The study was conducted in 18 clinically healthy male dogs of about 1 year of age, weighing up to 12 kg and divided into three groups of 6 animals each. Bupivacaine alone, bupivacaine + hyaluronidase and hyperbaric bupivacaine were given epidurally at lumbosacral space @2 mg/kg. bwt. with a maximum dose of 3 ml in the animals of groups I, II and III, respectively. A significant increase (P0.01) in rectal temperature, heart rate and respiration rate was recorded at initial intervals of observation in all the groups and thereafter these values follow a declining trend in all groups consistently and reached to almost base line value at 120 min. of observation The alterations of glucose, serum urea nitrogen, aspartate aminotransferase and creatinine estimated at 1hr, 2hrs, 4hrs and 24 hrs were remained within physiological limits in all the groups.Therefore induction of regional anaesthesia with bupivacaine and its combination revealed no permanent alteration in the biochemical and physiological parameters hence it is safe and satisfactory anaesthesia.

The canines affected with skin affections were subjected to blood biochemical profile assay. The affections included dermatitis, sarcoptic mange, demodectic mange, eczema, flea allergy dermatitis, skin allergy and bacterial dermatitis. The blood profile of serum iron, triglyceride, cholesterol, uric acid, urea, creatinine, lactate dehydrogenase, and gamma glutamyl transpeptidase were compared with the blood of healthy dogs. The serum iron, urea and creatinine were comparable in both the groups. It was concluded that stress of dermatosis reflected in enzyme (higher in GGT) and lipid profile (higher in triglyceride, cholesterol) of patients, while renal parameters (urea and creatinine) remain unaffected on account of skin ailments.