While high-hydrostatic pressure (HHP) has successfully been applied to the pasteurization of fruit and vegetable juice beverages, their quality-stable shelf life during storage has not been fully elucidated. Therefore, we investigated the effect of HHP (550 MPa/10 min) treatment on polyphenols, carotenoids, ascorbic acids, and antioxidant capacity in tomato juice and their changes during 4-week refrigerated storage. High-temperature short-time (HTST, 110 °C/8.6 s) treatment was used as a control. The results revealed a significantly greater presence of polyphenols, carotenoids, ascorbic acid content, and antioxidant capacity in tomato juice after HHP processing than after HTST processing. However, the total carotenoids and total phenolic content in HHP-treated tomato juice decreased dramatically and approached that in the HTST-treated tomato juice after 1 week of storage. Therefore, HHP’s advantage in maintaining antioxidant compounds and capacity was only evident during the first week of storage in tomato juice. Nevertheless, the post-storage caffeic acid, quercetin, ferulic acid, and p-coumaric acid concentrations were 8.31, 4.77, 1.86, and 6.84 μg/g higher in the HHP-treated than in HTST-treated tomato juice, respectively. This study provides a new perspective for predicting HHP products' quality-stable shelf life.

Ferritin, as an iron storage protein, has been considered to be a well-utilized iron supplement. However, the typical thermal processing of food rich in ferritin may affect the structure and function of ferritin as an iron supplement. Here, a plant ferritin (soybean seed ferritin, SSF) and an animal ferritin (donkey spleen ferritin, DSF) were used to analyze the changes in fundamental structure and iron content after thermal treatments (68 °C for 30 min, 100 °C for 10 min). Then, SSF and DSF after thermal treatment were administered intragastrically to mice to further evaluate its digestive stability and absorptivity after thermal processing. Results showed the secondary structure, oligomeric states, iron content, and digestive stability of DSF were maintained better than that of SSF after thermal treatments, indicating that DSF has a higher thermostability than SSF. Both SSF and DSF after thermal treatment exhibited higher absorptivity than untreated ferritins. SSF showed higher absorptivity than DSF whether heated or not.

The potential of employing hyperspectral imaging (HSI) in the near-infrared (NIR) range (386.82−1,004.50 nm) for predicting the firmness of 'Fuji' apples cultivated in Aksu has been evaluated. The performance of seven preprocessing algorithms and two feature selection algorithms was evaluated. The coefficient of determination (R2) and root mean square error (RMSE) of Partial Least Squares (PLS) models are contrasted using various inputs. These results confirm that the Multiplicative Scatter Correction (MSC) preprocessing algorithm was the optimal choice (\begin{document}$ {R}_{p}^{2} $\end{document} = 0.7925, RMSEP = 0.6537), and the Competitive Adaptive Reweighted Sampling (CARS) feature selection algorithm demonstrated superior performance (\begin{document}$ {R}_{p}^{2} $\end{document} = 0.8325, RMSEP = 0.6257). Based on the aforementioned findings, PLS, Multiple Linear Regression (MLR), Heterogeneous Transfer Learning (HTL), and Back Propagation Neural Network (BPNN) models were constructed for cross-validation purposes. The experimental results indicate that the CARS-BPNN model exhibits the optimal prediction performance, with an \begin{document}$ {R}_{p}^{2} $\end{document} value of 0.9350 and an RMSEP value of 0.4654. The results of the research indicated that a deep learning method combined with hyperspectral imaging technology could be utilized to non-destructively detect the firmness of 'Fuji' apples, which will be beneficial and potentially applicable for post-harvest fruit firmness monitoring. This research provides a reference point for the non-destructive detection of apple in the selection of preprocessing, feature selection algorithms, and predicting firmness model.

In this paper, using a single-step method, resveratrol (RES)-loaded egg white protein (EWP) nanospheric particles were successfully prepared. The micelle behavior, micromorphology, molecular structure changes, and emulsifying properties of the nanoparticle were analyzed, and the molecular interaction between EWP and RES and the environmental response stability of the nanoparticle was characterized. The results show that I373/I385 dropped from 1.1 to about 0.8, indicating that high concentration of ethanol induced EWP to form a more hydrophobic and less polar structure. RES promoted the uniformity of the nanoparticle and formed a tightly-packed spherical three-dimensional structure by characterizing microstructure. Raman and infrared spectroscopy revealed enhanced hydrogen bonding between EWP and RES, increased g-g-g and t-g-t disulfide bonds, and the formation of three-dimensional helical structures due to the opening of flexible structural intervals. Molecular docking analysis identified hydrogen bonds and hydrophobic interactions as the main forces facilitating the binding between RES and EWP. Particle size analysis showed that D3,2 decreased from 30.51 to 17.88 μm, indicating better emulsion stability. The preservation of RES at 0.4 mg/mL was 94.49% in 50 mM NaCl and 83.68% in 500 mM NaCl, with no significant stability change (p > 0.05) over 48 h, revealing a concentration dependence of salt ions and storage stability of RES in the nanoparticle. This study establishes a foundation for exploring the incorporation of high-value hydrophobic compounds into EWP.

MicroRNAs (miRNAs) play important roles in various physiological activities in plants. However, their role in protecting grapes against gray mold (Botrytis cinerea) invasion remains largely unexplored. This study focuses on the phenotypic and physiological responses of 'Shine Muscat' (Vitis vinifera × V. labrusca) to gray mold infestation. High-throughput sequencing implicates several miRNAs, including miR398 and miR319, involved in the plant's defense mechanisms. Notably, miR395 emerges as a key player, positively influencing grape disease resistance. Specifically, miR395 downregulated the expression of its target gene APS1, which encodes ATP sulfurylase, a crucial enzyme in the plant's sulfur metabolic pathway. Concurrently, ATP sulfurylase downregulation increased the content of sulfate ions and glutathione (GSH). These findings were corroborated by our study of APS1. Collectively, these results suggest that miR395-APS1 modulates sulfur metabolism in grapes, thereby enhancing resistance to B. cinerea. The observed miRNA-mediated interactions between grapes and B. cinerea elucidate the role of miR395 in grape resistance to gray mold and offer new insights into the molecular mechanisms of grape disease resistance.

Machine learning, in combination with optical sensing, extracts key features from high-dimensional data for non-destructive food quality assessment. This approach overcomes the limitations of traditional destructive and labor-intensive methods, facilitating real-time decision-making for food quality profiling and robotic handling. This mini-review highlights various optical techniques integrated with machine learning for assessing food quality, including chemical profiling methods such as near-infrared, Raman, and hyperspectral imaging spectroscopy, as well as visual analysis such as RGB imaging. In addition, the review presents the application of robotics and computer vision techniques to assess food quality and then drives the automation of food harvesting, grading, and processing. Lastly, the review discusses current challenges and opportunities for future research.

Skeletal muscle atrophy resulting from glucolipid metabolic disorders poses a serious challenge to human health with the rapidly increasing prevalence of diabetes and obesity. Clinical trials investigating treatment interventions against skeletal muscle atrophy yielded limited success. This article addressed novel phytochemicals, such as polyphenols, flavonoids, terpenoids, alkaloids, and plant extracts, that modulated muscle atrophy and suggested avenues for future treatment. Several studies demonstrated an inverse relationship between dietary phytochemical supplementation and the onset of skeletal muscle atrophy caused by glucolipid metabolic disorders, as evidenced by improved muscle quality and function. Insulin-like growth factor 1/protein kinase B signaling pathway activation, protein ubiquitination inhibition, enhancement of mitochondrial function and inflammatory response, reduction of oxidative stress, and regulation of gut microbiota represent the mechanisms underlying the anti-skeletal muscle atrophy effect of phytochemicals. The manuscript also contains the clinical trials and filed patents regarding the beneficial effects of phytochemicals on skeletal muscle health. This review provided fresh perspectives on potentially effective therapeutic or preventive measures (dietary phytochemical intervention) for clinically managing skeletal muscle atrophy associated with diabetes or obesity.

Apple (Malus × domestica Borkh.) is a globally significant fruit in terms of both production and consumption. Metabolomics characteristics of 22 apple cultivars collected from five major apple-growing regions in Shanxi Province (China) were investigated by using 1H nuclear magnetic resonance (NMR) metabolomics. The analysis revealed significant variations in metabolite profiles among the cultivars, particularly in sugars (glucose, fructose, sucrose), asparagine, quinic acid, L-rhamnitol, phenylalanine, and condensed polyphenols. Notably, the cultivars 'Xinhongxing' and 'NY543' exhibited high levels of asparagine and quinic acid. 'Xinhongxing' had higher glucose levels but lower sucrose and fructose levels than other cultivars. 'Hongjiangjun' from higher altitudes showed elevated malate levels, indicating that environmental factors significantly influence malate metabolism in apple fruits. The study also revealed correlations between metabolites. For example, the content of condensed polyphenols was positively correlated with the level of asparagine, and that of quinic acid with phenylalanine. The study provides valuable insights on factors influencing apple composition and quality, underlining the importance of both genetic and environmental factors. Future research using transcriptomic and proteomic approaches could reveal the impact of gene-environment interaction on biochemical pathways involved in the primary and secondary metabolism of apples.

Apple (Malus × domestica Borkh.) is a globally significant fruit in terms of both production and consumption. Metabolomics characteristics of 22 apple cultivars collected from five major apple-growing regions in Shanxi Province (China) were investigated by using 1H nuclear magnetic resonance (NMR) metabolomics. The analysis revealed significant variations in metabolite profiles among the cultivars, particularly in sugars (glucose, fructose, sucrose), asparagine, quinic acid, L-rhamnitol, phenylalanine, and condensed polyphenols. Notably, the cultivars 'Xinhongxing' and 'NY543' exhibited high levels of asparagine and quinic acid. 'Xinhongxing' had higher glucose levels but lower sucrose and fructose levels than other cultivars. 'Hongjiangjun' from higher altitudes showed elevated malate levels, indicating that environmental factors significantly influence malate metabolism in apple fruits. The study also revealed correlations between metabolites. For example, the content of condensed polyphenols was positively correlated with the level of asparagine, and that of quinic acid with phenylalanine. The study provides valuable insights on factors influencing apple composition and quality, underlining the importance of both genetic and environmental factors. Future research using transcriptomic and proteomic approaches could reveal the impact of gene-environment interaction on biochemical pathways involved in the primary and secondary metabolism of apples.

In the process of post-harvest storage and transportation, the quality of fresh fruits and vegetables are decreased due to the autogenetic physiological effect and microbial pollution, which causes great losses to the food industry. Food packaging using edible film and coatings is an emerging environmentally friendly method of fruits and vegetable preservation. This review provides an overview of various film fabrication techniques, including solution casting, extrusion, electrospinning, and 3D printing, while examining the advantages and limitations of each method. A detailed analysis is offered on the key performance parameters of these films, such as mechanical strength, water vapor permeability, antioxidant activity, antimicrobial properties, and their effectiveness in preserving fruits and vegetables. Additionally, strategies to enhance the performance of edible films through incorporating nanoparticles, natural additives, and crosslinking methods are explored. The review aims to establish a comprehensive theoretical foundation and offer practical insights to support the further development and application of edible film technology in fruits and vegetables preservation.