In the conditions of the Republic of Belarus there was studied the interrelation between content of major nutrient elements (calcium and phosphorous) and trace elements (iron, copper, cobalt, and manganese) in forages and blood of first-calf cows at the first and last stages of lactation. Also, there were developed formulas of mixed forages and a premix for these animals. The studies were realized at the Agricultural Production Co-operative Olgovskoe of Vitebsk region (Belarus). Research of analysis of forages and state of mineral metabolismat heifers made it possible to draw following conclusions: in cow rations there was marked the deficiency of vitamin D, as well as some trace elements (cobalt and manganese) in the conditions of calcium and phosphorus excess. In spite of a high content in a calcium in diets, it was noted than in blood plasma the concentration of this element was below the standard for the majority of animals. Lack of cobalt and manganese of whole blood of cows was connected with a low level of these elements in cow diets. The surplus contents of copper in forages and blood of animals against the background low activity of ceruloplasmin the weakened antioxidant defense of heifer cows. For preventive treatment of possible hypo trace element deficiencies and metabolic disorders of heifer cows at the final stage of lactation there can be recommended the correcting of cow diets with inclusion into their composition of the developed for compound of mixed fodder and premix.

In the conditions of the Republic of Belarus there was analyzed the influence of inclusion into broiler chickens diets of probiotoc preparation Bifidoflorin zhidkij (liquid) and prediotic veterinary drug Biofon AIL on the natural resistance, hematopoiesis, growth, metabolic function, development and viability of broiler chickens. In course of the study there was analyzed the dynamics of total haemoglobin, total protein of blood serum, albumins and globulins of broiler chickens which were fed with veterinary drugs probiotoc Bifidoflorin zhidkij and prediotic Biofon AIL. Indexes of chicken viability and life weight gain were marked. Research results showed that application of the presented veterinary preparations rendered the growth promoting action, lowering of disease incidence, increasing of chicken viability, increasing of live weight gain and stimulation of metabolic processes in the most intense period of poultry growing. All these factors were expressed in highest degree after the combined application of the probiotic and prebiotic veterinary drugs.

In the conditions of the Republic of Belarus there was studied the possibility of increasing of meat yield productivity of ducklings of Temp cross in the conditions application of parental lines of Peking ducks of German breeding Stolle Seddin Vital (SVV) cross. In course of the study there was realized a comparative evaluation of linear and crossbred ducklings in accordance with meat qualities and blood indexes. There were presented the results of morphological composition of liner and crossbred duck carcasses; dynamics of changes of lipid exchange (total lipids; cholesterine; triglyceride) of ducklings; morphological composition of carcasses of father and mother combinations; blood indexes of ducklings; blood indexes of crossbred ducklings of final combinations. Research results showed that crossbred ducklings had high meat properties which exceeded the purebred ones. In ducklings with lower indexes of fattiness of carcasses there was noted the lowering of concentration of total lipids, cholesterine and triglyceride in blood.

Results of studies of peculiar properties of progesterone content in blood serum of outbred female rats under the influence of intensive ionizing radiation against the background of stress stimulation realized in the conditions of the Republic of Belarus were presented. There were the following animal groups: 1. controlled rats; 2. irradiated rats; 3. stressed animals; 4. rats subjected to ionizing radiation and stress. Research results showed that concentration of progesterone in serum blood of female rats in 3 days after immobilized stress decreased on 40% in comparison with the control indexes. More intensive decreasing of hormone level took place after irradiation in dose of 1 Gy (on 62% in comparison with the controlled group). Combine activity of ionizing radiation and stress involved the decreasing of hormone concentration on 25% in comparison with the controlled group. In ten days there was the restoration of changes. Increase of progesterone concentration in blood serum took place in all tested groups. Hormone level in blood serum of irradiated rats increased on 46,04 nmol/l in comparison with results of the third day after irradiation. Hormone level in blood serum of stressed animals increased on 11,63 nmol/l, in group subjected to the combined activity of ionizing radiation and stress – on7,65nmol/l. On the thirtieth day the indexes of progesterone concentration in blood serum of irradiated rats, stressed rats and rats subjected to ionizing radiation and stress were almost similar to the control indexes

Determination of degree and relations between derangements of metabolic processes in calves with respiratory syndrome in the milking period was realized in the conditions of the Republic of Belarus. Research results showed that the calves with the respiratory syndrome had expressed metabolic processes disturbances, which were marked in the analyzed biochemical blood indexes (total protein; albumins; urea; creatinine; glucose; cholesterin; triglycerids; bilirubin; ALP; AAT; SGPT; calcium; phosphorous; magnesium; iron; manganese; cobalt; cuprum; zinc). In calves with respiratory syndrome there was noted the increased content of crude protein in blood serum (71,42 +/- 2,57 g/l P less than 0,05), low urea level (3,08 +/- mmol/l). There was the increased level of creatine (574,11 +/- 65,26 mkmol/l; P less than 0,01) and bilirubin (8,11 +/- 1,15 mkmol/l; P less than 0,05). Glucose concentration in blood of diseased calves was at below standard level (3,49 +/- 0,69 mmol/l). Glucose indexes varied and were changing during a day; and depended on muscular activity, intervals between feeding and hormonal regulation. Diseased calves had high cholesterol level in blood serum (4,07 +/- 0,34 mmol/l; P less than 0,05), which was stated in case of liver disease and hypothyroidism. Phosphor level was higher than the standard indexes of healthy calves (2,85 +/- 0,03 and 2,05 +/- 0,05, respectively). Content of magnesium, cobalt and zinc in blood of the diseased calves was lower than in healthy ones. Calves with respiratory pathology had protein, fat, hydro-carbonic and mineral metabolic processes

Determination of possible relations between some metabolic activity indexes of Large White pregnant sows (with various pregnancy stages) and their offspring was realized in the conditions of the Republic of Belarus. In the experimental animals there was taken blood for hematologic and biochemical tests. In peripheral blood there was analyzed the average content of hemoglobin, total number of erythrocytes, leukocytes, thrombocytes and corpuscular volume. Content of total protein, albuminolesterin, concentration total kalium and nonorganic phosphorus, magnesium, iron, zinc, activity of alanine- and aspartate transaminase were evaluated in blood serum. Blood tests were made on 84th day of pregnancy, in 30 days before farrowing, on the 2-nd lactation day and on the 2-nd and 35-th days of piglet life. Research results showed high degree of persistence of iron and zinc deficiency of pregnant sows in the conditions of modern national swine production. The obtained results proved the presence of close relationship between metabolic processes of sows and piglets that was revealed in low degree of stores supply with elements which had been in deficit for their mothers

Evaluation of safety and efficiency of application of immunostimulative drug Izambenum (C14H15IN2O) for stimulation of natural resistance (autarcesis) and immunologic responsiveness of chickens of laying breeds was realized in the conditions of the Republic of Belarus. Clinical and experimental investigation of Izambenum influence on poultry for the increasing of their resistance, activation of immunological response to vaccination against Newcastle disease, stimulation of their growth and development was realized. Research results showed that Izambenum influenced on level of morphological indexes of chicken blood: number of erythrocytes increased on 24%, hemoglobin - on 17%. Total protein in blood serum increased in all test period and number of lymphocytes increased and differential was 90-100%. Izambenum increased strains of post vaccination immunity to Newcastle disease. Bacterial growth-inhibitory activity decreased on the 27the day of life and increased at the same positions on the 34-41 days. Phagocyte activity of pseudoeosinophils increased in all test groups. Research results showed that Izambenum rendered growth and immunity stimulation action and increased their viability. Its immunostimulative action was conditioned by strengthening of cellular and to lower extent humoral immunity factors. Application of the preparation in period of Newcastle disease vaccination promoted the formation of long-lived immunity

Determination of a degree and stages of metabolic disorders of aborted sows and sows which gave birth to dead fetus was realized in the conditions of the Republic of Belarus. Analysis of biochemical profile of blood of experimental sows was given. Typical deviations of biochemical status indexes in the conditions of reproduction pathology were determined. The highest degree of deviations of biochemical blood profile (indexes of fat, protein and mineral metabolism) was stated at aborted sows. At sows which gave birth to dead fetus there were stated abnormalties functional state of kidneys and liver, indexes of carbohydrate and mineral metabolism.

Research on working out of a new fodder additive on the basis of rape (Brassica napus) phospholipids which included mineral substances necessary for normal functioning of cattle was realized in the conditions of the Republic of Belarus. Results of biochemical analysis of 35240 blood samples revealed that 15-40% of studied animals had abnormalties in vitamin, protein and mineral metabolism and lowering of immunobiological disease resistance. There was correlation dependence between the degree of metabolic orders and the state of forage reserve of a forage reserve. The lowering of forage reserve caused the increasing of metabolic disorders. As a whole forages of the surveyed farms were low in content of copper, zinc, cobalt and selenium and could not provide physiological requirements of animals without introduction into rations of specialized additives

In the conditions of immunization of swine by experimental produced at Vitebsk Biofactory in the Republic of Belarus vaccines in blood of experimental swine there stated the morphological changes which pointed to formation of immune response.