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Development of enzyme linked immunosorbent assay for seromonitoring against hydropericardium syndrome (ELISA) virus
2003
Manzoor, R. | Kamal, T. | Khawaja, D.A. | Ismail, M.A. | Gill, Z.J. (Veterinary Research Inst., Lahore (Pakistan))
In this study ELISA was standardized for detecting antibodies against Hydropericardium Syndrome Virus. Factors like Antigen, Conjugate and incubation time were studied. Standardization was carried out by checker board titration method. Optimum incubation time for colour development was found to be 15 minutes while 50 mug/ml of antigen gave better results than 100 mug/ml. The conjugate dilution of 1:500 was observed to give satisfactory results as determined by regression analysis. Cut off value was determined by adding 2SDs and 3SDs in the mean optical density (OD) values of negative serum samples. It was found that mean OD+2SD (0.0684) resulted in better diagnostic sensitivity) and diagnostic specificity than mean OD+3SDs (0.0741).
Show more [+] Less [-]Evaluation of an indirect enzyme-linked immunosorbent assay for the detection of feline lentivirus-reactive antibodies in wild felids, employing a puma lentivirus-derived synthetic peptide antigen
2003
Van Vuuren, M. | Stylianides, E. (Pretoria Univ., Onderstepoort (South Africa). Veterinary Tropical Diseases Dept.) | Kania, S.A. | Zuckerman, E.E. | Hardy, W.D.
Comparison of complement fixation test, immunoblotting, indirect ELISA, and competitive ELISA for detecting antibodies to Mycoplasma mycoides subspecies mycoides small colony (SC) in naturally infected cattle from the 1995 outbreak in Botswana
2003
Marobela-Raborokgwe, C. (National Veterinary Laboratory, Gaborone (Botswana)) | Nicholas, R. | Ayling, R. | Bashiruddin, J.B.
Detection of antibodies to the Ehrlichia ruminantium MAP1-B antigen in goat sera from three communal land areas of Zimbabawe by an indirect enzyme-linked immunosorbent assay
2003
Kakono, O. | Hove, T. (Veterinary Science Faculty, Harare (Zimbabwe)) | Geysen, D. | Mahan, S.
Indirect enzyme-linked immunosorbent assay for the detection of antibody against Rift Valley fever virus in domestic and wild ruminant sera
2003
Paweska, J.T. | Smith, S.J. | Wright, I.M. | Williams, R. | Cohen, A.S. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Van Dijk, A.A. | Grobbelaar, A.A. | Croft, J.E. | Swanepoel, R. | Gerdes, G.H.
Pesquisa sorológica de anticorpos anti-Toxoplasma gondii em animais de produção do Estado de São Paulo, Brasil | Serological survey of antibodies to Toxoplasma gondii in food animals from São Paulo state, Brazil
2003
Luciana Regina Meireles | Andrés Jimenez Galisteo Jr. | Heitor Franco de Andrade Jr.
A toxoplasmose é uma das infecções parasitárias mais prevalentes no homem e no rebanho, e a sua transmissão tem sido usualmente atribuída à ingestão de carnes cruas ou mal cozidas, de animais de produção infectados, sendo que a taxa de infecção destes animais pode ser um importante índice preditivo da doença humana, de alta prevalência no Brasil e no estado de São Paulo. Estudando este aspecto de Saúde Pública, nós testamos amostras séricas de bovinos, caprinos, ovinos e frangos de corte de matadouros do Estado de São Paulo, para a presença de anticorpos IgG anti-Toxoplasma gondii por um ensaio imunoenzimático de fase sólida (ELISA). Nós encontramos anticorpos específicos em 31,00% (62/200) dos ovinos, 17,00% (34/200) dos caprinos e 11,00% (22/200) dos bovinos testados, sem positividade em 185 amostras de frangos de corte. Apesar de diferenças em hábitos alimentares de cada espécie, estas taxas de infecção foram menores em criações intensivas, relacionadas ao tipo de manejo da criação, o que pode reduzir a taxa de infecção por este parasita no rebanho. | Toxoplasmosis is one of the most prevalent parasitic infections of man and livestock, and its transmission has usually been attributed to ingestion of undercooked or raw meat from infected livestock, with the infection rate in those animals being an important risk predictor of human disease, high in Brazil and São Paulo State. Looking for this public health problem, we tested serum samples from cattle, goat, sheep and chicken from the State of São Paulo, Brazil, for IgG antibodies to Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA). Antibodies to Toxoplasma gondii were found in 31.00% (62/200) of sheep, 17.00% (34/200) of goat and 11.00% (22/200) of cattle, without positive sample in chicken. Despite differences in feeding habits of each species, the rate of infection of tested animals could be better attributed to livestock management methods, which improvement could reduce infection.
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