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Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido [Japan] and Far-Eastern Russia
2002
Goto, A. (Hokkaido Univ., Sapporo (Japan)) | Hayasaka, D. | Yoshii, K. | Mizutani, T. | Kariwa, H. | Takashima, I.
We compared the biological properties of Oshima 5-10 (tick-borne encephalitis [TBE] virus isolated in Hokkaido, Japan) and Sofjin-HO (Far-Eastern subtype TBE virus) including plaque formation, virus replication and virus protein synthesis in BHK-21 cell cultures to reveal strain differences. We also determined the complete nucleotide sequences of both strains and compared the deduced amino acid sequences. Plaques of Oshima 5-10 were smaller than those of Sofjin-HO. Virus titers in culture fluid of Oshima 5-10 were 1/100 of those of Sofjin-HO at 9 and 12 hr after infection. Less viral protein and RNA syntheses of strain Oshima 5-10 was observed than with Sofjin-HO. Genetic analysis revealed 1.4% of amino acids to differ with Sofjin-HO. No difference between the two strains was detected in the motif sequence of the viral enzyme, cleavage sites of viral protein or glycosylation sites of NS1.
Show more [+] Less [-]The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa
2002
Howell, P.G. (Pretoria Univ., Onderstepoort (South Africa). Equine Research Centre) | Groenewald, D. | Visage, C.W. | Bosman, A.-M. | Coetzer, J.A.W. | Guthrie, A.J.
