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EFFECT OF CERTAIN PSEUDOMONAS FLUORESCENS ISOLATES ON THE INFECTION OF ROOT-KNOT NEMATODE, MELOIDOGYNE INCOGNITA IN TOMATO AND EGGPLANT AND THE PLANT GROWTH
2020
Nora Saleh | A Mahgoob | Entsar Taha | Wafaa El-Nagdi | M Youssef | Mona Zayed
Under screen house conditions, two experiments were carried out to evaluate certain bacterium, Pseudomonas fluorescens isolates regarding reproductive potential of root-knot nematode, Meloidogyne incognita, infecting tomato or eggplant. Results on tomato revealed that, on the basis of average total percentages nematode reduction, the over topped results were gained with P. fluorescens (Pf2) which recorded the highest significant (P≤0.05) average nematode reduction (61.3%) and higher percentage reduction of females (77%) per plant. The second rank was obtained by Pf3 which reduced all nematode numbers as an average of 56.9%.On the basis of average total percentages plant growth and weight of fruit increases, four bacterial treatments can be ranked in a descending order as follows: Pf9 > Pf4>Pf1and Pf7, as they achieved the highest average total percentages increases of 96.0, 47.3, 38.2and 29.8%, respectively compared to other treatments and untreated check. Regarding to eggplant, the over topped results observed was achieved by P. fluorescens (Pf10) which recorded the highest average total nematode reduction (66.2%) with higher reduction of (J2s) in roots (89.9%) per plant and in soil (78.8%) per pot. The second rank was obtained by Pf9 and Pf2 where they reduced all nematode numbers as averages of 55.9% and 54.9%, respectively. Also Pseudomonas isolates enhanced the plant growth of eggplant, averages were found in a descending order as follows: Pf1 (20.0%), Pf9 (18.7%) and Pf10 (18.3%) . It is worthy to note that the most distinct growth criteria was fresh weight of roots as it achieved higher percentage increase (58.1%) by using Pf9 followed by Pf1 as it caused 40.6% increase compared to untreated check. The highest average percentage of fresh weight of shoot were recorded for Pf10 (26.4%) and Pf8 (22.1%). Whereas for dry weight Pf3 (29.8%) and Pf2 (19.1%). In conclusion, the tested biocontrol agent was efficient in controlling the root-knot nematode on tested plants.
Show more [+] Less [-]IMPACT OF PINK PIGMENTED FACULTATIVE METHYLOTROPHIC BACTERIA AS A BIO-STIMULANT ON GROWTH, HERB YIELD AND VOLATILE OIL ATTRIBUTES OF ROSEMARY (ROSMARINUS OFFICINALIS L.) PLANT
2020
Doaa Soliman | A Hosni | A Abdel Hamid | Mona Mohamed
The effect of Pink Pigmented Facultative Methylotrophic (PPFM) bacteria as a bio-stimulant on vegetative growth, macro-nutrient elements ( N,P and K ) contents , chemical constituents and volatile oil percentage and components in rosemary plant (Rosmarinus officinalis L.) were studied during 2018 and 2019 seasons. Plant height (cm), number of lateral branches/plant, herb fresh & dry weights and root fresh & dry weights of rosemary plant were all greatly affected with all applied treatments of PPFM when compared with the control. The hugest effect was recorded by 1% PPFM bacteria spraying in both studied seasons. However, no significant effect was recorded due to PPFM applied treatments on P%. Highest values of total carbohydrates were recorded by both 1 and 3%. PPFM bacteria spraying in both studied seasons. Whereas, the highest values of C/N ratios were obtained with 3% PPFM in first season and 1% PPFM in the second one. A substantial effect from PPFM bacteria spraying was observed through its effect on reducing the level of NO3 and NO2 in rosemary herb when compared with the control. In any case, 3% of PPFM bacteria spraying was more effective than other treatments in recording the least values of NO3 and NO2 in first season compared to the control. Volatile oil % was enormously increased with 3% PPFM bacteria spraying in both studied seasons than other treatments or control. The main components of volatile oil of rosemary plant were Camphor, Eucalyptol, Verbenone, Borneol, Pinene, Pinanone, Bornyl acetate and Camphene. The treatments of PPFM bacteria spraying immensely stimulated the component % in volatile oil of rosemary herb than in the control. It could be concluded that the application of PPFM bacteria as foliar spraying in rosemary plant production had many advantages especially reducing NO3 and NO2 levels and increasing volatile oil % and volatile oil fractions not to mention it increased the percent of active ingredients.
Show more [+] Less [-]Production of Function yogurts drink fortified with different types of herbal extracts and study its biological attributes in hepatitis rats
2020
M Al-Soud | O Elbatawy | A Abdel Fattah | Safaa Gohari | W El-Dsouky
The present study aimed to produce function yoghurt drink fortified with different types of herbal extracts (Ginger, Amla, Curcuma) and assess its therapeutic effect in hepatitis rats. The rats were randomly divided into two main groups. The first group were control (-) (6 rats) was fed on a standard diet, while the second group (30 rats) were fed on standard diet and injected it by CCl4 "for two weeks" to induce chronic damage in the liver (hepatitis) then divided into five groups (6 rats each group), then treated by plain drinking yoghurt and different herbal drinking yoghurt fortified with different types of herbs extract. Different Biological attributes were determined. The antioxidant activity, total phenolic content and chemical properties were evaluated in herbal extracts and yoghurt product and their hepatoprotective effect of each alone evaluated in albino rats against carbon tetrachloride (CCL4) induced liver injury. The hepatoprotective activity was assessed using various biochemical parameters and histopathological studies. The results indicated that functional flavoured drinking yoghurt containing herbal extract exhibited no significant differences were observed in total solids, fat, protein, ash, and lactose content them control samples. Drinking yoghurt fortified with Curcuma exhibited highest content of antioxidant activity and total phenolic content among all treatments. Also, rats fed on diet supplemented with drinking yoghurt fortified with Curcuma for 28 days showed higher potential hepatoprotective effects compared to liver injury control group (IC). The rats succeeded to restore the biochemical parameters and improved the histological alteration of the liver. This improvement was pronounced partly in the group received drinking yoghurt fortified with Ginger and Amla herbal extracts while, the group received drinking yoghurt fortified with Curcuma herbal extract were improved totally. It could be concluded that drinking yoghurt fortified with different herbal extract especially Curcuma can be used as ingredients in functional foods for people suffering from liver diseases.
Show more [+] Less [-]AN ECONOMETRIC ANALYSIS OF FISH FOOD SECURITY IN EGYPT
2020
Seham Hashem | Ashraf Younis Shebl
The problem of fish food security is represented in insufficient strategic stocks of fish, especially in light of the increasing demand for fish, and lower production than consumption. The research mainly targeted the study of the situation of fish food security in Egypt, in addition to a set of sub-goals, which are the most important that the estimation of both the strategic stock of fish and fish food security coefficient in Egypt, studying of the most important factors affecting fish food security, studying of future expectations for fish food security, This is in addition to studying the problems that Obstruct the continued increase in fish production. The research relied on achieving goals on the use of qualitative statistical methods and economic equations for estimating both strategic stocks and security coefficient for fish. As Quantitative statistical methods were used, which are represented in using the simple and multiple regression method to estimate the functions of consumption, production, import and export of Egyptian fish, as well as food security coefficient in order to determine the most important variables that affect it by building an econometric model that explains the determinants of food security of fish. The double logarithmic model was the best model used in terms of economic logic and statistical significance, and Autoregressive and Integrated Moving Average (ARIMA) model was used to forecast production, consumption, imports and food security coefficients for fish until 2025. In addition to using the statistical method, One-way Anova- Analysis of Variance, to test the differences between a number of independent groups that represent problems that impede the continued increase in fish production, as well as the use of the least significant difference method (L.S.D) to arrange the elements of each problem. By estimating the growth rate of fish production and consumption, the fish food gap and the average per capita share, it was found that the growth rate of each of them took an increasing general trend during the study period, where the annual growth rate is estimated at 5.4%, 4.9%, 2.8%, and 2.7% for each of them, respectively. It was also found that the quantity of the surplus in fish attained about 0.9 thousand tons on average during the study period, and this surplus is very small, not enough consumption per day. It is also clear that the fish food security coefficient reached about 0.00073 on average during the period (20002018) and this indicates a decrease in the fish food security rate. The forecast values of the fish food security coefficient have shown that the fish food security coefficient tends to decrease from about 0.00076 in 2019 to about 0.00073 in 2025. And by studying the most important variables that affect fish food security in Egypt through the three basic functions, which are the fish demand function, the fish supply function, and the fish food security coefficient function, of the Simultaneous model, according to the economic logic and statistical significance, Increase in both national income (x13), imports of fish (Y3t), local production of fish (Y1t) by 1%, the food security coefficient of fish increases by 0.04%, 0.102%, 0.49% for each of them respectively, and when increasing Consumption (Y2t) by 1%, the fish food security coefficient decreases by 0.67%. This has proven the significance of the variables at the level of significance 0.01, as it turns out that by increasing exports (Y4t) by 1%, the food security coefficient decreases by 0.01% and this decrease is statistically significant at the level of significance 0.05. A stratified random sample was chosen according to the cognitive and functional level, with the problems and obstacles of continuation of increasing fish production in Egypt. The sample was of those interested in the field of fish wealth. The problems were identified and divided into five categories which are: environmental problems, financing and production problems, marketing problems, problems related to scientific research, administrative problems. The study resulted in several recommendations, the most important of which are: Increasing the expansion of culture operations in floating marine cages. Increasing investment orientation for fish production from its various sources and providing financial support to small fish producers. Close control of the various pollution image processes of natural resources, overfishing, offensive fishing, and fishing fry and limiting their spread to preserve biological stocks.
Show more [+] Less [-]Genetic Improvement of Some Productive Traits in Zaraibi Goats
2020
Rasha Ahmed | Mona Osman | Manal Elsayed | Hussein Mansour
The objective of this study was to evaluate the genetic improvement in Zaraibi goats herd raised on El-serw research station located in the north eastern part of Nile Delta, Egypt. The station belongs to Animal Production Research Institute (APRI). Some body weights at different ages were included in genetic and environmental trends. These weights were 4-month weight (4M), 6-month weight (6M), and 12-month weight (12M). Data and pedigree information were collected from 1988 until 2018. The least square means of body weights at 4M, 6M and 12M were 12.14±0.02, 15.49±0.02 and 24.08±0.04 kg, respectively. Year of birth, season, gender of kid, parity of dam and type of birth significantly (P<0.01) affect growth traits. Genetic parameters were estimated using a multi-trait animal model program (MTDFREML). The same previous fixed effects were included in this analysis regarding the animal as random effects. The heritability of the studied body weights tend to increase as age increases. That is inversely matched with the environmental proportion of total variance which was found to be 0.72, 0.66 and 0.62 for M4, M6 and M12, respectively. The heritability value was estimated as 0.28 for 4M. Genetic trends were obtained as the regression of the predicted breeding values on years of birth and Environmental trends were obtained as the regression of year constants on years of birth. The annual genetic change trends were positive and significant for 4M, 6M and 12M (0.091, 0.121 and 0.158 kg/year, respectively) and the annual phenotypic change trends were (0.020, 0.045, 0.117 kg/year, respectively). Genetic trends for M12 trait were higher than those for other traits in this study. Genetic and environment correlations between 4 month body weight and other growth traits were positive. Negative environmental trends indicates poor management system for the flock during studied period. Weight at 12 months of age has the highest heritability in studied body weights and suggested to be selection criteria to improve yearling weight for Zaraibi goat.
Show more [+] Less [-]OPTIMIZATION OF AMINO ACID PRODUCTION FROM CHICKEN FEATHER BY BACILLUS AMYLOLIQUEFACIENS USING RESPONSE SURFACE METHODOLOGY AS A NEW TOOL FOR POWDERY MILDEW DISEASE BIOCONTROL
2020
Samah Abu-Hussien | Maha Mohamed
The present work aimed to optimize amino acids production by Bacillus amyloliquefaciens 35s from chicken feather using response surface methodology (RSM) and applying the produced amino acids in biological control of powdery mildew disease. Variables affecting amino acids production from B. amyloliquefaciens 35s were screened using Plackett Burman design. Factors showed to be significant to produce amino acids were optimized using central composite design (CCD) of RSM at three coded levels (-1, 0, +1). All obtained data were analyzed by ANOVA with post hoc multiple comparison analysis performed using Tukey’s HSD. TGY (tryptone, glucose, yeast extract) was the basal medium. Impacts of medium components were studied using Plackett-Burman design. “Design Expert 12” StateEase was used to analyze the experimental Plackett- Burman design. Feather, yeast extract, CaCO3, KH2PO4 and agitation rate (using shake flask) were optimized statistically by the CCD design of the RSM. Validation of statistical model of the medium components was done by carrying out the experiment at optimum conditions of the process parameters as determined by the model. Amino acids types were identified by amino acid analyzer. Among the significant medium components, feather, yeast extract, CaCO3, KH2PO4 and agitation rate showed to have significant effect on amino acids production. Predicted maximum amino acids production was observed near the mid-point concentrations of the mentioned above factors and the experimental value (6.55 g/l) was very close to the predicted value of (6.60 g/l) predicted by the model. Amino acid analyzer analysis for the produced amino acids shows the release of asparagine, threonine, serine, glutamine, proline, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, phenyl alanine, histidine, lysine and arginine, respectively. Effect of chicken feather hydrolysate on cucumber powdery mildew disease was tested by applying foliar application with different concentrations (0, 5, 10, 15, and 20% v/v), then degree of infection and sporulation on cucumber plants were assessed. The lowest concentration of chicken feather hydrolysate showed the lowest effect on disease index. Conversely, cucumber resistance increased by the increasing of feather’s hydrolysate concentrations associated with a clear reduction in the disease index and sporulation. Aside from, extra foliar application showed a decrease in the disease index for all concentrations compared with untreated plants. Foliar application with 20% concentration of chicken feather hydrolysate had the highest stimulation of the studied oxidative enzymes i.e., peroxidase (POX), phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and superoxide dismutase (SOD).
Show more [+] Less [-]POPULATION STRUCTURE AND GENOME-WIDE ASSOCIATION ANALYSIS FOR SALINITY TOLERANCE IN BREAD WHEAT USING SNP, SSR AND SCOT MARKER ASSAYS
2020
Alsamman Alsamman | S.D. Ibrahim | Mohamed Rashed | Ayman Atta | M.S. Ahmed | Alaa Hamwieh
Wheat is an essential staple food in the developing world, where demand is projected to grow exponentially in the future; simultaneously, climate changes are projected to reduce supply in the near future. One of the main consequences of climate change is salinity, which negatively impacts the world's cultivated area and therefore affects the global wheat production. Our objectives are to study the population structure of several Egyptian and international wheat accessions and to identify the genetic factors controlling the salinity stress response of bread wheat. In addition, we have attempt to identify genes that control some important agronomic parameters of wheat under salinity stress were identified. The wheat germplasm panel consisted of 70 accessions obtained from Egypt, Syria and Iran. The assessment of salinity tolerance was conducted over the years of 2018 and 2019 in the field and in the greenhouse. The genome association analysis (GWAS) and population structure analysis was conducted using six SCoT, five SSR and 93 SNP markers. Analysis of the population structure using allele frequency and phylogenetic analysis indicated that the studied wheat accessions were belong to four population groups. Where, for the most portion, Egyptian, Syrian and Iranian accessions are clustered depending on their country of origin. The GWAS analysis revealed 13 SNP markers that were significantly associated with morpho-agronomic wheat traits during salinity stress. These markers were closely related to genes that are known to have a direct link to wheat response to salinity stress such as CYP709B2, MDIS2, STAYGREEN, PIP5K9, and MSSP2 genes. This study revealed the genetic structure of adapted and imported wheat accessions, which could be used to select potential wheat accessions for local breeding programs. In addition, the SNP genotyping assay is a very potential technology that could be efficiently applied to detect genes that control bread wheat response to salinity stress.
Show more [+] Less [-]EFFICIENCY OF THE AGRICULTURAL ECONOMIC RESOURCES USED IN SUMMER TOMATO PRODUCTION IN ALEXANDRIA GOVERNORATE
2020
Ahmed Kassem | Noura Tantawy | Tamer El-Santresy
The research aimed to measuring the Efficiency of the Agricultural Economic Resources Used in Summer Tomato Production in Alexandria Governorate, the research is relied on using of some descriptive and quantitative economic analysis methods in data analysis, especially Data Envelopment Analysis (DEA) method, based on field data collected during the 2020 season from 30 summer tomato's farmers in Alexandria Governorate. The research reached a set of results, the most important of which can be reviewed as follows: (1) The overall efficiency criteria for a feddan of summer tomatoes in the research sample are: the net return is about 12.52 thousand pounds, the income over variable costs is about 17 thousand pounds, the benefitcosts ratio is about 1.41, and the profit of the spent pound is about 0.41 pounds. (2) The average Scale Efficiency was about 95.6%, which means that the percentage of exploitation of economic resources used in production in summer tomato farms in the research sample was about 95.6%, indicating that there is a possibility of expansion of 4.4% to reach the optimum volume of production on these farms. (3) The average Technical Efficiency according to the (BCC) model is about 98.4%, and this confirms the possibility of increasing the summer tomato production by 1.6% without increasing the amount of economic resources used. (4) Inefficient farms can achieve complete technical efficiency as reference farms by reducing the economic resources used, which are: the human labor by 7.22%, the automated work hours by 8.36%, the seedlings by 2%, the fertilizer Municipal by 4.62%, nitrogen fertilizer by 4.39%, phosphate fertilizer by 4.96%, potassium fertilizer by 4.93%, and pesticides by 6.81%, while keeping the same volume of production of summer tomatoes research sample. (5) The average Allocative Efficiency of summer tomato farms was about 79.7%, which means that re-employing the used economic resources will save about 20.3% of the production cost through adopting better technology and optimal employment of resources. (6) The average Cost-Efficiency of summer tomato farms in the research sample was about 78.4%, which means that production costs can be reduced by about 21.6% of the current production costs without affecting the production level of summer tomatoes. (7) Achieving the technical efficiency of summer tomato production at the level of Alexandria Governorate the following: (a) Increasing production by 13.07 tons, with a monetary value estimated at 23.59 milliom pounds, (b) providing an area of agricultural land with a horizontal expansion of 586.12 feddans, which is equivalent to the increase achieved In production as a result of raising technical efficiency, (c) rationalizing the use of irrigation water in Alexandria, with a total of about 2.04 million m3 , which is the amount of savings achieved in irrigation water that is necessary for the area of land that has been provided in Alexandria. In light of the findings of the research, it recommends the necessity; (1) Educating summer tomato farmers about the optimal the economic resources used in production to reach the optimum volume of production, (2) Reduce the prices of agricultural production inputs and provide them in a timely manner so that the farmer does not resort to the black market, (3) Issuing advisory bulletins to confront the changing climate and high temperatures in summer tomato cultivation.
Show more [+] Less [-]دراسة اقتصادیة لأهم العوامل المؤثرة علی کمیة وجودة الواردات المصریة من القمح
2020
Nariman Farid | وحيد علي محمد مجاهد | mohamed othman
یعد محصول القمح من أهم محاصیل الحبوب الغذائیة فی مصر و یعتبر سلعة إستیراتیجیة، وتعتبر مصر أکبر مستورد للقمح علی مستوی العالم، ودائما تتجه الدول النامیة لإستیراد المواد الغذائیة التی تعانی عجزاً فی إنتاجها لسد العجز الغذائی لها و یلاحظ أن کمیة الواردات المصریة من القمح تزداد سنویا حیث زاد المتوسط السنوی لکمیة الواردات المصریة من القمح خلال فترة الدراسة (2001- 2018) من 6.9 إلی 11 ملیون طن، وقد ظهرت مشکلة البحث أن غالبیة الدراسات تهتم بالتعرف علی العوامل المحددة للکمیات الواردة من القمح و فی المقابل لایوجد إهتمام ملحوظ بالعوامل المحددة لجودة تلک الواردات لذلک إستهدف البحث الوقوف علی أهم العوامل المحلیة التی تؤثر علی الکمیات الواردة من القمح، و کذلک أهم العوامل التی یعزی إلیها أسباب الرفض لبعض الکمیات غیر المطابقة لمواصفات الجودة والسلامة ومن هذا الهدف الرئیسی تندرج مجموعة من الأهداف الفرعیة تتمثل فی دراسة تطور الواردات المصریة من القمح، دراسة التوزیع الجغرافی للواردات المصریة من القمح، التعرف علی أهم العوامل الداخلیة المؤثرة علی کمیة الواردات المصریة من القمح، الأسباب المختلفة للرفض وفق التشریعات و المواصفات القیاسیة المصریة ذات العلاقة. واوضحت النتائج أن أهم العوامل الداخلیة المؤثرة علی کمیة الواردات المصریة من القمح تتمثل فی السعر الإستیرادی والإنتاج المحلی وعدد السکان، حیث تبین من التقدیر الإحصائی وجود علاقة طردیة تتفق مع المنطق الإقتصادی بین کمیة الواردات من القمح و بین عدد السکان و ثبتت معنویتها إحصایاً حیث أنه کلما زاد عدد السکان بمقدار ملیون نسمة تزداد کمیة الواردات بمقدار معنوی إحصائیاً بلغ حوالی 0.32 ملیون طن، کما إتضح أیضاً وجود علاقة عکسیة متفقة أیضا مع المنطق الإقتصادی بین کمیة الواردات من القمح و الإنتاج المحلی ثبتت معنویتها إحصائیاً حیث أنه کلما زاد الإنتاج المحلی بمقدار ملیون طن تنخفض کمیة الواردات بمقدار معنوی إحصائیاً بلغ نحو 1.03 ملیون طن سنویاً. کما تبین أیضاً عدم ثبوت معنویة تأثیر متوسط قیمة الوحدة الإستیرادیة حیث أن القمح سلعة إستیراتیجیة لایمکن الإستغناء عنها. کما تبین انه خلال الفترة (2007 – 2018) رصد عدد الرسائل المرفوضة من أهم الدول الموردة للقمح لمصر وأسباب رفضها لعدم مطابقتها للمواصفات و التشریعات المصریة حیث بلغ إجمالی عدد الرسائل المرفوضة فی تلک الفترة تقریباً 2937 رسالة بکمیة بلغت حوالی 2664.87 ألف طن وفیما یتعلق بأهم أسباب زیادة نسبة المرفوض من الواردات المصریة من القمح تبین أنها ترجع إلی إرتفاع نسبة الشوائب التی تتمثل فی إرتفاع نسبة حبوب القمح التالفة هذه النسبة تبلغ حوالی 55.5% من إجمالی أسباب الرفض، بینما جاءت مشکلة المواد الغریبة الغیر عضویة فی المرکز الثانی بنسبة حوالی 42.7 % من إجمالی أسباب الرفض.
Show more [+] Less [-]Efficiency of certain insecticides & bio-products against Bemisia tabaci (Genn.) and Tetranychus urticae koch on potato plants under field conditions AT QALUBIA GOVERNRAT, EGYPT.
2020
ٍSamar Esmail | Shoukry Elrefai | Fatina Baiomy
A field experiment was conducted during autumn plantation in season 2017 at Qaha, Qalubiya Governorate to evaluate the efficiency of ten different control agents; four of them are biorational components (garlic extract, rosemary oil + sunflower oil, sesame oil and chili oil), three of them are natural pesticide (Techno Oil, Top9 and Berna Star), one agent of them is bio-pesticide (bio-fly) and two chemical pesticide (Blanch 48% SC, Mospilan 20% SP) in reducing the population densities of Bemisia tabaci and Tetranychus urticae on Hatma potato cultivar. The results indicated that there were significant differences between the ten tested compounds in reducing the population density of B. tabaci nymphs; Mospilan 20% SP was the most potent compounds in reducing the population density of B. tabaci nymphs causing the highest reduction percentage (83.58 %), while Top9 gave a low effect with reduction percentage (49.32%) and Bio-fly gave moderate reduction percentage (63.92%). These compounds could be divided into two groups according to the reduction percentage: 1st group contains Mospilan 20% SP, Blanch 48% SC (chemical pesticides), Berna Star and Techno Oil (natural pesticides), respectively. 2nd group contains on Bio-fly (bio-pesticide), sesame oil, garlic extract, chili oil, rosemary oil + sunflower oil (biorational components), and Top9 (natural pesticide) on B. tabaci nymphs. Results also revealed that there were significant differences between the mean of general reduction percentage of the ten tested compounds on T. urticae population. Mospilan 20% SP recorded the highest reduction percentage (73.1 %), (rosemary oil + sunflower oil) treatment recorded the lowest reduction percentage 41.97% and Top9 gave moderate reduction percentage (60.03%). These compounds could be divided into two groups: 1st group contains on Mospilan 20% SP, Techno Oil, Blanch 48% SC, garlic extract, Top9, chili oil, sesame oil and Berna Star respectively. 2nd group contains on Bio-fly and (rosemary oil + sunflower oil) respectively
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