Water quality is critical for poultry farming. This study assessed the physical, chemical and microbiological quality of drinking water in small-layer farms in Southern Mozambique and identified potential risk factors for total coliform (TC) and Escherichia coli contamination of drinking water. In 20 farms, 57 samples were collected and examined for pH, nitrate content (NC), nitrite level (NL) and total hardness contents (TH). Furthermore, TC and E. coli growth were assessed at 37 °C. One hundred per cent of the drinking water was of acceptable quality in terms of pH (6.5-8.5), NC (50 mg/L) and NL (3 mg/L). Total hardness contents exceeded the recommended standard in 37.5% of borehole water samples and 91.7% of tap water samples, respectively. Total coliform and E. coli were found in 40% and 15% of water samples. Tap water samples had the greatest contamination, with TC and E. coli levels of 41.7% and 16.7%, respectively. Although not statistically significant, sampling from the beginning of the nipple line (p = 0.101, OR = 7.357, 95% confidence interval [CI]: 0.678-79.886) and not cleaning the rearing equipment regularly (p = 0.098, OR = 3.966, 95% CI: 0.766-20.280) were factors affecting the TC growth. Sampling from the tank water source (p = 0.001, OR = 0.005, 95% CI: 0.000-0.121) and borehole water source (OR = 13 585) and not cleaning the equipment consistently (p = 0.073, OR = 9.682, 95% CI: 0.810-115.68) were all factors affecting E. coli growth. It is concluded that the TH and microbiological quality of the drinking water of the study region are inadequate. Regular water quality assessments should be incorporated into Mozambican layer farm management to limit the potential for health concerns, and farmers should thoroughly clean and disinfect their rearing equipment. CONTRIBUTION: We should incorporate regular water quality assessments into Mozambican layer farm management to limit the potential for health concerns, and farmers should thoroughly clean and disinfect their rearing equipment

Escherichia coli is present in the normal intestinal flora but some strains can cause intestinal and extraintestinal diseases, and research on its presence in food of animal origin is in the interests of public health. This study was designed to characterise E. coli strains according to their origin, their carriage of virulence genes specific for certain pathogroups, and phylogenetic group affiliation.

Escherichia coli is a widespread environmental pathogen frequently causing dairy cow mastitis. This bacterium is particularly capable of acquiring antimicrobial resistance, which can have severe impacts on animal food safety and human health. The objective of the study was to investigate antimicrobial resistance and genetic correlations of E. coli from dairy cow mastitis cases in northern China.

Objective: The increase and prevalence of multidrug-resistant bacteria in livestock animals are serious public health concerns. This study aimed to identify the presence of the blaTEM gene in extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated from rectal swabs of apparently healthy pigs in Malang District, East Java, Indonesia. Materials and Methods: A total of 120 rectal swab samples were collected from the pigs. The rectal swabs were screened for the presence of E. coli using standard microbiological identifi¬cation procedures. The Kirby–Bauer disk diffusion method identified multidrug-resistant E. coli. Five different classes of antibiotics were used to identify multidrug-resistant isolates, including Ciprofloxacin, Trimethoprim, Tetracycline, Streptomycin, and Aztreonam. Multidrug-resistant E. coli isolates were characterized for the presence of ESBL using double-disk synergy test methods. The presence of blaTEM genes was determined using polymerase chain reaction methods. Results: The results of this study indicated that 107 (89.2%) out of 120 samples analyzed were positive for E. coli isolates. A total of 32 (29.9%) E. coli isolates were identified to be multidrug-re¬sistant and further subjected to molecular testing. The molecular analysis revealed (5; 15.6%) E. coli isolates to harbor the blaTEM gene. Conclusion: The results of this study revealed that pigs and products of pork origin must be considered a source of transmission of ESBL-producing E. coli to public health important under the food chain. [J Adv Vet Anim Res 2022; 9(3.000): 447-452]

This paper describes a fatal case of pneumonia in a 14-day-old dog caused by extraintestinal pathogenic Escherichia coli (ExPEC). The necropsy showed that almost all of left lobes of the lungs had dark-red consolidation. A histopathology examination revealed moderate acute fibrino-hemorrhagic necrotizing pneumonia with intralesional bacterial colonies. Non-suppurative epicarditis, congestion in the liver, and necrosis in the white pulp of the spleen also were found. E. coli with cytotoxic necrotizing factor 1 and α-hemolysin was isolated from the lung. This case was confirmed to have fatal pneumonia caused by ExPEC that led to a systemic infection.

Objective: Most people in Matrouh Governorate consume camel milk as a treatment for many diseases in a raw state to obtain nutritive value. Raw dromedary camel milk can be contaminated by Escherichia coli through fecal matter at any point of milk handling; therefore, it may lose its value and safety specifications. This survey aimed to estimate the incidence of E. coli in fresh camel milk. Materials and Methods: 100 fresh camel milk samples (50 from markets and 50 from farms) were randomly collected from different districts in Matrouh Governorate, Egypt, over 4 months for the detection of E. coli incidence through conventional bacterial isolation, molecular investigation, and gene sequencing. Results: The prevalence rates of E. coli in the examined market and farm raw camel milk based on conventional methods were 24% and 8%, respectively, while those by molecular identification using phoA as an E. coli determinate gene were 4% and 6%, respectively. Moreover, E. coli phoA gene phylogenetic analysis revealed high sequence similarity to E. coli strain CP033158.1 in India and E. coli strain CP047594.1 in China. Antibiotic sensitivity of E. coli isolates showed high suscep¬tibility to norfloxacin (10 μg) and cefoperazone (75 μg). On the other hand, high resistance was found in rifamycin (30 μg) and cefoxitin (30 μg). Conclusion: The results indicate that market camel milk is more contaminated than the farms' own. Additionally, antibiotic resistance is increasing due to antibiotic abuse. [J Adv Vet Anim Res 2022; 9(1.000): 138-143]