A total of 281 serum samples collected randomly from goats showing the signs of fever, abortion,repeat breeding and still births as well as from apparently healthy ones were subjected to LAT and MAT based on rLipL32 and rLipL41 antigens. A total of 16 (5.69%) samples were found positive to MAT, whereas rLipL32-LAT and rLipL41-LAT detected 35 (12.45%) and 23 (8.18%) samples as positive, respectively. The sensitivity and specificity of rLipL32-LAT was 87.50% and 92.83%, respectively,while rLipL41-LAT yielded 75.00% and 97.35% sensitivity and specificity, respectively. LAT based on rLipL32 and rLipL41antigens could further be evaluated on a larger number of samples to ensure its utility as a screening test for the sero-epidemiological studies.

A study was conducted on the epididymis of 30 Gaddi goats, divided into three groups of 10 animals in each viz; Prepubertal (1 day old to 18 months of age), Pubertal (18 months to 5yrs of age)and Postpubertal (5yrs of age). The study revealed that all the structures of epididymis grew gradually and consistently with age; however the differential growth was more appreciable during prepubertal and pubertal stages of life than in the later phases. In one day old kids, the tubular epithelium comprised of simple columnar epithelium, which became pseudostratified columnar at six months of age. The tubular diameter and height of the epithelium and its stereocilia also increased from birth to late prepuberty. It increased further in the pubertal animals and only slightly in postpubertal animals. Histochemically the cytoplasm of epididymis was PAS positive and Sudanophilic. Moderate to strong ACPase and strong AKPase activities were observed in all the segments of epididymis, which increased with age.