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Biochemical and Molecular Genetics Identification of Salicornia sp. and Sarcocornia sp. in the North Coast of Egypt
2020
Shams Hussein | ashraf Bakry | Laila Helmy | Nazmy Abdelghany
Because water scarcity anticipated to increase within the destiny in particular with growing global population and the rise in prosperity problem of the shortage of water suitable for cultivation of meals plants inside the global is growing in arid and semi-arid regions. There is the call to discover some other plant resource that doesn’t need freshwater i.e. able to grow using seawater. It is worth to note that Salicornia sp. and Sarcocornia sp. may be grown at the seawater. Currently, considered one of the most crucial issues dealing with Egypt is a way to provide food within the frame of limiting to be had soils for cultivation, limitation of water resources, especially after Ethiopian Nahda Dam and growing in population. Accordingly, the use of halophytes forage plants (Salicornia and Sarcocornia) using seawater has emerged as one in all the most exciting and intelligent research points. Therefore, a case observe was carried out in 2018 and 2019 to evaluate the nutritional status of Salicornia and Sarcocornia plants which can be grown on salty water in the North Coast of Egypt. Five samples of Salicornia and Sarcocornia amassed from Damietta Port Said coastal road and identified depends on phenotypic homes to Sarcocornia perennis (DA), Sarcocornia perennis (PS), Sarcocornia fruticosa, Salicornia europaea and Salicornia herbacea. Proximate composition analyses were carried out. It turned into obtrusive that, among dry biomass, carbohydrate has become in the most important proximate compositions in the Sarcocornia sp. and Salicornia sp. Tissues observed through ash. Molecular evaluation by SCoT techniques turned into done for Salicornia and Sarcocornia. The SCoT molecular marker techniques reach producing reproducible and dependable amplicons. Even though that the SCoT technique became higher in assessment for molecular variety and discrimination ability for all studied Salicornia and Sarcocornia.The results obtained have shown that the high nutritional value of the plant in terms of protein content, carbohydrates content and as a result, it is suitable for food
Show more [+] Less [-]ALLEVIATION OF SALINITY STRESS THROUGH MAGNETIC WATER AND NANO ZINC AND MAGNESIUM TREATMENT OF LEMONGRASS PLANT (Cymbopogen citratus L.)
2020
Ahmed Abdel Hamid
The effect of magnetic water compared with saline water , nano-Mg at 100 and 200 ppm and nano-Zn at 50 and 100 ppm treatments and their combinations on lemongrass plants (Cymbopogen citratus L.) was studied during 2018/2019 and 2019/2020.Vegetative growth parameters including plant height, number of tillers/ plant, leaf area, herb fresh and dry weights were greatly affected by both studied factors. Magnetic water treatments recorded the highest values of all vegetative growth parameters compared with saline water. However, all nanoparticles of Mg and Zn treatments were superior than control in promoting vegetative growth parameters and the treatments of 100 ppm nano-Zn and 200 ppm nano-Mg recorded the maximum values of vegetative growth parameters. The combined treatments showed that the highest values of vegetative growth measurements were obtained in plants irrigated with magnetic water and sprayed with 100 ppm nano-Zn, in both cuts In 1st and 2ndseasons.N, P, K and Mg contents were significantly higher in lemongrass plants irrigated with magnetic water than those irrigated with saline water. Moreover, the treatments of 100 ppm nano-Zn exhibited the highest significant values of N, P, K, whereas 100 and 200 ppm of nano-Mg treatments were superior in recording the highest values of Mg content, in the two seasons. The combination treatments of lemongrass plants irrigated with magnetic water and sprayed with 100 ppm nano-Zn or 200 ppm nano-Mg produced the richest values of N, P and K% D.W., whereas, the treatments of magnetic water combined with 100 or 200 ppm of nano-Mg produced the best Mg content.A great effect of magnetic water irrigation on increasing Fe and Zn contents than saline water treatments. The treatments of 50 and 100 ppm of nano-Zn were more effective than control and other treatments in maximizing Fe and Zn levels. The combined treatments of lemongrass plants irrigated with magnetic water and sprayed with 100 ppm of nano-Zn exhibited the highest values of Fe and Zn% D.W. in most cases. Additionally, total chlorophyll greatly increased in lemongrass plants irrigated with magnetic water than those irrigated with saline water, whereas the treatment of 200 ppm nano-Mg increased it than others. Interaction effect showed that, plants irrigated with magnetic water and sprayed with nano-Mg at 200 ppm exhibited the highest values of total chlorophyll.Proline and glycine betaine contents were greatly decreased with magnetic water treatment compared with saline water. Meanwhile, all applied treatments were effective in decreasing the levels of both constituents than control, which considered a good indicator to alleviation of water salinity stress on lemongrass plants through magnetic water and nanoparticles of Mg and Zn applications. Finally, volatile oil %, was greatly increased in lemongrass plants irrigated with magnetic water, especially the treatment of nano-Zn at 100 ppm as compared of others in this respect in both cuts and the two studied seasons. The interaction between the two studied factors was significant which means that both factors act together in improving volatile oil content in lemongrass herb
Show more [+] Less [-]Evaluation of mint and sweet basil herbs production integrated into the Aquaponic Tilapia production system
2020
Sabah Salama | Awad Kandil | Mohamed Elshenawy | Mohamed Abdelbaki | Mohamed Abulseoud
The impacts of climate change combined with water shortage and the need to increase food production to meet increasing population stimulate the implementation of Aquaponic systems not only on food scale but also on some medicinal plants in agriculture production. The current experiment was conducted at the Institute of Graduate Arid Land Agriculture and Research Institute (ALARI) at Ain Shams University, Egypt, to test sweet basil and mint development during the summer seasons of 2017 and 2018 under urban conditions through the aquaponic system. The objective of the study was to investigate the use of aquaponic compared to the chemical nutrient solution (control) as a nutritional source combined with two plant densities (6 and 8 plants / m²) in the design of complete randomized blocks for evaluating basil and mint growth. The density of the Nile Tilapia was 100 fish / m3 in the aquaponic facility. The yield characteristics, the nutrient (N, P and K) and the oil content (percent) of basil and mint, have been measured in. Reveled results indicated that basil plant had a higher capacity for removing NH4, NO3, P and K from fish rearing water than mint while plant density 8 plants/m2 had a higher capacity compared plant density 6 plants / m2 resulted enhancing the quality of fish rearing water led to increase the fish yield (the final and gain tilapia yield). The chemical nutrient solution provided higher plant height, fresh and dry yields of basil and mint than the aquaponic solution, as well as N, P, K, and oil content (percent) of basil and mint plants in both plant density (6 and 8 plants / m²) and in both seasons. The composition of aquaponic water as a source of nutrition was not sufficient to meet the requirements for basil and mint nutrients. The highest plant height, N, P, K, and oil content results were reported for the treatment of chemical nutrient solution + plant density 6 plants / m² while chemical nutrient solution + plant density 8 plants / m² had the highest fresh and dry yield of basil and mint. The need for increasing the food production under climate change impacts, the use of aquaponic systems for sweet basil and mint production provided food production (fish and vegetables) as well as the medicinal plants at the same time protecting the environment by avoiding the use of chemical fertilizers.
Show more [+] Less [-]Genetic Improvement of Calendula officinal through Mutation Induction using Gamma Irradiation and Chemical Mutagens
2020
eman elmenbawy | Eman Fahmy | Sawsan Elateek | Nahla Awad
Calendulais one of the basic plants of ornamentals. It is used in various purposes as cut flowers and in gardens. They are rich with active compounds such as chlorophyll a and b, phenols, flavonoid, and carotenoids. The present study focused on mutation induction in order to improve the morphology of the plant which can increase the turnout of its market. In order to examine which mutagen will make the most noticeable and permanent improvement of the plant. For the mutagens, seeds were treated with three doses of gamma ray (2500, 5000, 7000Gy) and two chemical mutagens; colchicine and EMS; which were applied at concentrations of 1000, 3000, and 10000 ppm. With the aim to measure the plant improvement, we measured the plant morphology, its active compounds and protein profiling before and after treatments with both γ-radiation and chemical mutagen to see which one has the most effective imprint on the plant. The morphological parameters data showed the highest plant high by the two doses; gamma dose of 7000 Gy and EMS concentration 10000 ppm, while the lowest dose of colchicine (1000 ppm) made the most impact on the plant height. As for the highest number of leaves/plant, it was obtained at 5000 Gy and 10000 ppm of EMS, while 1000 ppm of colchicine had the same effect. But the number of flowers/plant was not affected by the gamma radiation and colchicine but increased significantly by 10000 ppm of EMS. Surprise, the flower diameter was not affected by EMS while decreased at 2500 Gy and 3000 ppm of colchicine. Considering the importance of flower shape, all treatments either gamma radiation or chemical mutagens showed noticeable changes. Whereas, the biochemical parameters measuring the active compounds, both Chl-a, Chl-b and carotenoids contents increased at 5000 Gy, while flavonoid increased at 2500 Gy. Although, all doses of gamma ray eliminated the phenols content. While the lowest used concentration of colchicine (1000 ppm) increased both of Chl-a, Chl-b, flavonoid and carotenoids, even though the 10000 ppm of colchicine increased the phenol. On the other hand, the highest concentration of EMS (10000 ppm) increased Chl-a and Chl-b while carotenoids, flavonoid and phenol increased at 3000 ppm of EMS. The protein-profile analysis showed fluctuation in the gene expression. The highest performance appeared at 7000 Gy of gamma treatment,1000 -3000 ppm of colchicine, while the same result was obtained by EMS at 3000 ppm.
Show more [+] Less [-]دراسة اقتصادیة لبعض مزارع بط التسمین بمحافظة الشرقیة
2020
Ola Abdel-Megeed Mekhaimar Abd El-Maksoud | Mohamed Sentrisy | Fatma Abbas | Faisal Bayoumi
تعتبرصناعة الدواجن وخاصة تربیة البط من أحد الصناعات الزراعیة الهامة فى جمهوریة مصر العربیة ککل وبمحافظة الشرقیة بشکل خاص نظرا لترکز صناعة البط فى محافظة الشرقیة. لذا یهدف البحث الى دراسة الملامح الرئیسیة لصناعة انتاج البط فى محافظة الشرقیة بصفة خاصة. کما استهدف دراسة اقتصادیات هذه الصناعة بتقدیر دوال الانتاج. وحصر أهم المشاکل والمعوقات التى تواجه التوسع فى القطاع الانتاجى للبط. وتمثلت مشکلة البحث فی تقلص فى أعداد تربیة البط وعدم اهتمام معظم المربیین بالمزارع وعدم توفر آلیة واضحة من قبل الدولة للاهتمام بقطاع انتاج البط بالجمهوریة ککل وبمحافظة الشرقیة بشکل خاص بالرغم من الممیزات العدیدة لتربیة البط ومعامل تحویله الغذائى العالى ومقاومته العالیة للأمراض، الأمر الذى دفع بالباحث الى دراسة اقتصادیات بعض المزارع المتخصصة بتربیة البط بالمحافظة، وحصر أهم المشاکل والمعوقات التى تواجه أصحاب هذه المزارع. أعتمدت الدراسة على استخدام أسلوب التحلیل الوصفى والکمى وأسالیب الانحدار البسیط والمتعدد. کما اعتمدت الدراسة علی البیانات الثانویة بمدیریة الزراعة بمحافظة الشرقیة، بالإضافة إلى بیانات أولیة جمعت بواسطة استمارة استبیان. وکانت اهم النتائج:ان اکثر المشاکل الانتاجیة المتعلقة بالکتاکیت، تمثلت فی ارتفاع أسعار الکتاکیت وعدم جودتها وعدم توافر مصدر شراء الکتکوت، بینما المشاکل الانتاجیة المتعلقة بالأعلاف کان أرتفاع أسعارها وعدم تعامل الشرکة مع المربى مباشرة وعدم جودته وسوء التخزین وشراء اعلاف مجهولة المصدر وعدم توافر مصدر شراء العلف، بالاضافة الى المشاکل المتعلقة بالادویة والخدمات البیطریة وتمثلت فی مشکلة ارتفاع اسعار الادویة وعدم وجود الادویة وعدم توافر الاشراف البیطری، هذا بالاضافة الى المشاکل المتعلقة بالعمالة والمتمثلة فی قلة العمالة المدربة وارتفاع الاجر, وهناک مشاکل اخرى مثل مشکلة عدم توافر مصادر الوقود وعدم توافر مصادر الفرشة. کما تبین من دالة الناتج الکلی (Q) أن أهم العناصر الانتاجیة ذات التأثیر المعنوی علی الناتج الکلی للدورة الواحدة من مزارع بط التسمین تتمثل فى کمیة العلیقة المقدمة وعدد العمال وعدد الکتاکیت فی اغلب السعات الانتاجیة.وأوصى البحث بما یلى : 1. العمل على نشر ثقافة التدریب وذلک لتوفیر العمالة الفنیة المدربة.2. توفیر حملات توعیة بیطریة , وتوفیر أمصال ولقحات فعالة.3. توفیر الأعلاف باسعار مناسبة.
Show more [+] Less [-]INFLUENCE OF DIETARY THREONINE AND TRYPTOPHAN SUPPLEMENTATIONS ON BROILER PRODUCTIVITY TRAITS UNDER EGYPTIAN SUMMER CONDITIONS
2020
Fathy Abdel_Azeem | Amer Helal | Hany Thabel | Ahmed Naguib Elbaz
A study was conducted to investigate the effects of feeding different levels of threonine and tryptophan on growth performance, some serum parameters and carcass traits of broiler chicks under Egyptian summer conditions. Three hundred and sixty Ross 308, one-day-old broiler chicks were randomly allocated into nine treatments with 4 replicates of 10 chicks each. Three levels of threonine (100, 150 or 200 %) and three levels of tryptophan (100, 150 or 200%) according to dietary requirements for Ross 308 chick's guidebook were used in a factorial design arrangement to obtain nine experimental treatments. Two periodical diets were used (starter phase 1-21 days and finisher phase 22-35 days). Growth performance traits including live body weight (LBW), Body weight gain (BWG), feed consumption (FC) and feed conversion ratio (FCR) were recorded at the end of each week. Carcasses were manually eviscerated and weighed. Components of total protein, albumin, cholesterol, and triglycerides were analyzed by using commercial kits. The results showed that chicks fed diet containing 200 % threonine had an improvement in live body weight, body weight gain and feed conversion ratio. The performance index increased with the addition of threonine and tryptophan in the broiler diets. Serum total protein and globulin concentrations were affected by supplemental threonine and tryptophan. Dietary threonine, tryptophan did not affect serum albumin and triglycerides. Total cholesterol was increased with supplementation of threonine, tryptophan and interaction between them. Chicks fed 100% dietary threonine had the significantly better results in terms of the relative weights of carcass, edible parts and bursa compared to the chicks fed other dietary threonine levels, although tryptophan amino acid had not any significant effect on the above-mentioned traits. Giblets, liver, and heart weights percentage increase with increasing tryptophan level in the broiler diets. The relative weight of gizzard reduced with increasing level of both amino acids in the diets.In this study, it concluded that supplementation of threonine and tryptophan improves productive performance and health status of broiler chicks without imposing any toxicity as all blood components were in normal range.
Show more [+] Less [-]A TRIAL TO DETECT THE EFFECT OF STRAIN AND DIFFERENT SOURCES OF DRINKING WATER ON SOME PRODUCTIVE TRAITS IN BROILER CHICKS
2019
Shimaa Shaker | S. El-Safty | U. Shoureap | Zienb El- Awamry
The main goal of this experiment was to evaluate the effect of broiler strain and source of drinking water on some performance traits (body weight and carcass traits). Sample of water were taken from two different location sources, Giza governorate (as Nile water) and Qalubia governorate (as well water or ground water). Three different broiler strains were used in this study (Arbor acres, Hubbard and Cobb). Lead concentration (as a heavy metal element) and bacteriological analysis (total bacteria and fecal coliforms) in both water and carcass were carried out. The main results obtained can be summarized as follow: Arbor acres strain recorded the heaviest body at most ages compared to the other strains, the difference was highly significant at 5 and 6 weeks of ages. Total bacteria were significantly high in ground water compared to Nile water. Conversely, Coliforms bacteria count was significantly high in Nile water compared to ground water source. Regardless strain type, high positive correlation between lead concentration in drinking water and lead concentration in liver, kidney and carcass weight was observed
Show more [+] Less [-]DETECTION FOR MECA GENE IN EGYPTIAN CLINICAL Staphylococcus aureus SAMPLES AND STUDY THEIR GENOTOXIC ACTIVITY
2019
Marwa Hassan Hassan | S. Ibrahim | A. Abdel Razek | Sawsan Elateek
Staphylococcus aureus isolates were isolated from fifty clinical samples collected from Ain Shams University Hospitals (March - July 2016) and identified using biochemical and microbiological tests. PCR was performed using specific primers to determine the isolates of Methicillin - Resistant Staphylococcus aureus (MRSA) based on the presence of mecA gene. Thirty-four isolates from total fifty isolates (68%) were identified as MRSA isolates. To study the genotoxicity for this isolates, forty-five mice were injected with these MRSA isolates and comet and micronuclei assays were performed on mice liver tissues and bone marrow respectively. These assays revealed 24% - 22% DNA damage as an indicator for chromosomal breakage by comet and micronucleus assays respectively which indicate that infection with these isolates leads to mutations. Studying these isolate furthermore will give an insight on how critical maintaining high standard hygiene in Egyptian hospitals and attention to infection control system can prevent occur of outbreaks
Show more [+] Less [-]EFFECT OF FOLIAR APPLICATION WITH ALGAE AND MORINGA LEAVES EXTRACTS ON VEGETATIVE GROWTH, LEAF MINERAL CONTENTS, YIELD AND CHEMICAL FRUIT QUALITY OF PICUAL OLIVE TREES
2019
A. Hassan | N. Abd-Alhamid | Rawheya Aly | H. Hassan
This study was carried out during 2017 and 2018 seasons on adult Picual olive cv. (8 years old) in a private orchard located at Cairo Ismailia Desert Road, (about 80 Km from Cairo), Ismailia Governorate, Egypt. Trees are grown in sandy soil, under drip irrigation system, uniform in shape and received the common horticultural practices, therefore, this work aims to study the effect of spraying different concentrations and dates spray of algae and moringa leaf extract (MLE) for tow times on the vegetative growth, leaf mineral contents, yield and fruit chemical properties of Picual olive trees. Results indicated that all different spraying treatments of algae and moringa extract at different spraying dates had a positive effect on vegetative growth, leaf mineral contents, fruit set, yield and fruit chemical properties in comparison with the control. Treatments of algae extract at 0.4% + moringa leaves extract at 2% and algae extract at 0.4% + moringa leaves extract at 4% were the most effective treatments to increase vegetative growth, improve leaf mineral content, fruit set, yield and oil content. Thus, these treatments can be recommended for commercial use under similar conditions
Show more [+] Less [-]EFFECT OF MYCORRHIZAE AND DIFFERENT RATES OF NPK ON VEGETATIVE GROWTH AND YIELD OF TOMATO PLANT UNDER NORTH SINAI CONDITIONS
2019
M. Hegazy | A. Abou El-Yazied | H. Abd El-Gawad | A. Abd-Elbaset
The experiments were conducted to evaluate the effect of mycorrhizae (Glomus sp. and Gigspora sp.) application on the vegetative growth and yield of tomato plants (Alisa cultivar), and its relevance to the mineral fertilizers requirements as NPK during the seasons of 2015/2016 and 2016/2017. The experiments were conducted at Baloza Research Station, Desert Research Center, North Sinai Governorate, Egypt. The experimental design was a randomized complete block design with three replicates, every replicate included 4 treatments (75 and100% of the recommended mineral fertilizer only or combined with the mycorrhizae treatment). The results showed that mycorrhizae application significantly increased the vegetative growth characters of tomato plant such as plant height, number of shoots, number of leaves, stem diameter, fresh and dry weights and yield. There were no significant differences in the vegetative growth characteristics of tomato plants receiving 75% of mineral fertilizers in addition to mycorrhizae application as compared with plants receiving 100% of mineral fertilizers only, except for the fresh weight of plants. The plants were received 100% of the recommended mineral fertilizers combined with mycorrhizae treatment gave the highest values of vegetative growth characters and yield. Therefore, we recommended that, using of mycorrhizae could be affective to maximize the utilization of mineral fertilizers, in addition, the mycorrhizea is ecofriendly, economically and consid ered as one of the most important bio-fertilizer resources.
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