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Experimental transmission of bovine leukemia virus in cattle via rectal palpation
2006
Kohara, J.(Hokkaido. Animal Research Center, Shintoku (Japan)) | Konnai, S. | Onuma, M.
We examined whether Bovine leukemia virus (BLV) was transmitted by rectal palpation using a common sleeve between a BLV-infected cow and BLV- negative steers. Three of four steers developed antibodies against BLN as determined by agar-gel immunodiffusion (AGID) test between 7 to 10 weeks after the first rectal palpation using common sleeves from BLV-infected cow. In the steers, BLV proviral DNA were detected by PCR 1 to 5 weeks earlier than detection of the antibodies by the AGID test. Our experiments demonstrated that rectal palpation is a potential cause of BLV spread in herds and that detection of BLV proviral DNA in cattle by PCR is useful screening test for early diagnosis of BLV infection.
Show more [+] Less [-]Progesterone concentrations measured by radioimmunoassay in blood plasma and in fat-free milk of Gir breed cows (Bos indicus). Determinations during oestrous cycle. Early pregnancy diagnosis
1990
Madureira, E.H. | Barnabe, R.C. | Pinto, P.A. | Barnabe, V.H. | Martins Sobrinho, E.O.
