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Electrophoretic analysis of the major proteins of race horse erythrocyte membrane: Their relation to fast erythrocyte sedimentation rate.
1991
Bahk, Y.W. (Kwangju Health Junior Coll., Kwangju (Korea Republic). Dept. of Clinical Pathology) | Lee, S.Y. (Korea Horse Affairs Association, Kwachon (Korea Republic). Equine Health Research)
Studies on red cell fragility and glutathione peroxidase activities in Korean native cattle of Chonbuk region
1990
Cho, J.H. | Lee, S.H. (Chonbuk National University, Chonju (Korea Republic). Department of Veterinary Medicine)
Indirect calculation for volume of packed red cell (VPRC) by means of erythrocyte sedimentation rate (ESR) of blood diluted with isotonic dextrose solution in cattle
1990
Lee, B.W. | Min, B.M. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine) | Bahk, Y.W. (Kwangju Health Junior College, Kwangju (Korea Republic). Department of Clinical Pathology)
Electrophoretic analysis of the major proteins of ruminant erythroctye membrane: Their relation to slow erythrocyte sedimentation rate
1989
Lee, B.W. (Chonnam National Univ., Kwangju (Korea R.). Coll. of Veterinary Medicine) | Bahk, Y.W. (Kwangju Health Junior Coll., Kwangju (Korea R.). Dept. of Clinical Pathology)
The proteins of the ruminant erythrocyte membranes were analysed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate, and their relations to the slow erythrocyte sedimentation rate (ESR) of the ruminants were investigated by treating the erythrocytes with proteinases such as trypsin, chymotrypsin and pronase, and glycosidases such as neuraminidase and galactosidase. Protein content in the erythrocyte membrane was 2.85 +- 0.28 in human, 3.60 +-0.41 in Korean cattle, 3.71 +- 0.36 in Holstein, 4.13 +-0.83 in Korean native goat and 3.94 +- 0.56 mg/ml in sheep, showing higher in ruminant animals than in human (p0.01). Although the general protein profiles of the ruminant erythrocyte membranes were almost similar to that of human, all the ruminant erythrocyte membranes showed one additional protein band, called band- Q in the previous report on proteins of bovine erythrocyte membrane, which migrated electrophoretically to the mid position between band-2 and band-3 in human erythrocyte membranes. The glycoprotein profiles of ruminant erythrocyte membranes revealed by periodic acid Schiff (PAS) stain showed a marked difference from that of human. The PAS-l (glycophorin) and PAS-2 (sialoglycoprotein) present in human erythrocyte membranes were almost absent from the ruminant animals. Instead, a strong PAS-positive band near the origin of the electrophorograms, which was named as PAS-B in the previous report on proteins of bovine erythrocyte membranes, was shown in the ruminant animals except sheep. In addition, the erythrocyte membranes of Korean native goat and sheep showed a moderate PAS-negative band near the tracking dye of the electrophorograms, which was named as PAS-G in this study. In the erythrocyte treated with the enzymes, the migration of each protein fracture of erythrocyte membranes in response to each enzyme was diverse according to different species or breed of ruminant animals. Among others, band-Q present in ruminants was slightly or moderately decreased by trypsin-, chymotrypsin-, and pronase- treatments of the erythrocytes, but not only in sheep
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