Studying of efficiency of application of modified scheme of hyperimmunization of bull-producers with a designer salmonella polyantigenin in comparative analysis with the production scheme of hyperimmunization was realized in the conditions of the Republic of Belarus. Research results showed that engineered polyvalent antigen for hyperimmunization of producer bulls contained in their formula the following serovariants of salmonella in 1:1 ratio: S. typhimurium 371, S. dublin 373, S. choleraesuis 370, and S. enteritidis CB. The obtained antigen was sterile, harmless and had pH 7,3. Hyperimmunization of producer bulls in the experimental scheme revealed in increasing of leukocytes quantity on 57,3 %, banded neutrophils on 86,7 %, B-lymphocytes - on 42,2 %, content of total protein - on 25,6 %, content of Ig G - on 77,8 %, lg M - on 51,6%. After the fifth administration of antigen there was stated the lowering of agglutinating activity of blood serum on 25%, content of Ig G - on 54,4%, Ig M - on 14,6 %, that indicated to the inefficiency of antigen administration and became the basis for termination of hyperimmunization cycle. Immunobiological reaction of bull-producers which were hyperimmunized by the suggested scheme differed from reaction of animals which were hyperimmunized by the production scheme: content of total protein increased on 10,1%, protein content - on 27,3 %, content of lg G - on 24,8 %, lg M - in 23,7%

It was established that in blood serum of pigs vaccinated against leptospirosis there was an authentic increasing of alanine aminotransferase, alkaline phosphatase and gamma-glutamyltransferase that made proved the presence of residual reactogenicity of biological preparations. In course of the experiment there were used 4 groups of swine which were administrated with 4 types of vaccines: 1. polyvalent vaccine produced in Belarus; 2. experimental thiosulfate vaccine in which there was used 30% sodium solution; 3. experimental emulsified vaccine; experimental emulsified + sodium + thiosulfate vaccine.

Influence of polyphepan with lactulose on dynamics of hematological parameters for the preventive maintenance of gastroenteritis of piglets was studied in the conditions of the Republic of Belarus. Research results showed that application of the presented preparation provided normalization of haemotological parameters of blood, reduction of a level of organism intoxication that was characterized by lowering of concentration lipid peroxidation components.

Comparative effectiveness of application of SV-1 and SV-2 sorbents for preventive treatment of abomazoenterite of 1-2 month old calves was studied in the conditions of the Republic of Belarus. Research results showed that application of SV-1 and SV-2 sorbents rendered a positive influence on hematological and biochemical blood indexes, in particular, there was an increasing of hemoglobin level, albumins content, alkaline reserve, calcium and phosphorous in blood serum. They also rendered the stimulatory effect on natural resistance. The prophylactic effectiveness in experimental group was 100%, in a control one – 60%.

Determination of the efficiency of young fat stock feeding with wet rolled maize grain which was preserved by means of different preparations was realized in the conditions of the Republic of Belarus. The following preserving agents were applied: HV-2 (by- product of amino-formaldehyde resin); AIV 3 Plus (mixture of 62% formic acid and 24% ammonium formate; KEMIRA, Finland); carbamide (30 kg/t) with 40 g/t of complex mineral supplement. Feeding of young fat stock with rolled maize grains preserved by NV-2 promoted the increasing of nutrient digestibility on 1,5-5,0%, nitrogen use - on 2% in comparison with control. Application of rolled maize grain preserved with AIV-3Plus and carbamide with the complex mineral supplement made it possible to increase the digestibility of nutrients on 1,0-4,8% and nitrogen use - on 1,6-1,7%. Introduction of rolled maize grain preserved with NV-2 into rations of bull calves increased their productivity on 4,7% (P less than 0,05) with the simultaneous lowering of forage expenses on 1,9%. Application rolled maize grains preserved with AIV-3Plus made it possible to increase daily average weight gain on 3,8% and lower the forages expenses on 2,6%. Use preserved with NV-2 and AIV-3Plus rolled maize grains in rations of young fat stock lowered the production cost price on 11,9% and 10,8% that proved the efficiency of the studied fodder

Evaluation of maize (Zea mays) silage prepared in combination with biological preserving agent Laktotim and calculation of its economic efficiency were realized in the conditions of the Republic of Belarus. Application of Laktotim rendered positive influence on forage dry matter content, on nutritive substance safety, and made it possible to increase the organoleptic indicators of silage. Use of the maize silage prepared with the preservative in a cow ration has positively affected their dairy efficiency. Cow milk yields have raised on 4,5%. Application of the preparation for silage making of green maize mass proved to be economically worth. The realized toxicological estimation of biological preserving agent Laktotim did not reveal any signs of preparation toxicity. Introduction of the preparation into mice stomach in a single maximum dose (25000 mg/kg; 12500; 5000 mg/kg of body weight), as well as application of the preparation in mixture with water in ratio which in 100 and 200 times exceeded the standard ratio in plant green mass, did not cause destruction of experimental animals. According to classifications of All-Union State Standard 12.1.007-76, the preparation Laktotim belongs to the4 class of danger - low-hazardous substances (LD50 over 5000 mg/kg)

Nowadays in connection with an interdiction for antibiotics use in animal feeding it is very actual to work out and apply new forms of probiotic preparations which should be as ecologically safe and harmless both to people and animal products. Probiotics make it possible to improve the digestibility of nutritive substances of forages, normalize metabolism, enrich with biologically active substances that promotes the increasing of their productivity. In the Republic of Belarus the probiotics are produced in the obviously insufficient quantity that creates problems of young stock livability. Influence of application of Akozil and Sublicin probiotics in diets of suckling calves on biochemical parameters of blood serum was analysed. It was stated that use of the analysed probiotics made it possible to decrease the level of urea and total cholesterol, to increase of the total protein content in blood serum of the experimental calves. There was noted the increasing of glucose level in blood serum of calves fed with Akozil. There was marked the tendency of more optimal level of calcium and phosphorus in calves having Sublicin in their diets. The obtained results testified the normalization of metabolic processes taking place in calves which were fed by the mentioned above probiotics

There was realized the estimation of influence of application of biometals in a ration of chickens-broilers of different age on the content in their blood macro-and microelements. It is possible to make the conclusion, that in blood of chickens-broilers the tendency to decrease with the years concentration of calcium and magnesium, to increase of level of phosphorus, zinc and copper, and also increase the content of cobalt with 23-day age to 33-day and its insignificant decrease by 45 day of a life of chickens takes place. At the same time application in a ration of chickens-broilers of biometals sodium-ferrum-ethylenediaminetetraacetate (Na-Fe-etda) from calculation of 2,5 mg/kg, sodium-zinc-ethylenediaminetetraacetate (Na-Zn-etda) - 5,5 mg/kg, sodium-cobalt- ethylenediaminetetraacetate (Na-Co-etda) - 5,5 mg/kg and sodium-cuprum-ethylenediaminetetraacetate (Na-Cu-etda) - 3,5 mg/kg of body weight in the basic weight led to small increase of level macro- and microelements in blood of chickens-broilers and did not render negative influence on safety and a gain of body weight of chickens in trial group. Data on concentration of macro- and microelements in blood and a body weight gain of chickens-broilers are presented. | Целью работы является оценка влияния применения биометаллов в рационе цыплят-бройлеров разного возраста на содержание в их крови макро- и микроэлементов. Можно сделать заключение, что в крови цыплят-бройлеров имеет место тенденция к снижению с возрастом концентрации кальция и магния, повышению уровня фосфора, цинка и меди, а также увеличению содержание кобальта с 23-дненвного возраста к 33-дневному и незначительному снижению его к 45-му дню жизни цыплят. В то же время применение в рационе цыплят-бройлеров биометаллов натрий-ферум-этилендиаминтетраацетат (Na-Fe-etda) из расчета 2,5 мг/кг, натрий-цинк-этилендиаминтетраацетат (Na-Zn-etda) - 5,5 мг/кг, натрий-кобальт-этилендиаминтетраацетат (Na-Co-etda-) - 5,5 мг/кг и натрий-купрум-этилендиаминтетраацетат (Na-Cu-etda) - 3,5 мг/кг живой массы в основной своей массе приводило к небольшому повышению уровня макро- и микроэлементов в крови цыплят-бройлеров и не оказывало негативного воздействия на сохранность и прирост живой массы цыплят опытной группы. Представлены данные по концентрации макро- и микроэлементов в крови и прирост живой массы цыплят-бройлеров.

In the conditions of the Republic of Belarus there was studied the pathology and biology of cattle capillariasis. Capillariosis invasion rendered a substantial invasion on blood morphological composition which was revealed in lowering of erythrocytes and hematoglobulin, as well as in increasing of leukocytes quantity. There was noted the lowering of the natural resistance and immune reactivity. There were stated substantial deviations in protein metabolism which was expressed in lowering of total protein, quantity of albumins, changing of albumin and globulin coefficient, increasing of globulins. In the conditions of blood biochemical analysis of infesrated animals there was stated a disorder of vitamin and mineral metabolism; increasing of alanine aminotransferase and aspartate aminotransferase enzymes activity. Development of Capillaria bovis was realized in a direct line. In the natural habitat development of Capillaria bovis eggs varied from two up to three months.

In the conditions of the Republic of Belarus there was studied the nonspecific resistance of lambs of Latvian Black Face breed during the colostrum and milk fed period of their growth development. In course of the experiment there were used 10 newborn lambs. Analysis of resistance was realized in accordance with a scheme: before colostral milk sucking; in 6, 12, 24 hours; in 3, 7, 14, 21 days; in 1, 2 and 3 months after birth. In lambs during the colostrum and milk fed period of growth there were marked two critical periods, which were characterized by low indicators of resistance: before colostral milk uptake and at 7-21 day-old age. Increasing of leukocytes, bactericidal, lysozymic activity of blood serum, phagocytal activity of leukocytes, crude protein and antibodies in the first days of lamb life took place due to their entrance in blood with colostral milk, just because at this time the intestines wall possessed ability to transit in native condition the antibodies, enzymes, and leukocytes. At 7-21 day-old age in connection with reduction of these factors quantity of in colostral milk, and also due to reduction of intestines capacity, their was stated that their entrance in blood of newborn lambs decreased, but at 3 month old age of lambs there were stated to form internal protective factors of resistance because of which their quantity in blood started to increase.