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Studies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application: 1. Optimizing double antibody for progesterone in enzyme immunoassay
1988
Kang, C.B. | Shin, J.U. | Choe, S.Y. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Agruculture)
This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was 10X10** (3) to 15X10** (3), 20 and 1X10** (3) times, respectively
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