Refine search
Results 1-9 of 9
The nucleotide sequence of the genes, fanE and fanF, of Escherichia coli K99 fimbriae
1991
Ono, E. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Lavin, M.F. | Naiki, M.
Antigenic variation among equine H 2 N 8 influenza virus hemagglutinins
2001
Ozaki, H. (Hokkaido Univ., Sapporo (Japan)) | Shimizu Nei, A. | Sugita, S. | Sugiura, T. | Imagawa, H. | Kida, H.
A neutral sugar is responsible for serovar specificity of the antigenic determinant of Leptospira interrogans serovar Canicola
1994
Kashiwase, H. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Ono, E. | Yanagawa, R. | Shimizu, Y. | Kida, H.
Immunohistochemical studies on the localization of antigen-bearing phagocytes and lymphocytes in mouse lymph nodes after the germinal center formation
1984
Nakamura, K. | Hashimoto, Y. | Kitagawa, H. | Kudo, N. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
Evaluation of the potency, optimal antigen level and lasting immunity of inactivated avian influenza vaccine prepared from H5N1 virus
2009
Sasaki, T.(Kyoto Biken Lab. Inc., Uji (Japan)) | Isoda, N. | Soda, K. | Sakamoto, R. | Saijo, K. | Hagiwara, J. | Kokumai, N. | Ohgitani, T. | Imamura, T. | Sawata, A. | Lin, Z. | Sakoda, Y. | Kida, H.
Test vaccines comprised of inactivated water-in-oil emulsions containing various antigen levels were prepared using a non-pathogenic H5N1 avian influenza (AI) virus, A/duck/Hokkaido/Vac-1/04 (H5N1). The potencies of these test vaccines were evaluated by two experiments. In the first experiment, the triangular relationship among the antigen levels of test vaccines, the hemagglutination inhibition (HI) antibody response, and the protective effect against challenge with a highly pathogenic avian influenza (HPAI) virus, A/chicken/Yamaguchi/7/04 (H5N1), was confirmed. Then lasting immunity of chickens after a single-shot vaccination was confirmed in the second experiment. As a result, complete protection after the challenge was observed in chickens immunized by test vaccines with an antigen level of 160 HA units/dose or higher. Thus, it was ascertained that the minimum antigen level in the AI vaccine was 160 HA units/dose, and the minimum HI antibody titer that could protect chickens from HPAI virus infection-related death was considered to be 1:16. Dose-dependent HI antibody responses were observed in chickens after the vaccination. Thus, 640 HA units/dose were thought to be similar to the optimal antigen level. Alternatively, the HI antibody titers of chickens, injected with the vaccine containing 640 HA units/dose, were maintained at 1:181 or higher for 100 weeks after the single-shot vaccination.
Show more [+] Less [-]Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain
2008
Daud, N.H.A.(Hokkaido Univ., Sapporo (Japan)) | Kariwa, H. | Tkachenko, E. | Dzagurnova. T. | Medvedkina, O. | Tkachenko, P. | Ishizuka, M. | Seto, T. | Miyashita, D. | Sanada, T. | Nakauchi, M. | Yoshii, K. | Maeda, A. | Yoshimatsu, K. | Arikawa, J. | Takashima, I.
Puumala virus (PUUV), a causative agent of hemorrhagic fever with renal syndrome (HFRS), is prevalent in Europe and European Russia. No vaccine has been developed for PUUV-associated HFRS, primarily because of the low viral yield in cultured cells. A PUUV strain known as DTK/Ufa-97 was isolated in Russia and adapted for growth in Vero E6 cells maintained in serum-free medium. The DTK/Ufa-97 strain produced a higher viral titer in serum-free medium, suggesting that it may prove useful in the development of an HFRS vaccine. When PUUV-infected Vero E6 cells were grown in serum-free medium, the DTK/Ufa-97 strain yielded more copies of intracellular viral RNA and a higher viral titer in the culture fluid than did the Sotkamo strain. Phylogenetic analysis revealed that PUUVs can be classified into multiple lineages according to geographical origin, and that the DTK/Ufa-97 strain is a member of the Bashkiria-Saratov lineage. The deduced amino acid sequences of the small, medium, and large segments of the DTK/Ufa-97 strain were 99.2% to 100%, 99.3% to 99.8%, and 99.8% identical, respectively, to those of the Bashkirian PUUV strains and 96.9%, 92.6%, and 97.4% identical, respectively, to those of the Sotkamo strain, indicating that the PUUVs are genetically diverse. However, DTK/Ufa-97 and other strains of PUUV exhibited similar patterns of binding to a panel of monoclonal antibodies against Hantaan virus. In addition, diluted antisera (i.e., ranging from 1:160 to 1:640) specific to three strains of PUUV neutralized both homologous and heterologous viruses. These results suggest that the DTK/Ufa-97 strain is capable of extensive growth and is antigenically similar to genetically distant strains of PUUV.
Show more [+] Less [-]A survey of canine echinococcosis in Gobi Altai Province of Mongolia by coproantigen detection
2001
Zoljargal, P. (Hokkaido Univ., Sapporo (Japan)) | Ganzorig, S. | Nonaka, N. | Oku, Y. | Kamiya, M.
Few studies have been carried out for the prevalence of canine echinococcosis in Mongolia. This study was designed to elucidate a preliminary information of the prevalence from feces collected in the field. Sixty-seven fecal samples from dogs and 2 red foxes in Altai town were collected and examined for Echinococcus coproantigen and eggs. Coproantigen detection was performed by a sandwich ELISA using a monoclonal antibody EmA9 raised against Echinococcus multilocularis somatic antigen. Of the dog samples examined, 17 (25.4%) were positive by the ELISA. One out of two foxes was positive, too. Taeniid egg-positive feces were recognized in 12 dog feces. Only 6 samples were both coproantigen and egg positive. Eggs of Ancylostoma sp., Trichuris sp., and Capillaria sp.; were also registered.
Show more [+] Less [-]Evaluation of coproantigen diagnosis for natural Echinococcus multilocularis infection in red foxes [Vulpes vulpes]
1999
Morishima, Y. (Hokkaido Univ., Sapporo (Japan)) | Tsukada, H. | Nonaka, N. | Oku, Y. | Kamiya, M.
The validity of a coproantigen ELISA for Echinococcus multilocularis was evaluated by comparison of three diagnostic methods; autopsy, egg examination and the ELISA. Of 71 foxes, 39 were found to be infected with the cestode at autopsy. The overall mean of worm burdens was 3,451, but the number varied (1-34,522). The ELISA could detect 94.9% (37/39) of the worm positives and there were no false-positives. Two false-negatives were infected with 1 and 4 cestodes, whereas 3 cases with similar worm burdens (2, 4 and 6 worms) were diagnosed as positives. This indicates the detection limit of the assay may be equivalent to less than 10 (in the worm burden). On the other hand, egg examination showed low sensitivity (43.6%, 17/39). These results suggest the ELISA has a potential to replace for the conventional methods
Show more [+] Less [-]Use of biotinylated antibody for the assay of Hanganutziu-Deicher antibodies and antigens in fluids and tissues from cancer patients
1989
Gathuru, J.K. (Yokohama Univ. (Japan). Faculty of Engineering) | Higashi, H. | Kato, S. | Usuba, O. | Naiki, M.