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Serological survey of leptospirosis, brucellosis, and lentivirus in herds of small ruminants in Recôncavo Baiano, Bahia, Brazil
2021
Rodrigo Rezende Mires de Carvalho | Lucas Nogueira Paz | Carla Silva Dias | Giselle Almeida Nocera | Augusto José Pereira de Mesquita | Melissa Hanzen Pinna
A serological survey was carried out to assess the frequency of leptospirosis, small ruminants lentivirus (SRLV), and brucellosis in small ruminant herds in the Recôncavo Baiano, State of Bahia, Brazil, from February to December 2017. In four goat herds, 125 animals were tested for SRLV and leptospirosis, while in five sheep herds, 378 animals were tested for leptospirosis, brucellosis, and SRLV. Regarding leptospirosis, MAT detected 80% of goats and 15.34% of sheep seroreactive. Reactivity was most frequent to serogroups Autumnalis and Grippotyphosa in goats and sheep, respectively. Regarding SRLV, 8.8% of goats and 0.79% of sheep were reactive. Search for anti-B. ovis antibodies revealed 0.52% reactivity. In sheep, three animals showed simultaneous seroreactivity for SRLV and leptospirosis, while one animal had a serological response for brucellosis and leptospirosis. In goats, simultaneous seroreactivity for SRLV and leptospirosis was observed in only one animal. Leptospirosis was the most frequent of the three infectious diseases in investigated herds.
Show more [+] Less [-]Occurrence of Mycoplasma hyopneumoniae in slaughter pigs from Southern Brazil
2019
Violetta Dias Pacce | Natasha Rodrigues de Oliveira | Sérgio Jorge | Odir Antônio Dellagostin
Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia (EP), a disease that is highly prevalent and globally distributed, causing significant economic losses to the swine industry. Disease progression is characterized by reduced feed conversion and the development of lung lesions. Considering the limited information about the epidemiology of EP in Southern Brazil, the main objective of this study was to determine the occurrence of M. hyopneumoniae in swine lung samples and to evaluate the scores of lung lesions caused by local strains. A total of 120 samples was randomly collected and processed. DNA was extracted from lung tissue to perform nested-PCR and lungs were inspected to evaluate the presence of the pneumonia-like gross lesions of M. hyopneumoniae. The results showed 95.8% positive samples, while the lung lesion score analysis showed suggestive lesions in 60% of samples. The detection of positive samples in nested-PCR was associated with the presence of pneumonia-like gross lesions (P < 0.01). The results demonstrate a high occurrence of EP in slaughter pigs from southern Brazil.
Show more [+] Less [-]Use of serological diagnostic techniques in the control and eradication of caprine arthritis encephalitis: an update
2015
Jamili Maria Suhet Mussi | Aurora Maria Guimarães Gouveia | Adriana Cortez | Andrey Pereira Lage | Alessandro de Sá Guimarães | Marcos Bryan Heinemann
Caprine arthritis encephalitis (CAE) is a chronic disease caused by a small ruminant lentivirus (SRLV), which causes significant losses in goat breeding. The actual state of animal infection with SRLV is difficult to determine due to a complex pathogenesis of the virus, including factors such as delayed or intermittent seroconversion in serological tests. Several serological techniques are available for disease diagnosis, such as screening or confirmation tests, which are different in sensitivity and specificity. Regarding the choice of the test to be applied, availability of commercial immunoreagents, team training, antigen used, and cost of techniques must be considered. This review presents the serological methods available for use in different stages of CAE control and eradication programs, and management measures to be adopted in conjunction with serological diagnosis of the disease.
Show more [+] Less [-]Detection of rabies virus antigen by direct immunoperoxidase and fluorescent antibody test in experimentally inoculated mice, sacrificed in assymptomatic and agonizing state
1990
Megid, J. | Ito, F.H.
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescense of rabies
1991
Richtzenhain, L.J. | Ito, F.H. | Jerez, J.A. | Mavatari, A.R.Y. | Albas, A.