In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2aminoethyl-isothiouronium bromide (AET) and dextran (Dex), singly or in combination. To further standardize the assay, optimum concentration of AET-and/or Dex-treatment and incubation time for rosette forming cell (RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythrocyte-antibody (EAfc)- and erythrocyte-antibody-complement (EAC)- rosetting techniques. The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette (6.7 +- 2.4 %) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8 % Dex were formed as 37.3 +- 2.7 % and 45.1 +- 2.1 %, respectively. And the synergistic effects were noted no less than 66.5 +- 5.6 % when the KGRBC treated with 0.1M AET and 8 % Dex subsequently and rate of RFR did not change significantly between 3-24 hours incubation time at 4deg C, EA-and EAC-RFR were 23.3 +- 9.1 % and 23.1 +- 7.9 %, respectively. These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA-or EAC-rosette assay in cattle-PBL

The study was undertaken to obtain indirect values of volume of packed red cell (VPRC) without centrifugation, using the erythrocyte sedimentation rate (ESR) of diluted blood in dogs. ESRs of diluted blood using the diluent of autologous plasma in which formed numerous RBC-rouleau clumps, autologous serum in which formed a few RBC-rouleau clumps, and 5 % dextrose or 6 % sucrose solutions in which formed numerous RBC-aggregation clumps, were accelerated. But ESR of diluted blood using the 0.9 % saline, D-S, ACD-B, CDP or D-PBS solutions were sluggish, because erythrocytes were dispersed in these diluents. Reliable values of VPRC on the basis of the correlating regressive equation to the ESR could be derived from values of 60deg C-angled-micro-ESR/40 min in the mixture, four parts of 5 % dextrose solution and one part of whole blood. In the ESR values of diluted blood with low ratio, 1 : 1-3 : 1, 60deg C-micro-ESR was higher than 60deg C-Wintrobe-ESR. But, in the diluted blood with high ratio, 4 : 1-5 : 1, there was no different ESR values. For an aid of practical use, authors suggested a list of the 60deg C-micro-ESR/40 min in the diluted blood with equivalent VPRC of whole blood

The present study was conducted in order to get the normal hematologic values of Korean Jindo dogs. Blood samples were taken from 328 (male 92, female 236) healthy Jindo dogs in Jindo area. The mean values of red blood cell (RBC) count, hemoglobin (Hb) content and packed cell volume (PCV) in the age group of less than one year were 6.77(male 7.44, female 6.76)X10** (6)/ul, 13,14(male 13.53, female 12.99)g/100ml and 43.28(male 44.47, female 42.79)ml/100ml, respectively, whereas the RBC count, Hb content and PCV in the age group of one year and more were 7.42(male 7.44, female 7.42)X10** (6)/ul, 14.98(male 15.56, female 14.76)g/100ml and 47.18(male 48.43, female 46.71)ml/100ml, respectively. The values of RBC count, Hb content and PVC appeared to be higher in the male than in the female and it increased with aging in the age group of less than one year. The mean values of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin content (MCHC) in the age group of less than one year were 63.93(male 65.40, female 63.30)fl, 19.41(male 19.90, female 19.22) pg and 30.36(male 30.43, female 30.36)g/100ml respectively, whereas the MCV, MCH and MCHC in the age group of one year and more were 63.58(male 65.09, female 62.95)fl, 20.19(male 20.91, female 19.89) pg and 31.75(male 32.13, female31.60)g/100ml respectively. The values of MCV, MCH and MCHC appeared to be higher in the male than in the female. No differences were found in the MCV and MCH between age groups. The MCHC appeared to be higher in older age group. Mean platelet count in the age group of less than one year was 377,391(male 398,778, female 368,721)/ul, whereas mean platelet count in the age group of one year and more was 354,657(male 373,660, female 347,512)/ul. The platelet count appeared to be higher in the male than in the female and especially lower in the age group of three years and more (305,513/ul) than in the other age groups

The proteins of the ruminant erythrocyte membranes were analysed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate, and their relations to the slow erythrocyte sedimentation rate (ESR) of the ruminants were investigated by treating the erythrocytes with proteinases such as trypsin, chymotrypsin and pronase, and glycosidases such as neuraminidase and galactosidase. Protein content in the erythrocyte membrane was 2.85 +- 0.28 in human, 3.60 +-0.41 in Korean cattle, 3.71 +- 0.36 in Holstein, 4.13 +-0.83 in Korean native goat and 3.94 +- 0.56 mg/ml in sheep, showing higher in ruminant animals than in human (p0.01). Although the general protein profiles of the ruminant erythrocyte membranes were almost similar to that of human, all the ruminant erythrocyte membranes showed one additional protein band, called band- Q in the previous report on proteins of bovine erythrocyte membrane, which migrated electrophoretically to the mid position between band-2 and band-3 in human erythrocyte membranes. The glycoprotein profiles of ruminant erythrocyte membranes revealed by periodic acid Schiff (PAS) stain showed a marked difference from that of human. The PAS-l (glycophorin) and PAS-2 (sialoglycoprotein) present in human erythrocyte membranes were almost absent from the ruminant animals. Instead, a strong PAS-positive band near the origin of the electrophorograms, which was named as PAS-B in the previous report on proteins of bovine erythrocyte membranes, was shown in the ruminant animals except sheep. In addition, the erythrocyte membranes of Korean native goat and sheep showed a moderate PAS-negative band near the tracking dye of the electrophorograms, which was named as PAS-G in this study. In the erythrocyte treated with the enzymes, the migration of each protein fracture of erythrocyte membranes in response to each enzyme was diverse according to different species or breed of ruminant animals. Among others, band-Q present in ruminants was slightly or moderately decreased by trypsin-, chymotrypsin-, and pronase- treatments of the erythrocytes, but not only in sheep