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Comparison of Some Bacterial Identification Methods Full text
2019
Gulaydin, Ozgul | Eki̇n, Ismail Hakki | Oztürk, Cihat | Ilhan, Ziya | Ogun, Erdal
In this study, three different methods were compared for the identification of some Gram-positive and Gramnegative reference bacteria. Material and Methods: For this purpose, the identification accuracy rates of Enterococcus faecalis, Enterococcus faecium, Streptococcus agalactiae, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Serratia marcescens were analysed by conventional bacteriological methods, commercial bacterial identification test kit (Microgen™ ID) and automated bacteria identification system (BD Phoenix 100™).
Show more [+] Less [-]Molecular identification of Rusa timorensis (javan rusa) and Rusa unicolor (sambar deer) based on mitochondrial cytochrome c oxidase subunit I (COI) Full text
2021
Fatin Nabilah A. | Nurain Izzati S.
Locally, venison is considered as a premium and exotic meat, as it is not commonly found in fresh food markets and grocery stores. Despite its limited availability, demand is always high in its niche market, especially during festive seasons which highly escalate the price. However, as an expensive delicacy, deer meat is highly susceptible to fraudulent substitution and adulteration. Authentic deer meat are currently only recognized by consumers based on their own experience, meat texture, and taste which can be quite subjective. To assist in authenticating local deer meat in the market and protect consumers from fraudsters, Polymerase Chain Reaction (PCR) analysis can be carried out to distinguish between venison and other animal meat and products. Farmed venison in Malaysia are mostly from the species Rusa timorensis while Rusa unicolor is bred in the wild. Here, we detailed a newly developed conventional PCR method that is able to detect R. timorensis and R. unicolor based on partial mitochondrial cytochrome c oxidase subunit I (COI) in a single run, thus providing a simple and more accurate alternative in venison authentication.
Show more [+] Less [-]Multi-locus exon-primed intron crossing (EPIC) primer design for regional birds and algorithm design for a combination of introns Full text
2018
Kumakura, K. (Rakuno Gakuen University, Ebetsu, Hokkaido (Japan). School of Veterinary Medicine, Laboratory of Radiation Biology) | Osanai, Y. | Osa, Y. | Fujii, K. | Asakawa, M. | Ushiyama, K. | Onuma, M. | Igarashi, H. | Endoh, D.
Universal nematode detection by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) of purified nematode nucleic acids Full text
2018
Yoshino, T. (Rakuno Gakuen University, Ebetsu, Hokkaido (Japan). School of Veterinary Medicine, Laboratory of Parasitology) | Asakawa, M. | Osa, Y. | Kaneko, M. | Endoh, D.
Ultra-fast detection and differentiation of Brucella genus bacteria, B. abortus, B. melitensis, and B. suis, using multiplex convection polymerase chain reaction Full text
2019
Song, K. ((Ahram Biosystems Inc., Seoul (Korea). R&D center), (Kyung Hee University, Seoul (Korea). Department of Life and Nanopharmaceutical Sciences)) | Li, Z. | Kim, T.H. | Wang, X. | Bu, Z. | Li, S. | Hwang, H.J. | Kim, J.H.
