Twenty-three crossbred cows with endometritis were randomized into three groups. The animals of group I and II were treated with methanol fraction of neem oil and neem seed powder (25 ml each by intra-uterine route), respectively. Whereas, the control cows (gr III) were administered with groundnut oil at similar times. Efficacy of both neem preparations was assessed by Whiteside test (color reaction to cervico-vaginal mucus) and bacterial load at subsequent estrus. The results indicate that the administration of neem preparations retrieved the cows from endometritis as majority of them showed negative to Whiteside test (100 % in gr I and 62.5% in gr II) following treatment. Reduction in bacterial load was also of higher magnitude in neem-oil (96.02±2.02%) and seed-powder fraction (98.70±0.46%) treated animals compared to controls (24.97±29.64 %). Further, a higher pregnancy rate (71.42%) was obtained in oil fraction-treated cows than seed powder fraction-treated or control cows (25% each). In this study, the therapeutic efficacy of methanol fraction of neem oil appeared superior to neem seed powder in endometritic cows.

The study was conducted in 18 clinically healthy male dogs of about 1 year of age, weighing up to 12 kg and divided into three groups of 6 animals each. Bupivacaine alone, bupivacaine + hyaluronidase and hyperbaric bupivacaine were given epidurally at lumbosacral space @2 mg/kg. bwt. with a maximum dose of 3 ml in the animals of groups I, II and III, respectively. A significant increase (P0.01) in rectal temperature, heart rate and respiration rate was recorded at initial intervals of observation in all the groups and thereafter these values follow a declining trend in all groups consistently and reached to almost base line value at 120 min. of observation The alterations of glucose, serum urea nitrogen, aspartate aminotransferase and creatinine estimated at 1hr, 2hrs, 4hrs and 24 hrs were remained within physiological limits in all the groups.Therefore induction of regional anaesthesia with bupivacaine and its combination revealed no permanent alteration in the biochemical and physiological parameters hence it is safe and satisfactory anaesthesia.