Adult Beagles were used to evaluate clinical signs and serologic response after inoculation with, or exposure to, Borrelia burgdorferi. An indirect immunofluorescent assay (IFA) and 2 ELISA were used to monitor the serologic response to B burgdorferi. Feeding infected ticks on 4 dogs (group 1) failed to cause seroconversion, and SC inoculation with 500 organisms caused minimal seroconversion in 2 of 4 dogs (group 2). At 56 days, approximately 3.01 X 10(8) B burgdorferi organisms were injected IV into group-1 dogs, and intraperitoneally into group-2 dogs. A control group of 4 dogs (group 3) had noninfected ticks feed on them, and then were given IV injection of physiologic saline solution. Increases in immunoglobulin M (IgM) titers were detected in 2 of 4 group-2 dogs approximately 7 days after the initial exposure. These titers returned to negligible values 20 days later. Immunoglobulin G titers increased approximately 10 days after the initial exposure and were mildly increased 56 days later, when dogs were exposed a second time. Both the IV and intraperitoneal injections (second exposures) resulted in increased IgM titers, which in both groups eventually returned to preexposure values after approximately 2 months. Immunoglobulin G titers increased within a week after the second exposure, and in 3 dogs monitored for 8 months, returned to negligible values after the 8-month period. One control dog had a slightly increased IgG titer 24 days after the second inoculation. The possibility of urine transmission is suggested. Clinical status, hemograms, serum biochemical profiles, ECG and results of urinalyses remained normal throughout the study. Borrelia burgdorferi was not isolated from either the blood or urine of these dogs. Gross or microscopic pathologic changes were not detected on necropsy.

Attempts of these studies were made to investigate the nonspecific congenital focal accumulation of etcodermal glial cells in the brain of normal piglets. The brain samples were taken from 1-, 10-, 20-, 35-, 45- and 70-day-old piglets from a SPF-pig farm and three model pig farms. Occurrences of neuroglial cell foci(NCF) on the brain were observed with light microscope. Appearance degrees of the congenital NCF on 10 to 16 cross section slides per a piglets brain were tentatively designed on a scale from deg. + to +++ by NCF number: +, less than 20 of NCF number; , 21-40 of NCF number: +++ , more than 41 of NCF number. NCF in the brain were obseved mainly on the cerebrum. Regions of higher frequencies on the cerebrum were ordered as subependymal layers of the lateral ventricles, peripheral regions of lateral ventricles in the white matter and some neuron layers under the molecular layer of the gray matter. But NCF were not observed in the cerebellum, pons, medulla oblongata and spinal cords. On the subependymal layers of the lateral ventricles, NCF were observed in 100% of 27 piglets, and appearance degree of was observed in 10 piglets (37.0%), ++ in 10 piglets (37.0%) and + in 7 piglets (26.0%) of 27 piglets, respectively. On the white matter of the cerebrum, NCF were observed in 25 piglets (92.6%) of 27 piglets, and appearance degree of +++ was observed in 3 piglets (11.1%), in 13 piglets(48.2%), + in 9 piglets (33.3%) and- in 2 piglets (7.4%) of 27 piglets, respectively. On the gray matter of the cerebum, NCF were observed in 21 piglets (77.8%) of 27 piglets, and appearance degree of +++ was not observed, appearance degree of ++ was obseved in 6 piglets (22.2 %), + in 15 piglets (55.6%) and - in 6 piglets (22.2%) of 27 piglets, respectively. NCF tended to be converged appearance on some regions and tended to be decreased markedly from 35th day after birth, and the shapes of NCF were global or oval forms crowded by analogous shaped and stained cells in the empty spaces of the brain substrate or on one side of the blood vessels