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Inhibition of Rhizoctonia solani Growth and Its Extracellular Hydrolytic Enzymes by Different Extracts of Cinnamon (Cinnamomum cassia) and Black Cumin Seeds (Nigella sativa) Full text
2022
Abd El Aziz, Seham | Abo-shady, Ahmed | Ibrahim, Mervat | Helmy, Maha
Inhibition of Rhizoctonia solani Growth and Its Extracellular Hydrolytic Enzymes by Different Extracts of Cinnamon (Cinnamomum cassia) and Black Cumin Seeds (Nigella sativa) Full text
2022
Abd El Aziz, Seham | Abo-shady, Ahmed | Ibrahim, Mervat | Helmy, Maha
The present study evaluated the ability of different extracts of cinnamon and black cumin seeds to inhibit the growth of the phytopathogenic fungus Rhizoctonia solani and its extracellular cell wall degrading enzymes. Concentrations of 300 and 450 ppm of methylene chloride and hexane extracts of cinnamon completely inhibited the growth of R. solani after 72 hours of incubation at 25±1°C in vitro. Methylene chloride or hexane extracts of black cumin seeds at 4000 ppm after 72 hours inhibited the growth of R. solani by 37 and 39% respectively. Moreover, black cumin seed hexane extract at 2000 ppm inhibited 55% and 38% of the activity of pectin lyase (PL) and polygalacturonase (PG) respectively. In addition, the methanolic extract of black cumin seeds at 2000 ppm exhibited a significant reduction of exo-protease activity (74.8%). GC-MS analysis results showed that linoleic acid is the main component of the fixed oil fraction of black cumin seed hexane extract while (E) cinnamaldehyde is the main component in both hexane and methylene chloride extracts of cinnamon. HPLC-MS analysis of black cumin seeds methanolic extract showed that amentoflavone was the main component. Docking was used to identify the major component interaction with pectin lyase A and exo-protease.
Show more [+] Less [-]Inhibition of Rhizoctonia solani Growth and Its Extracellular Hydrolytic Enzymes by Different Extracts of Cinnamon (Cinnamomum cassia) and Black Cumin Seeds (Nigella sativa) Full text
2022
Seham Abd El Aziz | Ahmed Abo-shady | Mervat Ibrahim | Maha Helmy
The present study evaluated the ability of different extracts of cinnamon and black cumin seeds to inhibit the growth of the phytopathogenic fungus Rhizoctonia solani and its extracellular cell wall degrading enzymes. Concentrations of 300 and 450 ppm of methylene chloride and hexane extracts of cinnamon completely inhibited the growth of R. solani after 72 hours of incubation at 25±1°C in vitro. Methylene chloride or hexane extracts of black cumin seeds at 4000 ppm after 72 hours inhibited the growth of R. solani by 37 and 39% respectively. Moreover, black cumin seed hexane extract at 2000 ppm inhibited 55% and 38% of the activity of pectin lyase (PL) and polygalacturonase (PG) respectively. In addition, the methanolic extract of black cumin seeds at 2000 ppm exhibited a significant reduction of exo-protease activity (74.8%). GC-MS analysis results showed that linoleic acid is the main component of the fixed oil fraction of black cumin seed hexane extract while (E) cinnamaldehyde is the main component in both hexane and methylene chloride extracts of cinnamon. HPLC-MS analysis of black cumin seeds methanolic extract showed that amentoflavone was the main component. Docking was used to identify the major component interaction with pectin lyase A and exo-protease.
Show more [+] Less [-]Improvement of Growth and Productivity of Mango Trees Using Some Growth Stimulants under Salinity Conditions Full text
2022
Abdalla, Fatma | Elwakeel, Hassan | Mansour, Noha | Osman, Sabry
Improvement of Growth and Productivity of Mango Trees Using Some Growth Stimulants under Salinity Conditions Full text
2022
Abdalla, Fatma | Elwakeel, Hassan | Mansour, Noha | Osman, Sabry
A study was carried out in a mango orchard on reclaimed land for two seasons in 2019 and 2020. The experiment was implemented on fifteen years old mango trees “Fagri Kalan” budded on seedling mango rootstock and planted at 4 × 4 m. This experiment involved two soil growth stimulants (K humate, magnetite) and foliar application with cobalt. The experiment involved two factors, the first one was soil application with K humate by two levels (50 and 100 g/tree/year) and magnetite by two levels (250 and 500 g/tree /year) plus control, whereas the second factor was a foliar application with Co as cobalt sulfate by three levels (0, 15, and 30 ppm Co). The experiment was carried out in a factorial experiment in split plot design with three replicates whereas each replicate was represented by two trees. Result indicated that soil application with K humate at 100 g/tree followed by magnetite at 250 g/tree or foliar application with Co at 15 ppm alone or the combination between (K humate at 100 g/tree and cobalt at 15 ppm) gave the highest values of yield/tree, fruit weight, TSS, reducing sugars, leaf N, P, K, and Fe content of “Fagri Kalan” mango trees grown under salinity conditions.
Show more [+] Less [-]Improvement of Growth and Productivity of Mango Trees Using Some Growth Stimulants under Salinity Conditions Full text
2022
Fatma Abdalla | Hassan Elwakeel | Noha Mansour | Sabry Osman
A study was carried out in a mango orchard on reclaimed land for two seasons in 2019 and 2020. The experiment was implemented on fifteen years old mango trees “Fagri Kalan” budded on seedling mango rootstock and planted at 4 × 4 m. This experiment involved two soil growth stimulants (K humate, magnetite) and foliar application with cobalt. The experiment involved two factors, the first one was soil application with K humate by two levels (50 and 100 g/tree/year) and magnetite by two levels (250 and 500 g/tree /year) plus control, whereas the second factor was a foliar application with Co as cobalt sulfate by three levels (0, 15, and 30 ppm Co). The experiment was carried out in a factorial experiment in split plot design with three replicates whereas each replicate was represented by two trees. Result indicated that soil application with K humate at 100 g/tree followed by magnetite at 250 g/tree or foliar application with Co at 15 ppm alone or the combination between (K humate at 100 g/tree and cobalt at 15 ppm) gave the highest values of yield/tree, fruit weight, TSS, reducing sugars, leaf N, P, K, and Fe content of “Fagri Kalan” mango trees grown under salinity conditions.
Show more [+] Less [-]Element Composition and Toxicity of Seaweed Algae (Ulva lactuca, Laurencia obtuse and Padina pavonica) Toward Shrimp Full text
2022
Muhammad, Ahmed | Hassan, Ibrahim | Tawfeek, Manar | Ahmed Ibrahim, Hayam
Element Composition and Toxicity of Seaweed Algae (Ulva lactuca, Laurencia obtuse and Padina pavonica) Toward Shrimp Full text
2022
Muhammad, Ahmed | Hassan, Ibrahim | Tawfeek, Manar | Ahmed Ibrahim, Hayam
This study examined the macro and trace element contents as well as the cytotoxicity of Laurencia obtusa, Ulva lactuca, and Padina pavonica algae collected from the Mediterranean coast of Egypt (Alexandria coast). The results indicated that the seaweed samples contained high concentrations of Ca, Cl, Mg, K, Na, P and Fe. The Cu and Zn contents of the algae were in the ranges of 0.39-0.95 mg/100 g dw and 0.11-0.49 mg/100 g dw respectively. Fresh red algae contained the highest level of Zn and Cu, whereas the iodine level was 1.52, 1.27 and 1.01 mg/100g in fresh each of Padina pavonica, Laurencia obtuse and Ulva lactuca respectively. Fresh red, fresh green, dry green, dry red, in addition to fresh and dried brown algae had maximum lead (Pb) levels of 0.16, 0.14, 0.13, 0.12, 0.04 and 0.02 g/100 g respectively. Fresh red algae contained the highest Cd and Mn levels, whereas dried brown algae exhibited the lowest Cd and Mn concentrations. Different extracts of fresh and dried brown algae exhibited significant larvicidal activity against brine shrimp. LC50 values indicated that dry samples were more cytotoxic than fresh samples.
Show more [+] Less [-]Element Composition and Toxicity of Seaweed Algae (Ulva lactuca, Laurencia obtuse and Padina pavonica) Toward Shrimp Full text
2022
Ahmed Muhammad | Ibrahim Hassan | Manar Tawfeek | Hayam Ahmed Ibrahim
This study examined the macro and trace element contents as well as the cytotoxicity of Laurencia obtusa, Ulva lactuca, and Padina pavonica algae collected from the Mediterranean coast of Egypt (Alexandria coast). The results indicated that the seaweed samples contained high concentrations of Ca, Cl, Mg, K, Na, P and Fe. The Cu and Zn contents of the algae were in the ranges of 0.39-0.95 mg/100 g dw and 0.11-0.49 mg/100 g dw respectively. Fresh red algae contained the highest level of Zn and Cu, whereas the iodine level was 1.52, 1.27 and 1.01 mg/100g in fresh each of Padina pavonica, Laurencia obtuse and Ulva lactuca respectively. Fresh red, fresh green, dry green, dry red, in addition to fresh and dried brown algae had maximum lead (Pb) levels of 0.16, 0.14, 0.13, 0.12, 0.04 and 0.02 g/100 g respectively. Fresh red algae contained the highest Cd and Mn levels, whereas dried brown algae exhibited the lowest Cd and Mn concentrations. Different extracts of fresh and dried brown algae exhibited significant larvicidal activity against brine shrimp. LC50 values indicated that dry samples were more cytotoxic than fresh samples.
Show more [+] Less [-]Effect of Variable Frequency Drive and Pipe Materials on Guava Juice Flow Full text
2022
Shalaby, Shereen | Mustafa, Mubarak | Heikal, Yahiya | El Attar, Mahmoud
Effect of Variable Frequency Drive and Pipe Materials on Guava Juice Flow Full text
2022
Shalaby, Shereen | Mustafa, Mubarak | Heikal, Yahiya | El Attar, Mahmoud
In this study, a designed variable frequency drive (VFD) was used to control a single-phase induction motor’s speed to pump guava juice at two solid concentrations (9°Bx and 11°Bx). Next, the pressure drop was measured in stainless-steel pipes of three diameters. The pressure drop percentage difference before and after using the VFD at varied flow rates with 10 repetitions at 5-minute intervals was evaluated. The pressure drop reduction range was 19.7%– 30.8% and 19.2 %–32.4% for the 9% and 11% solid concentrations respectively after using the VFD, which resulted in an average of 25.73% reductions in pressure drop and the pump’s total head and driving power. The rheological properties of guava juice were investigated at various temperatures (5, 15, 25, 35, 55 and 75°C). From the results, all materials exhibited non-Newtonian pseudoplastic behavior at all temperatures and concentrations, and correlated well with the power law model, with flow behavior index (n) values less than unity for the 9% and 11% solid concentrations. The findings offer helpful information for predicting how heat variations during processing influence the behavior of guava juice concentrates.
Show more [+] Less [-]Effect of Variable Frequency Drive and Pipe Materials on Guava Juice Flow Full text
2022
Shereen Shalaby | Mubarak Mustafa | Yahiya Heikal | Mahmoud El Attar
In this study, a designed variable frequency drive (VFD) was used to control a single-phase induction motor’s speed to pump guava juice at two solid concentrations (9°Bx and 11°Bx). Next, the pressure drop was measured in stainless-steel pipes of three diameters. The pressure drop percentage difference before and after using the VFD at varied flow rates with 10 repetitions at 5-minute intervals was evaluated. The pressure drop reduction range was 19.7%– 30.8% and 19.2 %–32.4% for the 9% and 11% solid concentrations respectively after using the VFD, which resulted in an average of 25.73% reductions in pressure drop and the pump’s total head and driving power. The rheological properties of guava juice were investigated at various temperatures (5, 15, 25, 35, 55 and 75°C). From the results, all materials exhibited non-Newtonian pseudoplastic behavior at all temperatures and concentrations, and correlated well with the power law model, with flow behavior index (n) values less than unity for the 9% and 11% solid concentrations. The findings offer helpful information for predicting how heat variations during processing influence the behavior of guava juice concentrates.
Show more [+] Less [-]Effect of Different Levels of Salinity and Anti-Transpiration on the Growth Characteristics and Chemical Composition of Panicum maximum (Jacq.) Full text
2022
El Wardany, Adel | Elbordiny, Nasr | Abdrabou, Ramadan | Bakr, Adel | Abdelkareem, Yasser
Effect of Different Levels of Salinity and Anti-Transpiration on the Growth Characteristics and Chemical Composition of Panicum maximum (Jacq.) Full text
2022
El Wardany, Adel | Elbordiny, Nasr | Abdrabou, Ramadan | Bakr, Adel | Abdelkareem, Yasser
This study aimed to determine the effect of different salinity and anti-transpiration levels on the growth and biochemical composition of Panicum maximum plants (Guinea grass) during the spring and summer of 2020. Two different anti-transpiration treatments [molasses (sugarcane) (5 mL/L), kaolin (50 g/L) and control] and three salinity levels (S1 well water as the control and salinity S2 2000 ppm; S3 4000 ppm, S4 6000 ppm) were used in 12 treatments in 72 pots (3 anti-transpiration treatments × 4 salinity treatments × 6 replicates); a randomized complete design was used. Results revealed that the plants achieved the highest plant height (123.77 cm) and dry weight (521.87 g/m2) with kaolin and no salt addition treatment during summer. A higher percentage of proteins and carbohydrates were found in spring than in summer, but there was no significant difference in the salinity levels. A high percentage of ash and fiber contents was also observed during summer, with no significant differences between the anti-transpiration treatments. It could be concluded that P. maximum, as one of the most important fodder crops, could be cultivated in marginal lands, especially during the summer season.
Show more [+] Less [-]Effect of Different Levels of Salinity and Anti-Transpiration on the Growth Characteristics and Chemical Composition of Panicum maximum (Jacq.) Full text
2022
Adel El Wardany | Nasr Elbordiny | Ramadan Abdrabou | Adel Bakr | Yasser Abdelkareem
This study aimed to determine the effect of different salinity and anti-transpiration levels on the growth and biochemical composition of Panicum maximum plants (Guinea grass) during the spring and summer of 2020. Two different anti-transpiration treatments [molasses (sugarcane) (5 mL/L), kaolin (50 g/L) and control] and three salinity levels (S1 well water as the control and salinity S2 2000 ppm; S3 4000 ppm, S4 6000 ppm) were used in 12 treatments in 72 pots (3 anti-transpiration treatments × 4 salinity treatments × 6 replicates); a randomized complete design was used. Results revealed that the plants achieved the highest plant height (123.77 cm) and dry weight (521.87 g/m2) with kaolin and no salt addition treatment during summer. A higher percentage of proteins and carbohydrates were found in spring than in summer, but there was no significant difference in the salinity levels. A high percentage of ash and fiber contents was also observed during summer, with no significant differences between the anti-transpiration treatments. It could be concluded that P. maximum, as one of the most important fodder crops, could be cultivated in marginal lands, especially during the summer season.
Show more [+] Less [-]Effects of Irrigation Systems on Sugar Beet Yield Full text
2022
Kassem, Alaa | El-Gindy, Abdel-Ghany | Hassan, Ahmed
Effects of Irrigation Systems on Sugar Beet Yield Full text
2022
Kassem, Alaa | El-Gindy, Abdel-Ghany | Hassan, Ahmed
The combined data from two growing seasons in a field experiment study by using two irrigation systems (surface and subsurface drip) in terms of additional water for sugar beet plants under saline conditions are used to estimate root and sugar yield, water use efficiency and root penetration power at the time of harvest. Water salinity levels of 6000 and 8000 ppm were applied to irrigate sugar beet from the time of planting on October 3 to the time of harvest on April 22 with three additions of water (limited 1750, moderate 2500 and optimum 3250 m3/fed). The results are summarized as follows: The heaviest root and highest sugar yield as well as highest water use efficiency were recorded when using subsurface irrigation system, 6000 ppm water salinity level, and moderate or optimum irrigation water (2500 or 3250 m3/fed). Oppositely, the highest penetration power values were obtained using drip irrigation systems with low water quantity (1750 m3/fed) and high-water salinity level (8000 ppm).
Show more [+] Less [-]Effects of Irrigation Systems on Sugar Beet Yield Full text
2022
Alaa Kassem | Abdel-Ghany El-Gindy | Ahmed Hassan
The combined data from two growing seasons in a field experiment study by using two irrigation systems (surface and subsurface drip) in terms of additional water for sugar beet plants under saline conditions are used to estimate root and sugar yield, water use efficiency and root penetration power at the time of harvest. Water salinity levels of 6000 and 8000 ppm were applied to irrigate sugar beet from the time of planting on October 3 to the time of harvest on April 22 with three additions of water (limited 1750, moderate 2500 and optimum 3250 m3/fed). The results are summarized as follows: The heaviest root and highest sugar yield as well as highest water use efficiency were recorded when using subsurface irrigation system, 6000 ppm water salinity level, and moderate or optimum irrigation water (2500 or 3250 m3/fed). Oppositely, the highest penetration power values were obtained using drip irrigation systems with low water quantity (1750 m3/fed) and high-water salinity level (8000 ppm).
Show more [+] Less [-]Effect of Salinity on Seed Germination, Growth and Amino Acid Content in Fenugreek (Trigonella faenum-graecum L) Sprouts Full text
2022
Elgebaly, Atef | Sadek, Engy | Taha, Noura | Abou Hadid, Ayman
Effect of Salinity on Seed Germination, Growth and Amino Acid Content in Fenugreek (Trigonella faenum-graecum L) Sprouts Full text
2022
Elgebaly, Atef | Sadek, Engy | Taha, Noura | Abou Hadid, Ayman
This study was conducted to evaluate the effect of salt stress on amino acid profile and proximate analysis of fenugreek germination samples of two varieties: Giza 2 and Giza 30 for three days. The germination of sterilized fenugreek seeds was conducted using tap water and NaCl solution (2,000 ppm) where higher salt concentration lowered fenugreek germination quality. The germination of fenugreek seeds using tap water or brine increased the crude protein in both dry Giza 2 and Giza 30 seed varieties. Furthermore, sedative sprouts of Giza 30 recorded the highest crude protein (30.60%), fat (6.20%) and energy (333.13 kcal/g) while the use of sterilized Giza 2 seed variety recorded a higher percentage of carbohydrates (43.3%), fiber (8.10%) and ash (6.60%). Alternatively, although amino acid profiles indicated that aspartic acid and proline were most abundant in fenugreek sprouts, sulfur amino acids (cysteine and methionine) demonstrated the least values of the two varieties. Adopting saline water in germinating fenugreek seeds for the two Giza 2 and Giza 30 varieties decreased all amino acid contents compared with those of tap water treatment.
Show more [+] Less [-]Effect of Salinity on Seed Germination, Growth and Amino Acid Content in Fenugreek (Trigonella faenum-graecum L) Sprouts Full text
2022
Atef Elgebaly | Engy Sadek | Noura Taha | Ayman Abou Hadid
This study was conducted to evaluate the effect of salt stress on amino acid profile and proximate analysis of fenugreek germination samples of two varieties: Giza 2 and Giza 30 for three days. The germination of sterilized fenugreek seeds was conducted using tap water and NaCl solution (2,000 ppm) where higher salt concentration lowered fenugreek germination quality. The germination of fenugreek seeds using tap water or brine increased the crude protein in both dry Giza 2 and Giza 30 seed varieties. Furthermore, sedative sprouts of Giza 30 recorded the highest crude protein (30.60%), fat (6.20%) and energy (333.13 kcal/g) while the use of sterilized Giza 2 seed variety recorded a higher percentage of carbohydrates (43.3%), fiber (8.10%) and ash (6.60%). Alternatively, although amino acid profiles indicated that aspartic acid and proline were most abundant in fenugreek sprouts, sulfur amino acids (cysteine and methionine) demonstrated the least values of the two varieties. Adopting saline water in germinating fenugreek seeds for the two Giza 2 and Giza 30 varieties decreased all amino acid contents compared with those of tap water treatment.
Show more [+] Less [-]Evaluation of Orius laevigatus as a predator of Bemisia tabaci In Vitro Full text
2022
Salama, Nada | Emam, Azza | washahy, Kadry | Gaffar, Saad
Evaluation of Orius laevigatus as a predator of Bemisia tabaci In Vitro Full text
2022
Salama, Nada | Emam, Azza | washahy, Kadry | Gaffar, Saad
This study determined the ability of Orius laevigatus to consume different stages of Bemisia tabaci at various densities. Additionally, the use of B. tabaci as food and its effect on the life cycle parameters and female fecundity of Orius predator developmental stages were investigated. The results showed that O. laevigatus could consume whitefly at various stages and complete its preimaginal and postimaginal stages, with B. tabaci eggs, nymph instars, and adults as the only available food source. The consumption and fecundity of O. laevigatus were influenced by feeding on different stages of B. tabaci. Furthermore, the females of this predator consumed more eggs, fourth nymphal instar, and adults than the first, second, and third instar nymphs of B. tabaci on a different density level. The mean numbers of consumptions at different stages of B. tabaci increased with increasing prey densities. The result simply that O. laevigatus can be used as a predator in the biological control of tobacco whiteflies.
Show more [+] Less [-]Evaluation of Orius laevigatus as a predator of Bemisia tabaci In Vitro Full text
2022
Nada Salama | Azza Emam | Kadry washahy | Saad Gaffar
This study determined the ability of Orius laevigatus to consume different stages of Bemisia tabaci at various densities. Additionally, the use of B. tabaci as food and its effect on the life cycle parameters and female fecundity of Orius predator developmental stages were investigated. The results showed that O. laevigatus could consume whitefly at various stages and complete its preimaginal and postimaginal stages, with B. tabaci eggs, nymph instars, and adults as the only available food source. The consumption and fecundity of O. laevigatus were influenced by feeding on different stages of B. tabaci. Furthermore, the females of this predator consumed more eggs, fourth nymphal instar, and adults than the first, second, and third instar nymphs of B. tabaci on a different density level. The mean numbers of consumptions at different stages of B. tabaci increased with increasing prey densities. The result simply that O. laevigatus can be used as a predator in the biological control of tobacco whiteflies.
Show more [+] Less [-]Evaluation of Inbred Lines of Maize in a Diallel Cross under Normal Condition and Drought Stress Full text
2022
Abd-Elnaser, Muhammed | Fazaa, Mostafa | Saleh, Samir | Abdelsalam, Mohamed | Shawky, Mahmoud
Evaluation of Inbred Lines of Maize in a Diallel Cross under Normal Condition and Drought Stress Full text
2022
Abd-Elnaser, Muhammed | Fazaa, Mostafa | Saleh, Samir | Abdelsalam, Mohamed | Shawky, Mahmoud
In this study, we examined eight white inbred lines of maize and their F1 crosses under normal and drought stress conditions to estimate their combining ability for grain yield and associated traits. The results showed significant correlation (mean squares) of irrigation treatment with the studied traits. The effects of parents, crosses, and genotypes were all determined to be highly significant under both irrigation levels. Grain yield and other traits showed significant differences (mean squares) associated with both General combining ability (GCA) and Specific combining ability (SCA) under both irrigation regimes, demonstrating the importance of both additive and nonadditive genetic effects in the expression of performance traits. The parental line (P-86) had positive and highly significant GCA effects, as well as the crosses (P-17×P-96), (P-8×P-96), (P-8×P-171), (P-24×P-86), (P-86×P-96), (P-86×P-171), and (P-96×P-171) which then gave the highest specific combinations under both irrigation regimes for grain yield and some of the associated traits. The highest level of heterosis (heterobeltiosis) for grain yield was obtained in the crosses (P-8×P-96), (P-8×P-137), (P-8×P-171), (P-96×P137), and (P-96×P-171) under both irrigation regimes.
Show more [+] Less [-]Evaluation of Inbred Lines of Maize in a Diallel Cross under Normal Condition and Drought Stress Full text
2022
Muhammed Abd-Elnaser | Mostafa Fazaa | Samir Saleh | Mohamed Abdelsalam | Mahmoud Shawky
In this study, we examined eight white inbred lines of maize and their F1 crosses under normal and drought stress conditions to estimate their combining ability for grain yield and associated traits. The results showed significant correlation (mean squares) of irrigation treatment with the studied traits. The effects of parents, crosses, and genotypes were all determined to be highly significant under both irrigation levels. Grain yield and other traits showed significant differences (mean squares) associated with both General combining ability (GCA) and Specific combining ability (SCA) under both irrigation regimes, demonstrating the importance of both additive and nonadditive genetic effects in the expression of performance traits. The parental line (P-86) had positive and highly significant GCA effects, as well as the crosses (P-17×P-96), (P-8×P-96), (P-8×P-171), (P-24×P-86), (P-86×P-96), (P-86×P-171), and (P-96×P-171) which then gave the highest specific combinations under both irrigation regimes for grain yield and some of the associated traits. The highest level of heterosis (heterobeltiosis) for grain yield was obtained in the crosses (P-8×P-96), (P-8×P-137), (P-8×P-171), (P-96×P137), and (P-96×P-171) under both irrigation regimes.
Show more [+] Less [-]DNA Barcoding for Some Medicinal Plants in the El-Omayed Biosphere Reserve Full text
2022
Nabil, Reham | Rashed, Mohamed | Elmosallamy, Mahmoud | Elsherbeny, Esraa
DNA Barcoding for Some Medicinal Plants in the El-Omayed Biosphere Reserve Full text
2022
Nabil, Reham | Rashed, Mohamed | Elmosallamy, Mahmoud | Elsherbeny, Esraa
DNA barcoding is a valuable tool for molecular identification of plant materials, which is primarily employed to guarantee the nature and therapeutic value of all available medicinal plants. Identification is crucial for the illegal medicinal plant trade, safe use, and preventing adulteration. In this study, DNA barcoding was used to evaluate and confirm the identification of some medicinal plants from the El-Omayed Biosphere Reserve (OBR). Two DNA regions, rbcL and ITS were selected due to their universality, ease of amplification and sequencing, and ability to identify taxa at the generic and species levels. The ITS region demonstrated a higher capacity for species discrimination power than the rbcL region. It assisted in identifying four of eight plants more precisely than rbcL. Together, ITS and rbcL could be used for plant species identification, conservation, and trade control of valuable plant resources.
Show more [+] Less [-]DNA Barcoding for Some Medicinal Plants in the El-Omayed Biosphere Reserve Full text
2022
Reham Nabil | Mohamed Rashed | Mahmoud Elmosallamy | Esraa Elsherbeny
DNA barcoding is a valuable tool for molecular identification of plant materials, which is primarily employed to guarantee the nature and therapeutic value of all available medicinal plants. Identification is crucial for the illegal medicinal plant trade, safe use, and preventing adulteration. In this study, DNA barcoding was used to evaluate and confirm the identification of some medicinal plants from the El-Omayed Biosphere Reserve (OBR). Two DNA regions, rbcL and ITS were selected due to their universality, ease of amplification and sequencing, and ability to identify taxa at the generic and species levels. The ITS region demonstrated a higher capacity for species discrimination power than the rbcL region. It assisted in identifying four of eight plants more precisely than rbcL. Together, ITS and rbcL could be used for plant species identification, conservation, and trade control of valuable plant resources.
Show more [+] Less [-]