The study was conducted in 18 clinically healthy male dogs of about 1 year of age, weighing up to 12 kg and divided into three groups of 6 animals each. Bupivacaine alone, bupivacaine + hyaluronidase and hyperbaric bupivacaine were given epidurally at lumbosacral space @2 mg/kg. bwt. with a maximum dose of 3 ml in the animals of groups I, II and III, respectively. A significant increase (P0.01) in rectal temperature, heart rate and respiration rate was recorded at initial intervals of observation in all the groups and thereafter these values follow a declining trend in all groups consistently and reached to almost base line value at 120 min. of observation The alterations of glucose, serum urea nitrogen, aspartate aminotransferase and creatinine estimated at 1hr, 2hrs, 4hrs and 24 hrs were remained within physiological limits in all the groups.Therefore induction of regional anaesthesia with bupivacaine and its combination revealed no permanent alteration in the biochemical and physiological parameters hence it is safe and satisfactory anaesthesia.

A study was carried out in 2161 quarter milk samples of 550 cows in Durg district Chhattisgarh. Out of 550 animals, 385 (70%) animals were found to be positive for sub clinical mastitis (SCM) by Modified White Side Test (MWST), 432 (78.54%) by Modified California Mastitis Test (MCMT) and 462 (84%) by somatic cell count (SCC). The quarter wise prevalence of sub clinical mastitis was 47.99%, 55.25% and 60.90% by MWST, MCMT and SCC respectively. Prevalence of blind teats was 1.77%. prevalence was highest during second and third lactations and at 5 and 6 years of age. Infection rate was higher during early and late stages of lactation. HF and Jersey cross bred cows were more susceptible than indigenous cows. Microorganisms isolated were predominantly Staphylococci. ABST revealed sensitivity to cefotaxime whereas most of the isolates were resistant to ampicillin.