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Capacitation-like membrane changes and prolonged viability in vitro of equine spermatozoa cultured with uterine tube epithelial cells
1993
Ellington, J.E. | Ball, B.A. | Blue, B.J. | Wilker, C.E.
Reliable capacitation of equine spermatozoa has been a major obstacle in the development of equine in vitro fertilization. Experiments were done to compare in vitro capacitation of equine spermatozoa by use of heparin/caffeine, calcium ionophore, uterine tube epithelial cell (UTEC)-conditioned medium, and direct culturing of spermatozoa with UTEC (coculturing). Capacitation-like changes, as determined by chlortetracycline membrane staining patterns, developed with UTEC-conditioned medium and coculturing, equivalent to that with calcium ionophore. Both of these treatments induced more (P < 0.05) capacitation-like changes than did the control, a modified Tyrode's medium. More (P < 0.05) spermatozoa were viable after 24 hours of UTEC coculturing than in the control incubation.
Show more [+] Less [-]Detection of Actinobacillus pleuropneumoniae in the porcine upper respiratory tract as a complement to serological tests
1993
Sidibe, M. | Messier, S. | Lariviere, S. | Gottschalk, M. | Mittal, K.R.
Effects of Pasteurella haemolytica culture supernate on bovine tracheal smooth muscle
1993
Belanger, A. | Harris, W.H. | Yamashiro, S.
