showed that there were highly significant differences in all studied characters among genotypes and replications except days to 50% flowering, plant height and number of branches per plant for replicates only. This indicates that there is wide diversity among genotypes which will enrich plant breeding programs to get high new commercial varieties and adapted to climate change of faba bean. The data for genetic parameters showed that the extent of phenotypic and genotypic variances diversed from one character to another according to result data from broad sense heritability (h2) estimates clears that there was graduation for all of studied characters with values from ranged 73.92 % to 95.03 %.which indicate that the effects of environment were lower than genetic effects inheritance of studied traits. In addition, high estimates of heritability could be successful for improving faba bean. Results of mean performance note that although commercial cultivar Giza 716 was outweigh for days to flowering and maturity traits, L47 recorded the highest mean values for 100-seed weight and seed yield/plant. In addition, L39 recorded the highest mean values for number of pods and num ber of seeds/plant. So lines 39 and 47 may be used in breeding programs for improving the performance of seed yield characters. The results of correlation coefficient showed that there were thirteen significant and highly significant positive phenotypic correlation in addition, four significant and highly significant negative phenotypic correlation. The direct contribution of 100- seed weight per plant had highest mean values followed by number of seeds per plant. Number of seeds per plant had the highest indirect effect via number of pods per plant, and number of branches per plant via number of seeds per plant. In addition, 100 seeds weight via number of branches per plant, 100-seed weight also had an appreciable indirect effect via leaflet width and number of branches per plant. Cluster analysis for investigated traits showed diversity among investigated fifty-one faba bean genotypes. All genotypes divided into two groups at a distance of 5.924. The first group contain line 47. The second group contains other genotypes. 100-seed weight and seed yield per plant are valuable in splitting the studied genotypes into two groups high value included the first group, however low vale included the second group.
Show more [+] Less [-]EFFECT OF MEPIQUAT CHLORIDE AND POTASSIUM SOURCES ON GROWTH AND YIELD OF PATATO PLANTS (SOLANUM TUBEROSUM L.) Full text
2019
Ali Abo Al-Nagaa
In this study, Two field experiments were conducted in Ourabi operation Farm, Cairo-Ismaellia desert road at Kalubia Governorate. The experiment was carried out during the two seasons of 2013/2014 and 2015/2016, to investigate the effect of plant growth regulator (Mepiquat chloride) with three different potassium fertilizers sources [chemical potassium (KC), foliar potassium (KF) and rock potassium (KR)] on growth development and total yield in potato plants (Solanum tuberosum L. cv. Spunta) . Chemical potassium (control) with / without Mepiquat chloride achieved better plant height, no. of stem and leaf area/plant. Rock potassium with mepiquat chloride obtained the highest productivity of fresh and dry weights, total tuber yield and yield components (weight of tubers, size of tubers, diameter of tubers and number of tubers) following by foliar potassium with mepiquat chloride compared with control treatment.
Show more [+] Less [-]AN ASSESSMENT STUDY OF GROWTH PERFORMANCE AND GONADS DEVELOPMENT OF MONO SEX NILE TILAPIA IN DIFFERENT AGE STAGES DURING THE PRODUCTION PERIOD Full text
2019
Sara El-Nahal | M. Amer | M. Osman | T. Ali
The objective of this study was to follow up the growth performance and gonads development of sex reversed male Nile tilapia. Fish fed commercial diet contained 30% crude protein at a rate of 3% of the biomass and 10 fish were randomly taken biweekly during the experimental period (16 weeks). Growth performance, feed utilization and survival rate were calculated. Histological examination of the gonads was done every two weeks to follow the growth and development of the gonads. Results showed positive interactions between growth parameters and reproductive development during different periods of fish productive cycle. Histological examination illustrated that during early ages, testicular sections of mono sex male’s tilapia had abnormal architecture with deterioration of germinative tissue. Despite abnormal testis texture, fish started spermatogenesis, a step toward puberty when their body weight reached 29.16g. As fish reached 4-5 months, male start to recover their testes normal structures and at age of 5.5-6 months, testicular sections appeared normally as pointed out by firmed testicular lobules, existence of all germ cell types and the intensively stored spermatozoa in testicular lumen and testicular ducts, confirming the full maturity of males. Based on the obtained results, it can clearly conclude that there is a relationship between age, body weight and development of sexual glands. It has also been shown that the hormonal treatment of tilapia seeds in the early stages for production of mono sex (all males) leads to a marked deterioration in the testes structure, continues near the fish enters the sexual maturity. At a later age, males can overcome this deterioration, restore the histological structure of the testes and achieve full sexual maturity at the age around from 5.5 to 6 months.
Show more [+] Less [-]In vitro MATURATION OF DROMEDARY SHE-CAMEL OOCYTES EXPOSED TO LASER IRRADIATION Full text
2019
Maiada Allam | E. Abdalla | F. Khalil | A. Zeidan | Z. Abdel-Salam | M. Badr
The objective of this study was to study the effect of laser irradiation on maturation rate of dromedary she-camel oocytes. Although in vitro fertilization (IVF) technique in she-camel has been established, but maturation rate of camel oocytes is still low comparing with other animal species. Several studies performed to improve in-vitro maturation rate using different types of media with different incubation times. In order to establish high sensitive and low cost maturation improvement technique, laser irradiation has been suggested in the present work. Cumulus oocytes complexes (COCʼs) were collected from ovaries by aspiration method and grade (A) oocytes were chosen and divided into five different groups, 62 oocytes served as control group, an un-irradiated (group 1), 64 oocytes exposed to 2 minutes of laser irradiation (group 2), 57 oocytes exposed to 3 minutes of laser irradiation (group 3), 49 oocytes exposed to 4 minutes of laser irradiation (group 4) and 52 oocytes exposed to 5 minutes of laser irradiation (group 5) with a total output power of 3 mW for different exposure durations; 2, 3, 4 and 5 minutes. Afterwards, oocytes were matured in TCM-199 medium at 38.5oC and 5% CO2 in humidified air for 42 h. Maturation rate was calculated based on expulsion of the first polar body and statistically analyzed by one way ANOVA test. The obtained results showed that, the oocytes reached germinal vesicles (GV) which exposed to laser beam for 5 minutes at 488 nm wavelength represent significantly (P<0.05) the highest value (42.31%) compared to control (not irradiated, 16.13%). However, other groups of GV showed insignificant differences with the control group. The metaphase II (M II) in the control oocytes represents significantly (P<0.05) the highest value (75.81%) compared to 3-5 minutes exposed groups. The degenerated oocytes exposed to laser beam for 5 minutes at 488 nm wavelength represent significantly (P<0.05) the highest value (40.38%) compared to control (not irradiated, 8.06%). In conclusion‚ these results indicated that the exposure of laser irradiation for 2 minutes may improve in-vitro nuclear maturation of immature oocytes in dromedary she-camels as compared to other durations (3-5 minutes) at 488 nm wavelength (blue laser).
Show more [+] Less [-]EFFECT OF SOME AGRICULTURAL PRACTICES ON IMPROVING SALTS AFFECTED SANDY SOIL PRODUCTIVITY. Full text
2019
Diia Boulos | Hoda Elia
A field experiment was conducted on sandy soil at Ismailia Governorate. The aim of the present study is to improve the productivity of salts affected sandy soil by treat it with different rates of bentonite, chicken manure and their combination. Soil application rates were bentonite (1, 2.5 and 5 %), chicken manure (5, 10 and 15 ton/fed) and their combination (2.5% bentonite with each of 5, 10 and 15 ton /fed chicken manure) were mixed in the upper soil layer (0-15 cm soil depth) and their effects on some soil physical properties and crop productivity of fennel plant (Foeniculum Vulgare Mill.) were investigated during the winter season of 2018. The results revealed that, all studied treatments were significantly decreased of soil bulk density compare to control. The applications of bentonite 1, 2.5 and 5% lead to, reduction of the value of soil bulk density by 4.17, 5.95 and 10.71%. In addition, the applications of chicken manure 5, 10 and 15 ton/fed, lead to reduction of the value of soil bulk density by 8.93, 11.31 and 14.88 %. Also, application of 2.5% bentonite combined with each of 5, 10 and 15 ton/fed chicken manure, lead to reduction of the value of soil bulk density by 11.31, 15.48 and 17.86% as compared with control, respectively. Data indicated also that there are an increase in the percentages of retained moisture in the soil at all treatments under study. Bentonite and chicken manure individually or combined being higher with increasing the applied rate of bentonite and/or chicken manure. Also the application rates of bentonite 1, 2.5 and 5% lead to, increasing the values of water-holding pores (WHP) with 6.87, 8.05 and 10.55%. In addition, WHP values increased to 9.21, 10.43 and 11.27% with the application of chicken manure 5, 10 and 15 ton/fed. Also, data refer that WHP increased to 12.03, 12.81 and 14.11% by application rate of 2.5% bentonite combined with each of 5, 10 and 15 ton/fed chicken manure, as compared with control, respectively. The values of water infiltration rates (cm/h), were affected by different soil applications and can be arranged in the following ascending order: 2.5% bentonite + 15 ton/fed chicken manure < 5% bentonite < 2.5% bentonite + 10 ton/fed chicken manure < 2.5% bentonite < 15 ton/fed chicken manure < 2.5% bentonite + 5 ton/fed chicken manure < 10 ton/fed chicken manure < 5 ton/fed chicken manure < 1% bentonite < control. The water use efficiency values, affected by different soil application can be arranged in the following descending order: 2.5% bentonite + 15 ton/fed chicken manure > 2.5% bentonite + 10 ton/fed chicken manure > 2.5% bentonite + 5 ton/fed chicken manure > 15 ton/fed chicken manure > 10 ton/fed chicken manure > 5 ton/fed chicken manure > 5% bentonite > 2.5% bentonite > 1% bentonite > control.
Show more [+] Less [-]Effect of chitosan and salicylic acid as exogenous elicitors on growth and biochemical constituents of broccoli seed sprouts Full text
2019
Rasha Bondok | Ahmed Abdel-Hafez | Hany Metwally | Zeinab Abdeghany
The effect of chitosan and salicylic acid treatments to investigate the seedling growth parameters of Broccoli seed sprouts (Tiburon f1) cultivar was the aim of this study. Salicylic Acid and Chitosan are the )elicitors( that stimulate the sprout, which are divided into Biotic (biological origin), abiotic (chemical or physical origin) elicitors and phytohormones have been applied alone or in combinations, in hydroponic solutions or sprays, and in different selected time points of the sprout growth or during post-harvest. Cumulative time had been completed (harvest time) using tap water, chitosan at 25, 50,100 ppm and salicylic acid at 65,130,260 ppm for seed soaking and sprouting , then precooled and air-dried sprouts were randomly chosen from glass jar (replicate) to collect data of sprout length, hypocotyl length, radical length, dry weight and fresh weight. Also, Samples of harvested 3, 5 and 7 days old etiolated broccoli dried using air draft oven at 65.5C° then grounded into powder for chemical analysis while fresh sprouts and seeds were analyzed for total phenols. However treatment with chitosan at 100 ppm cleared that the moisture, protein, ash, fiber, calcium , potassium, and total phenols of sprouts were increased by 9.63%, 33.73%, 11.80, 11.67%, 336.50 ppm, 196.43 ppm and 66.51mg/100g, respectively. Treated sprouts with salicylic acid at 65 ppm concentrate were also induced an increments on the same constituents reached 9.53%, 33.40%, 11.63, 10.46%, 333.50 ppm, 190.60 ppm and 62.90mg/100g, respectively over control (untreated). Therefore, treated sprouts with chitosan or salicylic acid could be considered as useful tool for improving the growth characters and bioactive metabolites of Broccoli seed sprouts For their Production of anti-cancer materials, As well secondary metabolic pathways respond to specific treatments with elicitors would be the basis for to enhance the production of secondary metabolites, in order to produce quality and healthy fresh foods
Show more [+] Less [-]Studying Rain Water Catchment Potentialities in the Northwest Coast of Egypt Using Remote Sensing and Geographic Information System Full text
2019
Asmaa Khater | Ayman Abouhadid | Usama Elbehairy | Mohamed Aboelghar | Abdelraouf Aly
Sustainable agricultural development of the desert areas of Egypt under the scarcity of irrigation water is a significant national challenge. Existing water harvesting techniques on the northwest coast of Egypt do not ensure the optimal use of rainfall for agricultural purposes. Basin-scale hydrology potentialities were studied to investigate how available annual rainfall could be used in agriculture irrigation to increase crop production. The present study includes data related to agricultural production in the form of geospatial layers including climate, soil, land covers unite and rain water catchment areas. Thematic classification of Sentinal-2 imagery was carried out to produce the land cover and crop maps following based on the (FAO) system of land cover classification. Contour lines and spot height points were used to create a digital elevation model (DEM). Then, DEM was used to delineate basins, sub-basins, and water outlet points using the Soil and Water Assessment Tool (Arc SWAT). Main soil mapping units of the study area identified from Land Master Plan maps it was(Very shallow gravelly and rocky soils and barren rock). Climatic data collected from the Central Laboratory for Climate. The results showed that the study area receives a significant amount of precipitation almost every three years, however, water harvesting methods are inappropriate to store water to be used in agricultural during drought seasons. The amount of precipitation(81.9 mm), surface water runoff(4.46 mm), potential evapotranspiration(70.5 mm), and actual evapotranspiration(7.10 mm) for the years (2004 to 2017) shown as results of (Arc SWAT). The land cover map showed that tree crops (olive and fig) cover 195.8 km2 when herbaceous crops (barley and wheat) cover 154 km2. The maximum elevation was 250 meters above sea level while the lowest one was -3 meters below sea level. The study area receives a massive variable amount of precipitation; however, water harvesting methods are inappropriate to store water for purposes.
Show more [+] Less [-]POSSIBILITY OF USING AGRICULTURAL WASTE IN EGYPT "A CASE STUDY IN GHARBIA GOVERNORATE" Full text
2019
Neveen Aly | M. El-Eraky | Salwa Abd Elmoneim | M. El-sawy
It is estimated that the rice cultivated area in Egypt is estimated at about 1.4 million feddans producing about 3 million tons of rice straw. The same estimates indicate that the cultivated area of summer maize is estimated at 2.1 million feddans, which produces about 5.5 million tons of woodland (in 2013), Most of which are burned and not the maximum benefit. The problem of research is to increase the amount of agricultural waste, especially for rice and maize crops, and not to achieve the economic efficiency of these quantities of agricultural, And the study of the development of the area cultivated for each of the maize, cotton, sesame, sorghum, lupine, black beans, sugar beet, rice and the total of these crops in Egypt during the period (2000-2016) the annual average of about 324.44, 460.06, 72.66, 354.76, 4.07, 1471.2, 298.88, 1452.41, 3114.4 and the total area of the maize crop during the study period. The statistical significance of these equations was proved, while the sesame yield was not substantiated. The development of the production capacity of the most important plant waste was studied in Egypt during the period 2000-2016. The average annual production capacity of the maize, cotton, sesame, sorghum, lupine, peanut, sugar beet, rice straw, These crops are 5586.12, 871.06, 94.47, 4.53, 235.65, 1711.65, 3217.06, 3217.06 and 2634.6 tons respectively during the study period. The general trend equations showed a decrease in the production capacity of cotton, sorghum and thermos. While the production capacity of each of the sesame, peanut bean and sugar beet was increasing. The statistical significance of these equations was not proved, nor was the statistical significance of the production of maize, rice straw and total production capacity of these crops. The cost of feeding was about 13505.1 pounds, representing about 78.3% of the total average cost of the head, representing about 96.1% of the total average variable costs of the head of about 14049.2 pounds, and amounted to about 17574.8 pounds without the use of silage, which Indicates that the use of silage in nutrition leads to a reduction in feeding costs by about 4069.7 pounds per year, This is due to the lower daily costs of feeding the head of cows when using silage in feeding. The cost of producing a ton of milk for dairy cows that used silage was about 3849 pounds, while the cost of producing milk for the head of dairy cows that did not use silage for about 13715.1 pounds, The production of milk is about 2805.4 kg, and thus the cost of producing a ton of milk for dairy mixtures that did not use silage about 4888 pounds, due to lower feeding costs for animals that used silage than not used silage in feeding.
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