The study was conducted to determine the effect of the 5-Fluorouracil (5-FU) drug is a medication used to treat cancer or the knowledge of 5-FU drug(anticancer) effect might help to attribute these side on physiological and biochemical parameters in human by injection into a vein it is used for colon cancer, esophageal cancer, stomach cancer, pancreatic cancer, breast cancer and cervical cancer. 80 blood samples were obtained adult human divided into two groups for each group (n = 40), the first group (control) and the second group treatment with drug 5-fluorouracil. There are a significant decrease (P