In the conditions of the Republic of Belarus there were studied biochemical indexes of calf blood serum against the background of application of probiotic preparations Bilavet C. Results of research showed that administration of probiotic preparations from the first day till the six day and from the fourteenth day till the nineteenth day of calf life with the preventive purpose rendered the simulative influence on indexes of natural resistance, which were accompanied with the increasing of total protein quantity in blood on 2,9-5,9%, as well as the increasing of protein fractions, in particular, globulin-rich fraction on 4,2-6,4%. Administration of probiotic preparation Bilavet С to calves rendered the positive influence on blood biochemical indexes, promoted the uptaking and better accumulation of mineral substances: content of crude calcium, inorganic phosphorus, magnesium, iron increased on 15,3%, 10,5%, 13,2% and 3,6%, respectively.

In the conditions of the Republic of Belarus there was analyzed the efficiency of administration of nonconventional feed materials in diets of highly productive cows and its influence of indexes of natural resistance at different physiological stages of animals. In the process of studying of adaptation processes of highly productive cows at different stages of lactation it was determined that feeding with nonconventional melanoidin-humic additives at different physiological periods rendered a positive influence of highly productive cows, promoted the increasing of natural resistance of animals and improvement of immuno-humoral blood indexes. Administration of an additive Ehkolin in a ration of highly productive cows with stimulation for increasing milk flow in a dose of 100 ml per head promoted the increasing of blood serum bactericidal activity on 5,2%, beta-lysine activity - on 7,2%. Feeding of animals at a basic lactation period with 50 ml of preparation Ehkolin-2 provided the maximum increasing of lysozyme activity of blood serum (on 6,2%) and beta-lysine activity of blood serum (on 12,7%), and also promoted the activation of leukocytopoiesis. Addition of Ehkolin-3 in ration structure of dry cows in a dose of 750 ml increased the indexes of lysozyme activity of blood serum on 7-8,6%, bactericidal activity of blood serum - on 2,2-3,6% and beta-lysine activity of blood serum - on 2,2-3,6%.

Results of studies of peculiar properties of progesterone content in blood serum of outbred female rats under the influence of intensive ionizing radiation against the background of stress stimulation realized in the conditions of the Republic of Belarus were presented. There were the following animal groups: 1. controlled rats; 2. irradiated rats; 3. stressed animals; 4. rats subjected to ionizing radiation and stress. Research results showed that concentration of progesterone in serum blood of female rats in 3 days after immobilized stress decreased on 40% in comparison with the control indexes. More intensive decreasing of hormone level took place after irradiation in dose of 1 Gy (on 62% in comparison with the controlled group). Combine activity of ionizing radiation and stress involved the decreasing of hormone concentration on 25% in comparison with the controlled group. In ten days there was the restoration of changes. Increase of progesterone concentration in blood serum took place in all tested groups. Hormone level in blood serum of irradiated rats increased on 46,04 nmol/l in comparison with results of the third day after irradiation. Hormone level in blood serum of stressed animals increased on 11,63 nmol/l, in group subjected to the combined activity of ionizing radiation and stress – on7,65nmol/l. On the thirtieth day the indexes of progesterone concentration in blood serum of irradiated rats, stressed rats and rats subjected to ionizing radiation and stress were almost similar to the control indexes

Determination of degree and relations between derangements of metabolic processes in calves with respiratory syndrome in the milking period was realized in the conditions of the Republic of Belarus. Research results showed that the calves with the respiratory syndrome had expressed metabolic processes disturbances, which were marked in the analyzed biochemical blood indexes (total protein; albumins; urea; creatinine; glucose; cholesterin; triglycerids; bilirubin; ALP; AAT; SGPT; calcium; phosphorous; magnesium; iron; manganese; cobalt; cuprum; zinc). In calves with respiratory syndrome there was noted the increased content of crude protein in blood serum (71,42 +/- 2,57 g/l P less than 0,05), low urea level (3,08 +/- mmol/l). There was the increased level of creatine (574,11 +/- 65,26 mkmol/l; P less than 0,01) and bilirubin (8,11 +/- 1,15 mkmol/l; P less than 0,05). Glucose concentration in blood of diseased calves was at below standard level (3,49 +/- 0,69 mmol/l). Glucose indexes varied and were changing during a day; and depended on muscular activity, intervals between feeding and hormonal regulation. Diseased calves had high cholesterol level in blood serum (4,07 +/- 0,34 mmol/l; P less than 0,05), which was stated in case of liver disease and hypothyroidism. Phosphor level was higher than the standard indexes of healthy calves (2,85 +/- 0,03 and 2,05 +/- 0,05, respectively). Content of magnesium, cobalt and zinc in blood of the diseased calves was lower than in healthy ones. Calves with respiratory pathology had protein, fat, hydro-carbonic and mineral metabolic processes

Evaluation of safety and efficiency of application of immunostimulative drug Izambenum (C14H15IN2O) for stimulation of natural resistance (autarcesis) and immunologic responsiveness of chickens of laying breeds was realized in the conditions of the Republic of Belarus. Clinical and experimental investigation of Izambenum influence on poultry for the increasing of their resistance, activation of immunological response to vaccination against Newcastle disease, stimulation of their growth and development was realized. Research results showed that Izambenum influenced on level of morphological indexes of chicken blood: number of erythrocytes increased on 24%, hemoglobin - on 17%. Total protein in blood serum increased in all test period and number of lymphocytes increased and differential was 90-100%. Izambenum increased strains of post vaccination immunity to Newcastle disease. Bacterial growth-inhibitory activity decreased on the 27the day of life and increased at the same positions on the 34-41 days. Phagocyte activity of pseudoeosinophils increased in all test groups. Research results showed that Izambenum rendered growth and immunity stimulation action and increased their viability. Its immunostimulative action was conditioned by strengthening of cellular and to lower extent humoral immunity factors. Application of the preparation in period of Newcastle disease vaccination promoted the formation of long-lived immunity

Evaluation of antagonistic activity of strains of lactic and bifid bacteria, metabolite producers, as well as the treatment and prophylactic efficacy of Laktimet and Batsinil probiotics was realized in the conditions of the Republic of Belarus. In course of study there were estimated 29 strains of lactic acid bacillus of different kinds, 13 strains of bifid bacteria and 35 strains of bacillus (Bac. subtilis and Bac. pumilis). There were studied 3 strains of bifid bacteria: Bifidobacterium adolescentis MS-42; Bifidobacterium adolescentis BIM V-01; Bifidobacterium adolescentis BIM V-87; 2 strains of lactic acid bacillus: Lactococcus lactis subsp. Lactis BIM V-132, Lactococcus casei subsp. rhamnosus BIM V-189; and 6 strains of Bacillus subtilis. Research results showed that the main part of the studied strains had steady antagonistic activity to test-cultures of opportunistic pathogenic bacteria. Zone of growth delay of test-cultures of the main quantity of strains (from 54 up to 92%) was from 11 up to 30. Research results showed that application of cell-free probiotics Laktimet and Batsinil promoted the increasing of resistance by means of increasing or lowering of total protein, insignificant increasing of blood serum bactericidal activity and increasing of phagocytic activity of leukocytes on 7,3%

Serological properties of leptospirae bacteria grown on differential nutritive media were studied by the example of sheep in the conditions of the Republic of Belarus. In the experiment there were used 6 pathogenic industrial leptospirae stock: Leptospira pomona VGNKI-6; Leptospira tarassovi VGNKI-4; Leptospira grippothyphosa VGNKI-1; Leptospira icterrohaemorragia VGNKI-2; Leptospira conicola VGNKI-3; Leptospira hardjo VGNKI-5. The results showed a serological difference of leptospirae grown on conventional and experimental media derived from ram's blood.

Investigation of influence of water improved by potassium permanganate solution on welfare and productivity of piglets was realized in the conditions of swine breeding complex in the Republic of Belarus. In the experiment there were used 2 groups of 30-day old piglets. In course of 75 days of experiment the running water of pig complex was used for watering of piglets of control group. Piglets from the experimental group were given water with addition of 0,1% solution of potassium permanganate. Analysis of obtained results showed that water with potassium permanganate promoted the increasing of natural resistance of piglets. At the same time there was stated the increasing of lysozyme and bacterial activityin blood serum. Application of improved water promoted the increasing hemoglobin on 4,8-4,5%, erythrocytes - on 2,9-4,2% in comparison with control. Content of total protein increased on 2,2-2,8% in the experimental groups, lisozyme activity - 6,9-7,0% and bacterial activity - on 5-6,9%. Application of improved water did not influence the glucose and potassium concentration in blood. In the experimental group there was stated the increased lipid concentration on 3,1-6%, cholesterol - on 4,4-6,3%, potassium level - on 2,5-4,5%, phosphorus - on 0,7-5,3%, sodium - on 1,4-3,8%. Application of water with КMnО4 increased average daily weight gain on 5,2-4,2%

Influence of monocalcium phosphate on natural resistance and clinical indexes of blood of breeding bull calves of white-and-black breed was realized in the conditions of the Republic of Belarus. Bull calves of the first (control) group in the composition of their main ration were fed with mixed fodder K-66-b, hay, green mass and as a balanced phosphoric-potassium additive they were fed with8 grams of defluorinated phosphate per 1 kg of mixed feed. Bulls of the second, third and fourth experimental groups in combination with the standard ration instead of difluoridated phosphate were fed with 5; 10; and 15 grams monocalcium phosphate per 1 kg of combined feed, respectively. Research results showed that application of fodder monocalcium phosphate in addition to the main ration made it possible to increase calcium and phosphorus content in blood of experimental bull calves. In the control group phosphorous content was 2,17 micromole, in the experimental groups – 2,40-2,78 micromole; calcium content – 2,50 and 2,58-2,88 micromole, respectively. The analyzed additive rendered positive effect on protein exchange, increased the natural resistance, bacterial growth-inhibitory activity in blood serum in the third, fourth and second groups on 50,3%, 42,5%, and 40,3%, respectively; lysozyme activity – on 46,0%, 39,2%, and 32,3%, respectively; phagocytic activity of leukocytes – on 21,9%, 25%, and 19,1%

Investigation of immunity of broiler chickens of Kobb-500 cross vaccinated by national associated viral vaccine and foreign (ABIC, Israel; Vitex XXI vek Seva) vaccines against Newcastle disease and infectious bronchitis was investigated in the conditions of the Republic of Belarus. In course of studies there was analyzed the content of immunoglobulin in blood serum, as well as the influence of living vaccines on immunity formation. Research results showed that broiler chickens immunization against Newcastle disease and infectious bronchitis by national and foreign vaccine produced by ABIC company made it possible to increase the phagocytic activity of pseudoeosinophils, bactericidalal and lysozyme activity of blood serum, that proved the activation of nonspecific protection factors. At the same time in blood serum there was stated the increasing of immunoglobulin number in 1,5-3,0 times in comparison with control groups. Economic efficiency of all analyzed vaccines was analyzed