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Baculovirus expression and potential diagnostic application of the gp51 envelope glycoprotein of genetic mutants of the bovine leukaemia virus
2019
Rola-Łuszczak Marzena | Grabowska Agnieszka | Szewczyk Bogusław | Kuźmak Jacek
Introduction: Field isolates of bovine leukaemia virus (BLV) show the presence of a few amino acid substitutions in major conformational G and H epitopes on surface glycoprotein gp51. Potentially, these substitutions can affect the 3D structure of these epitopes leading to their diminished immunoreactivity. The aim of this study was to express three gp51 glycoproteins carrying mutated epitopes as recombinant baculovirus proteins in insect cells to test their immunoreactivity with bovine sera.
Show more [+] Less [-]Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis
2022
Szczotka Maria | Kuźmak Jacek
Bovine leukaemia virus (BLV) is the retroviral causative agent of enzootic bovine leukosis, the most common neoplastic disease of cattle and a serious problem worldwide. Its diagnosis is commonly by tests for antibodies recognising the p24 capsid protein and structural glycoprotein (gp) 51. With flow cytometry recently having come to veterinary immunology, applications for it may now include BLV. The study determined BLV gp51 expression in blood and milk lymphocytes of naturally infected cows by flow cytometry.
Show more [+] Less [-]A clinical case of enzootic bovine leukosis diagnosed by malignant lymphoma cells detected in urinary sediment
2019
Sakaguchi, K. (Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido (Japan). Department of Veterinary Medicine) | Ougitani, M. | Maezawa, M. | Tanaka, Y. | Watanabe, K. | Horiuchi, N. | Kobayashi, Y. | Inokuma, H.
Vector transmission of bovine leukemia virus during summer season in northern Hokkaido
2019
Inagaki, H. (Souya Livestock Hygiene Service Center, Hamatonbetsu (Japan)) | Konnai, S. | Kaburagi, H. | Murota, H. | Takabatake, N. | Watari, K. | Okagawa, T. | Maekawa, N. | Murata, S. | Ohashi, K.
Experimental transmission of bovine leukemia virus in cattle via rectal palpation
2006
Kohara, J.(Hokkaido. Animal Research Center, Shintoku (Japan)) | Konnai, S. | Onuma, M.
We examined whether Bovine leukemia virus (BLV) was transmitted by rectal palpation using a common sleeve between a BLV-infected cow and BLV- negative steers. Three of four steers developed antibodies against BLN as determined by agar-gel immunodiffusion (AGID) test between 7 to 10 weeks after the first rectal palpation using common sleeves from BLV-infected cow. In the steers, BLV proviral DNA were detected by PCR 1 to 5 weeks earlier than detection of the antibodies by the AGID test. Our experiments demonstrated that rectal palpation is a potential cause of BLV spread in herds and that detection of BLV proviral DNA in cattle by PCR is useful screening test for early diagnosis of BLV infection.
Show more [+] Less [-]Cattle with a low bovine leukemia virus proviral load are rarely an infectious source
2018
Mekata, H. ((University of Miyazaki, Miyazaki (Japan). Organization for Promotion of Tenure Track), (University of Miyazaki, Miyazaki (Japan). Center for Animal Disease Control)) | Yamamoto, M. | Hayashi, T. | Kirino, Y. | Sekiguchi, S. | Konnai, S. | Horii, Y. | Norimine, J.
Evidence of bovine immunodeficiency virus in cattle in Turkey
2003
Meas, S. (Hokkaido Univ., Sapporo (Japan)) | Yilmaz, Z. | Usui, T. | Torun, S. | Yesilbag, K. | Ohashi, K. | Onuma, M.
Bovine leukemia virus genotype 1 and 6 are circulating among dairy and beef cattle of small and medium holding farms in northern Vietnam
2019
Dao, T.D. ((Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido (Japan). Department of Veterinary Medicine), (National Institute of Veterinary Research, Hanoi (Vietnam)) | Nguyen, H.T. | Than, S.T. | Bui, V.N. | Ogawa, H. | Imai, K.
A clinical case of enzootic bovine leukosis in a 13-month-old Holstein heifer
2018
Maezawa, M. ((Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido (Japan). Department of Veterinary Medicine), (Gifu University, Gifu (Japan). United Graduate School of Veterinary Sciences)) | Watanabe, K. | Horiuchi, N. | Matsumoto, K. | Kobayashi, Y. | Inokuma, H.
Evidence for bovine immunodeficiency virus infection in cattle in Zambia
2004
Meas, S. (Hokkaido Univ., Sapporo (Japan)) | Nakayama, M. | Usui, T. | Nakazato, Y. | Yasuda, J. | Ohashi, K. | Onuma, M.
