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Seroeactivity of Peruvian sheep and goats to small ruminant lentivirus-ovine progressive pneumonia virus.
1987
Madewell B.R. | Ameghino E. | Rivera H. | Inope L. | De Martini J.
Seroepizootiologic study of bovine respiratory syncytial virus in a dairy herd.
1986
Baker J.C. | Ames T.R. | Markham R.J.F.
Demonstration of vaccine-induced immunity to anaplasmosis without induction of persistent postvaccinal complement-fixing and agglutinating antibodies in yearling steers.
1985
Corrier D.E. | Johnson J.S. | Wagner G.C.
Characteristics and application of monoclonal antibody to progesterone, 2; Development of progesterone enzyme-linked immunosorbent assay (ELISA).
1991
Kang C.B. | Kim J.S.
Enzyme-linked immunosorbent assay for detection of antibody against hemorrhagic fever with renal syndrome virus in sera from experimentally infected rats
1987
Maeda, T. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Arikawa, J. | Takashima, I. | Hashimoto, N.
Evaluation of the potency, optimal antigen level and lasting immunity of inactivated avian influenza vaccine prepared from H5N1 virus
2009
Sasaki, T.(Kyoto Biken Lab. Inc., Uji (Japan)) | Isoda, N. | Soda, K. | Sakamoto, R. | Saijo, K. | Hagiwara, J. | Kokumai, N. | Ohgitani, T. | Imamura, T. | Sawata, A. | Lin, Z. | Sakoda, Y. | Kida, H.
Test vaccines comprised of inactivated water-in-oil emulsions containing various antigen levels were prepared using a non-pathogenic H5N1 avian influenza (AI) virus, A/duck/Hokkaido/Vac-1/04 (H5N1). The potencies of these test vaccines were evaluated by two experiments. In the first experiment, the triangular relationship among the antigen levels of test vaccines, the hemagglutination inhibition (HI) antibody response, and the protective effect against challenge with a highly pathogenic avian influenza (HPAI) virus, A/chicken/Yamaguchi/7/04 (H5N1), was confirmed. Then lasting immunity of chickens after a single-shot vaccination was confirmed in the second experiment. As a result, complete protection after the challenge was observed in chickens immunized by test vaccines with an antigen level of 160 HA units/dose or higher. Thus, it was ascertained that the minimum antigen level in the AI vaccine was 160 HA units/dose, and the minimum HI antibody titer that could protect chickens from HPAI virus infection-related death was considered to be 1:16. Dose-dependent HI antibody responses were observed in chickens after the vaccination. Thus, 640 HA units/dose were thought to be similar to the optimal antigen level. Alternatively, the HI antibody titers of chickens, injected with the vaccine containing 640 HA units/dose, were maintained at 1:181 or higher for 100 weeks after the single-shot vaccination.
Show more [+] Less [-]Development of enzyme linked immunosorbent assay for seromonitoring against hydropericardium syndrome (ELISA) virus
2003
Manzoor, R. | Kamal, T. | Khawaja, D.A. | Ismail, M.A. | Gill, Z.J. (Veterinary Research Inst., Lahore (Pakistan))
In this study ELISA was standardized for detecting antibodies against Hydropericardium Syndrome Virus. Factors like Antigen, Conjugate and incubation time were studied. Standardization was carried out by checker board titration method. Optimum incubation time for colour development was found to be 15 minutes while 50 mug/ml of antigen gave better results than 100 mug/ml. The conjugate dilution of 1:500 was observed to give satisfactory results as determined by regression analysis. Cut off value was determined by adding 2SDs and 3SDs in the mean optical density (OD) values of negative serum samples. It was found that mean OD+2SD (0.0684) resulted in better diagnostic sensitivity) and diagnostic specificity than mean OD+3SDs (0.0741).
Show more [+] Less [-]Production of hyperimmune serum against newcastle disease virus (NDV) in rabbits
2003
Iqbal, M. | Mahboob, K. | Zulfiqar, M. | Anwar-ul-Haque | Nabi, G. | Tabassum, R. (Veterinary Research Inst., Lahore (Pakistan))
The quick diagnosis of Newcastle disease requires known serum against the disease. In this study, an attempt was made to raise anti- Newcastle disease virus hyperimmune serum in rabbits. Three different inoculum were prepared to inoculate in the rabbits; (i) Fresh harvested allantoic fluid containing Newcastle disease virus (NDV) Mukteswar vaccine strain; (ii) Freshly harvested ND virus pelletted through centrifuging at 40,000 rpm for two hours and resuspended in normal saline and (iii) Pelletted virus (centrifuged and suspended as in ii) with addition of incomplete Freund's adjuvant. It was observed during the monitoring of haemagglutination inhibition (HI) titre that the serum collected after series of inoculation of 1st and 2nd inoculum provided maximum titre upto 1:256. However, the serum collected after series of injection of 3rd inoculum gave maximum HI titre 1:1024. This study suggested that antigen containing incomplete Freund's adjuvant provided better immune response against Newcastle disease virus in rabbits.
Show more [+] Less [-]Prevalence of bovine herpesvirus-1, parainfluenza-3, bovine rotavirus, bovine viral diarrhea, bovine adenovirus-7, bovine leukemia virus and bluetongue virus antibodies in cattle in Mexico
1983
Suzan, V.M. | Aguilar, R.E. (Direccion General de Sanidad Animal, Piso (Mexico)) | Onuma, M. | Murakami, Y.
Seroprevalance of Toxoplasma gondii infection in goats and sheep in Zimbabawe
2005
Hove, T. (Zimbabwe Univ., Harare (Zimbabwe). Dept. of Paraclinical Veterinary Studies) | Lind, P. | Mukaratirwa, S.