Studies were conducted to determine the utility of lysate antigens for rapidevaluation of the local entero-3 vaccine, antigens were prepared from cell culturesinfected with bovine rota virus (BRV) and bovine corona virus (BCV) as well asfrom Enterotoxigenic E. coli strain K99. Prepared antigens were tested with fieldserum samples collected from both late pregnant entero-3 vaccinated cows and their offsprings using different serological assays including: microagglutination test,indirect ELISA and immunofluorescent antibody technique. Results of this endeavorwere correlated to that of the standard virus neutralization test. The locally preparedantigens were proved useful for vaccine evaluation. Moreover, these antigens arerecommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV and E. coli.

African swine fever (ASF) is an infectious disease of domestic and wild pigs for which there is no vaccine in the world. A proper surveillance of viral activity and a timely response to ASF outbreaks depend upon the rapid diagnosis of ASF viral infection. Internationally prescribed enzyme-linked immunosorbent assay (ELISA) is a fast, sensitive test routinely used in the diagnosis of the ASF. However, inactivated whole ASF virus antigen used in this test is a tedious to prepare and has a risk of outside exposure of infectious virus by laboratory accident during the preparation.