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Isolation of enteropathogenic Escherichia coli, thermophilic Campylobacter and Salmonellae from scouring piglets
1988
Lee, J.H. | Cho, H.T. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Agriculture) | Kim, Y.H. | Kang, H.J. | Cha, I.H. (Kyongnam Animal Health Laboratory, Chinju (Korea R.))
This study was conducted to isolate etiological agents from the 103 scouring piglets in Kyongnam area and also carried out antimicrobial drug susceptibility test and epidemiogical survey. The incidence of scouring piglet was most prevalent in 81.6% at the age of 2 to 4 weeks after birth, while the rate was less than 10% at the age of 5 to 6 weeks and under 1 week after birth. When comparing the isolation frequency of each etiological agent, enteropathogenic E. coli was most prevalent in 46.6%, thermophilic Campylobacter in 26.2% and Salmonellae was in 8.7% in that order. In the OK serotyping for 117 isolates of enteropathogenic E. coli, type 0141 : K85(20.5%), 0157:K88ac(14.5%), 0138:K81 and 0149:K91 (13.3%) were encountered most frequently. In the biotyping for 27 isolates of thermophilic Campylobacter, most strains of C. jejuni belonged to type I (50.0%) and II (25.0%), and most strains of C. coli belonged to biotype I (78.9%). In the serotyping for 9 strains of Salmonellae, 3 strains were grouped as D, 2 strains as C and 1 strain each was group B and E. The other 2 strains were untypable. The 117 isolates of enteropathogenic E. coli were resistant more than 90% to erythromycin, penicillin, tetracycline and streptomycin, whereas about 90% of the isolates were sensitive to kanamycin and gentamicin. In the case of Salmonellae, all of the isolates were resistant to penicillin, but about 89% of the isolates were sensitive to gentamicin and colistin. All of C. jejuni and C. coli isolates were resistant to cephalothin, but more than 89% of C. jejuni and C. coli were sensitive to kanamycin and gentamicin
Show more [+] Less [-]Enterotoxin production and plasmid detection of citrate utilizing Escherichia coli isolated from cattle
1988
Chae, T.C. | Choi, W.P. (Kyongbuk National Univ., Taegu (Korea R.). Coll. of Veterinary Medicine)
This paper deals with the 0 groups of citrate utilizing variants of Escherichia coli (Cit+ E. Coli) isolated from cattle, the production of colicin, hemolysin, K99 antigen, heat stable enterotoxin, and the isolation of plasmid DNA. Among 42 Cit + E. Coli, 12 strains were 020, 9 strains 08, 5 strains 045, 3 strains 0115, 1 strain 064, 1 strain 0139 and remaining strains (11) were untypable. Thirty-nine(81.3%)out of 48 Cit + E. Coli produced colicin and 13(27.0%) produced hemolysin. Of 12 cit + E. coli bearing K99 antigen, 6(50.0%) produced heat stable enterotoxin. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmids varied from 1 to 7 in 10 Cit+ E. Coli. Its molecular weight ranged from 2 to 50 Mdalton, and 50 Mdalton plasmid commonly existed in all strains
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