The objective of the study was to establish the baseline values for blood and coagulation parameters in normal and healthy rusa deer (Rusa timorensis) of different ages and sexes. The samplepopulation consists of 40 rusa deer, divided into four groups of (i) juvenile males (ii) juvenile females (iii) adult males and (iv) adult females. The findings showed significant (p<0.05) higher values in erythrocyte count, calcium concentration and prothrombin time in the adult males compared to adult female rusa deer. On the other hand, the total protein concentration was significantly higher in adult females than adult male deer. No significant differences in blood or coagulation parameters were observed between sexes in the juvenile deer. Between age group, the adult deer had significantly higher mean cell volume, plasma protein and globulin concentration than juvenile rusa deer. Thus, it is necessary to take into account the age and sex of the rusa deer when using blood reference values for the diagnosis of diseases or health assessment.

A total of 23,322 specimens collected between 2014 and 2017, froma total of 2,592 cases, were received in Veterinary Research Institute, Ipoh (VRI) from various states in Malaysia and testedfor common bacterial, viral, and parasitic diseases in pigs. The highest occurrence of isolated bacteria from 771 samples whichtested positive were Salmonella (47.38%) and Escherichia coli (15.68%), followed by Staphylococcus (6.62%), Streptococcus (5.57%), Klebsiella pneumonia (4.88%), Pseudomona (3.38%), Acinetobacter (3.14%), Aeromonas (2.79%), Enterobacter (2.44%), one each of Bacillus and Pasteurella multocida (1.74%), Enterococcus (1.39%) and Corynebacterium (1.05%). 1.74% of each bacteria detected were Moxarella, Aspergillus, Burkholderia andChromobacterium. Positive samples tested by ELISA was Japanese encephalitis virus (JEV) (9.15%), Aujezsky disease virus (ADV)(5.37%), porcine cirvo-virus-2 (PCV2) (5.09%) and porcine reproductive and respiratory syndrome virus (PRRSV) (4.52%). Positive amples tested by the molecular test wasPCV2 (1.62%), PRRSV (1.32%) and classical swine fever virus (CSFV) (0.4%). Serology tests were conducted on 11,305 samplesand reported positive for Brucella suis (15.32%), Brucella abortus (0.62%), Brucella melitensis (0.85%), and melioidosis (0.05%). Parasitology analyses on 99 samples. revealed presence of 10.1% coccidia and 1% each of helminths and Sarcocystis. Within the 4-year period, there were no positive samples for porcine parvovirus (PPV), Nipah virus, swine influenza virus (SIV), and bacteria of Johne’s disease and leptospirosis. Continuous assessment is required to establish a comprehensive baseline data of swine diseases in Malaysia.

Multiple ovulation and embryo transfer is one of the reproductivetechnologies which is important to increase animal production. In this case report, four cows were selected as donors while elevencows were selected as recipients. Both donors and recipients had undergone the same procedures and steps for multiple ovulation and embryo transfer (MOET), such as cow preparation, synchronisation and where only donor cows follow the superovulation protocol. Cows were artificially inseminated and the embryos were flushed and graded. Out of four selected cows for donor, one of them was pregnant and only two cows actuallyproduced the embryos. The recovery rate for the embryo collection was 70.1%, and from 13 embryos (including unfertilised ova), 84.6% of the embryos was classified as good quality and suitable for embryo transfer. The overall results showed that out of eight recipients, five cows were detected pregnant, a 62.5% pregnancy rate. The aim of this report is to describe the procedures aswell as the factors that affect the successful of the MOET programme.

Biogas plant is an anaerobic digester that produces biogas fromorganic materials. The general purpose of implementing biogas plant in an animal farm is to obtain biogas from animal manure and the effluent of biogas digester after fermentation process can be used as organic fertiliser. There are 15 biogas plants based on livestock waste that have been developed throughout Malaysia until 2018. Two more biogas plants are planned to be built in Segamat and Keningau. All biogas plants built were originally aimed for domestic use which is as source of energyused for cooking and incinerator as well as to generate electricity for farm use. Out of 15 biogas plants, 7 plants were built on cattle farms, 4 plants on pig farms, 3 plantson poultry farms and only 1 plant on buffalo farm. Total construction cost for each biogas plant is between MYR4,500 to MYR30 million. Based on the survey conducted, it revealed that only 40% of the plants are still functional. However, 60% were dormant or nonfunctional due to poor maintenance, design errors, lack of technical knowledge to run the system consistently, improper planning and monitoring or insufficient livestock waste to continuously producing biogas.

Salmonella stained antigen has been widely used in Malaysia for detection of Salmonella infection in poultry. Growth phase of four Salmonella enterica serovar Pullorum (SP 9-25, SP 14/11, SP 690/79 and SP 7107/07) used in the antigen production were investigated based on colony enumeration and turbidity. This study aimed to determine the growth curve and the difference between S. Pullorum isolates based on turbidity measurement and spread plate technique for optimisation towards biomass production of salmonella antigen using bioreactor. Current production of the antigen used conventional methods and the number of bacterial cells is low and with several other drawbacks. The isolates were cultured in nutrient broth, incubated aerobically with constant shake for 48 hours to determine the lag, exponential, stationary and the death phase of the bacteria. Turbidity of the bacterial cells was measured using spectrophotometer and the colony was counted using total plate count every four hours. Based on the colony forming unit per milliliter, SP 690/79 strain showed the fastest growth where this bacteria achieved its mid-exponential growth at 8 hours. This is followed by SP 14/11 where this strain demonstrated the mid-exponential growth at 12 hours. The other two strains (SP 9-25 and SP 7107-07) are the slowest growth where their mid-exponential growth was measured at 14 hours. However, SP 690/79also the fastest strain entering the death phase which demonstrates the difference growth of the S. Pullorum strains. This study demonstrates that each S. Pullorum strains multiplying and dying at different phase though in the same serovar.

The livestock industry has been relying merely on chemically synthesised antibiotic for eye infections as sprays and ointment. A natural remedy from Curcuma spp. has been tested for efficacy in curing keratoconjunctivitis and uveitis. A severe case of uveitis was cured within 7 days, with impaired vision restored. These results were observations of a preliminary study conducted in a goat with uveitis.

Ginger, about one year old domestic shorthaired cat with paresis was reported with constipation. Enema and laxatives prior to presentation was not effective. On palpation of the abdomen, a long tubular mass was palpated in the left caudal abdomen. Radiographic diagnosis of the megacolon was made and a subtotal colectomy was planned for the cat. A ventral mid-line celiotomy was made to explore the abdomen. Exploration revealed ascending, transverse and descending colon including the caecum found to be markedly distended due to faecal stasis. Following standard technique, a subtotal colectomy was performed. The cat recovered well after surgery without any complication. The owner reported that Ginger was doing well but died about seven months after surgery. Carcass was not available for necropsy. By clinical examination, diagnostic investigation and surgical exploration, other causes of megacolon were ruled out. The cat was about one year old with a short duration of illness. Without a definite history and by ruling out other causes, neurologic trauma was attributed as the probable cause of constipation and megacolon in the present case.

This case report describes paramphistomosis from the rumen ofan infected 3-year-old Bos indicus or also known as Zebu cattle that was sent to the Veterinary Research Institute (VRI), Ipoh,Perak for post-mortem examination with a history of a sudden death. On post-mortem, it was found that the rumen contents hada large number of pink, pear shaped flukes, which measured about 1 cm in length attached to the rumen wall. On performing the sedimentation technique on the rumen contents, operculated eggs with germ cells were observed microscopically under compound microscope with a magnification of 100×. Regular screening of cattle for flukes is an important part of parasite controlprogrammes especially in endemic areas as it can cause anaemia and deterioration in body condition.

The local river terrapins (Batagur affinis) are an important aspect of the river ecosystem. The Turtle Conservation Centre in Bota Kanan noted morbidity and mortality in its terrapin population and some were sent for full laboratory investigationto diagnose cause of death. The faecal samples were subjected to faecal floatation technique whereby ascarid eggs were observed indicating that the turtles had helminth infection. Ascarid eggs had thick walls and measured at 140.6 μm to 149.7 μm in diameter. Apart from this, post-mortem examination of intestinal contents also showed presence of nematodes identified as Oesophagostomum sp. Treatment and control have to be instituted to prevent further morbidity and losses.

In the Malaysian environment horses are primarily used in sports activities such as racing, endurance, dressage and show jumping as well as in recreational pursuits and police work. Recently, the Perak Turf Club witnessed the death of a four-yearold thoroughbred mare which was given enrofloxacin injection as treatment and was regularly dewormed and vaccinated againstequine influenza, Japanese encephalitis and tetanus. Post-mortem examination of intestinal contents revealed presence of worms. The sample was then sent to the Veterinary Research Institute (VRI), Perak for morphological identification of the worm. The worm was identified as Parascaris equorum. Thus, awareness ongastrointestinal parasites should be raised especially by recommending improved management practices such as proper manure disposal and deworming procedures to control parasite infestations as well as good management and nutrition.