BACKGROUND: Vanadium and zinc are identified as low-concentration elements in the body of living organisms with a wide range of activities. Their insulin-like activity, through regulating the metabolism of carbohydrates, lipids, and eliminating the secondary symptoms of the disease, clearly demonstrates the ability of these elements to improve diabetes.OBJECTIVES: The present study aimed to evaluate the effect of vanadium-zinc complex on the structural, functional, and oxidative stress changes in liver tissue in adult diabetic male rats.METHODS: Herein, we recruited 40 adult male rats with the same weight range. They were randomly divided into four groups of 10, namely control, diabetic, healthy mice receiving vanadium-zinc complex, diabetic mice receiving vanadium-zinc complex) and received this combination at the rate of 10 mg / kg once a day for 60 days by oral gavage. At the end of the course, following blood sampling, part of the liver tissue was removed from the body to measure oxidative stress and the rest for stereological and histological studies. The serum isolated from the animals was also used to measure liver tissue functional enzymes (aspartate aminotransferase, alkaline phosphatase, alanine aminotransferase).RESULTS: The results of this research revealed that vanadium-zinc complex has no side effects on liver tissue in most case, but can greatly prevent structural damage to liver tissue by lowering blood glucose levels in diabetic rats and improving oxidative stress.CONCLUSIONS: Vanadium-zinc complex can be utilized with a certain mechanism in order to control blood sugar and inhibit oxidative stress. It could be regarded as an appropriate approach to preventing liver damage following chronic hyperglycemia in diabetic patients.