Feline parvovirus infection (FPV) is one of the serious diseases in Kittens that causes substantial morbidity and death. For the treatment of affected cats and the prevention of disease spread, early diagnosis of FPV infection is critical. To our knowledge, there have been no reports about the disease’s situation in Egypt’s Assiut province. As a result, the goal of this study was to find out how common FPV infection is among ill cats in this province. A total of 30 cats suspected of being infected with FPV were screened using an antigen rapid test to determine whether they were clinically suspicious. To determine the prevalence of FPV, each investigated cat’s age, sex, breed, season, lifestyle (whether kept indoors or outdoors), and immunization were all documented. Overall, 26.7% of examined cats were affected. FPV infection was more common in young, unvaccinated cats who lived outdoor. Epizootiological monitoring of the prevalence rate based on cat breeds and sex revealed no statistically significant differences. In terms of season, spring had the highest infection rate (57.1%), followed by winter (33.3%), and autumn (7.69%). The rapid one-step test is a useful diagnostic tool for detecting FPV, which was found in the research area’s cats.

Brucellosis is a major constraint to livestock production that still enzootic in livestock in many developing countries including Egypt. This study was conducted with the general objective of establishing the bacteriological status of bovine brucellosis in 15 governorates in Egypt during 2020-2021 to determine the circulating Brucella species on bacteriological and molecular basis. Clinical samples collected included milk or udder secretions, vaginal discharges, fetal membranes and stomach contents of aborted fetuses from dairy cows with history of brucellosis. In addition, lymph nodes (retropharyngeal, prescapular, prefemoral, internal iliac and supramammary) from carcasses of serologically positive animals were obtained from different localities for isolation and identification of Brucella organisms. A total of 136 Brucella isolates were recovered from cattle in different governorates, Egypt. These include, 107 isolates of Brucella melitensis biovar 3 identified on bacteriological and molecular basis from Aswan, Beheira, Beni Suef, Dakahlia, Damietta, Fayoum, Gharbia, Giza, Ismailia, Kafr El-Sheikh, Luxor, Monufia, Port Said, Qalyubia and Sharqia governorates. On the other hand, 29 Brucella abortus biovar 1 isolates were recovered from cattle from Beni Suef, Dakahlia, Damietta, Kafr El-Sheikh, Monufia, Port Said and Sharqia governorates. Molecular identification using primer sequences targeting IS711 gene confirmed Brucella on genus level. Multiplex PCR has amplified four fragments of 450bp, 587 bp, 1071 bp, and1682 bp characteristic for B. melitensis biovar 3, and three fragments of 450bp, 587 bp, and 1682 bp for B. abortus biovar 1. The identification of Brucella spp. in different farm animals of 15 Egyptian governorates highlights the dynamics and role of cattle in dissemination of Brucella infection all over the country. The obtained results indicate that the actual Brucellosis status during the years 2020 and 2021 refers to that B. melitensis biovar 3 and B. abortus biovar 1 are the prevalent types circulating in different Egyptian governorates.

Sarcocystis spp. is cyst forming protozoa that contains more than 200 species and belong to the phylum Apicomplexa. This study aimed to investigate the prevalence of Sarcocystis spp. in buffalo and sheep carcasses slaughtered in Egypt macroscopically and microscopically. For this purpose, a total of 400 buffalo and sheep carcasses were examined at Tanta abattoir, Egypt for the detection of Sarcocystis spp. from July 2020 to June 2021. The results revealed that the prevalence of macroscopic sarcocysts was 26.5% in slaughtered buffaloes and 0% in slaughtered sheep, while the prevalence of microscopic sarcocysts was 56% in slaughtered buffaloes and 80.5% in slaughtered sheep. The prevalence of sarcocysts in old buffaloes and sheep was higher than in young buffaloes and sheep. The most affected organs with microscopic sarcocysts were the oesophagus followed by the tongue, masseter muscle, skeletal muscles and finally heart. The obtained results confirmed that the examined buffaloes and sheep are infected with Sarcocystis species due to the abundance of final hosts, especially dogs and cats that encourage the spreading of infection by this protozoan parasite. Therefore, efficient cooking of buffalo meat is highly recommended before serving to humans.

There is an increasing demand for chicken meat products due to their high nutritive value, specific aroma and flavor, and cheap price compared to red meat. However, there is a clear lack of information on the residual content of toxic metals such as lead (Pb), cadmium (Cd), mercury (Hg), and trace elements such as zinc (Zn), and copper (Cu) in such products. Therefore, this study was undertaken to estimate the residual contents of Pb, Cd, Hg, Zn, and Cu in five chicken meat products including chicken burger, chicken fillet, chicken luncheon, chicken nuggets, and chicken panne. Moreover, estimated daily intakes (EDI), and the potential health risks of heavy metals due to consumption of such meat products were calculated for Egyptian adults and children. The achieved results indicated no detection of Hg in any sample. However, the other measured elements were detected in all examined chicken meat product samples at variable concentrations. The chicken burger had the highest metal concentrations. Several samples had higher Pb and Cd levels than the set maximum permissible limits. However, the calculated EDI, hazard ratio, and hazard index revealed no potential risks associated with the consumption of such chicken meat products among Egyptian adults and children.