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Quantitation of canine plasma von Willebrand factor antigen using a commercial enzyme-linked immunosorbent assay
1991
Johnstone, I.B. | Crane, S.
The purpose of this study was to evaluate a commercial enzyme-linked immunosorbent assay (ELISA) for human von Willebrand factor antigen (vWF:Ag) with respect to its potential value in quantitating the protein in canine plasma. The assay was a sandwich technique using F(ab')2 fragments specific for von Willebrand factor (vWF) and a peroxidase conjugated rabbit anti-vWF second antibody, with a microplate as the support surface. Canine plasmas were assayed by ELISA, and by Laurell electroimmunoassay (EIA), our reference methodology. The ELISA had a within-day variation of 1.21-4.44% and a between-day variation of 0.85-4.88% depending on the level of vWF:AG. The sensitivity of the assay was less than 0.1% vWF:AG. The range of vWF:AG concentrations in plasmas from 24 clinically normal dogs compared favorably with the range for the same plasmas when assayed by EIA (ELISA = 60-152% of normal; EIA = 50-142% of normal). In 121 canine plasmas with vWF:AG concentrations (as assessed by EIA) ranging from undetectable levels (< 6% of normal) to 142% of normal, there was good correlation with measurements made by ELISA (correlation coefficient = 0.835). It was concluded that this commercial ELISA technique could be used to provide reliable, same-day measurements of canine plasma vWF:AG. Since it requires no special equipment other than a microplate reader and washer it is particularly suitable for laboratories lacking the electrophoretic expertise or equipment required for EIA.
Show more [+] Less [-]Fine needle aspiration biopsy for the diagnosis of fatty liver in cattle
1991
Hwang, B.T. | Han, H.R. (Seoul National Univ., Suwon (Korea Republic). Coll. of Veterinary Medicine)
Clinical and bacteriological studies on infectious atrophic rhinitis of swine
1991
Kim, B.H. | Tak, R.B. (Kyungpook National Univ., Taegu (Korea Republic). Coll. of Veterinary Medicine) | Cho, G.J. (Korea Horse Affairs Association, Equine Health Laboratory) | Jang, H.K. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
A study on production of early pregnancy diagnostic kid in cattle, 2; Production of early pregnancy diagnostic kit.
1991
Kang, C.B. | Lee, H.J. | Choe, S.Y. (Gyeongsang National Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine)
Use of enzyme-linked immunosorbent assay (ELISA) for detection of toxoplasmosis in dogs
1991
Suh, M.D. | Joo, H.D. | Lee, B.H. (Gyeongsang National Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine)
A study on production of early pregnancy diagnostic kit in cattle, 1; Production of polyclonal antibody to progesterone and removal of anti-bovine serum albumin antisera
1991
Kang, C.B. | Lee, H.J. | Choe, S.Y. (Gyeongsang National Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine)
Studies on the plasma progesterone concentrations for pregnancy diagnosis in Cheju native mare
1991
Chang, D.J. (Cheju Vocational Junior Coll., Cheju (Korea Republic)) | Yang, K.C. | Kim, J.K. (Cheju National Univ., Cheju (Korea Republic). Coll. of Agriculture) | Kang, B.K. | Choi, H.S. | Son, C.H. (Chonnam National Univ., Kwangju (Korea Republic). Coll. of Veterinary Medicine)
Techniques for laboratory diagnosis of cryptosporidiosis
1991
Noh, J.W. | Kang, D.W. | Jang, D.H. (Seoul National Univ., Suwon (Korea Republic). Coll. of Veterinary Medicine) | Kang, Y.B. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescense of rabies
1991
Richtzenhain, L.J. | Ito, F.H. | Jerez, J.A. | Mavatari, A.R.Y. | Albas, A.
