Prevalence and management of ovulatory disturbances was recorded from 454 repeat breeding crossbred Jersey cattle of Kashmir valley during a 7 years period (2004-05 to 2010-11). Prevalence of anovulation and delayed ovulation was recorded 16.30 and 12.78% with an overall prevalence of 29.07% ovulatory disturbances. Anovulatory animals treated with RCG (Chorulon @ 1500 to 3000 IV i.v. per animal depending on body weight) showed conception rate (CR) of 86.36%; while those treated with Buserelin (Receptal @ 5ml Lv. per animal) as 100.00%, although this difference was statistically non-significant. Animals suffering from delayed ovulation inseminated twice, thrice or 4th times depending on the persistence of estrus signs showed overall CR of 87.93%.

Thirty Staphylococcus aureus isolates used in the study obtained from cattle (20) and goat (10) were haemolytic on blood agar. Twenty one of the isolates (14 from cattle, 7 from goats) produced a-haemolysis, 3 produced b-haemolysis (2 from cattle and 1 from goats), and 6 isolates (4 from cattle and 2 from goats) produced both a- and b-haemolysis. The haemolysins tested against erythrocytes from rabbit, cattle and horse in order to demonstrate a-, b- and d-toxins, respectively revealed that a- and b-toxins were produced by all the isolates but b toxin was produced by only 7 isolates from cattle and by 3 from goats. On titration it was recorded that highest titre was recorded for a-toxins (for cattle, 1:2560 and for goat, 1:1280) whereas the highest titres for b and d-toxins was similar (1:160) for cattle as well as goat isolates. The result obtained for qualitative and quantitative haemolysin assays correlated well with the haemolysis pattern seen on the blood agar plates.

An overall prevalence of 20.07% true anoestrus and delayed puberty in cattle was recorded out of 2202 gynaecological cases presented to the clinical complex and different clinical camps held at different locations of Kashmir valley over a period of three years (2005_2008). The highest prevalence of true anoestrus in cows was recorded in the 1st parity when they attained approximately 3 years of age and during autumn season.

Survivability of Listeria monocytogenes in clay loam soil was examined at 30° C for a period of 35 days. The microorganism inoculated in soil steadily declined from 3.5 × 10 6 g -1 to 3.9 × 10 4 g -1 (P = 0.05) on 35 th days. Destruction of normal soil microflora by heat treatment resulted in marginal rise in 3.3 × 10 6 g -1 of L. monocytogenes to 4.2 × 10 6 g -1 during the first 7 days, followed by steady decline to 5.6 × 10 4 g -1 on 35 th days. Heat treated soil inoculated with lower concentration of L. monocytogenes (3.6 × 10 4 g -1 ) supported sharp rise in its population to 4.8 × 10 6 g -1 (P=0.05) during the first weak which gradually declined, but remained higher (6.1 × 10 4 g -1 ) than the initial load even on 35 th days. In non-sterile soil, the rise was less appreciable but L. monocytogenes maintained its levels (10 4 g -1 ) during the experimental period. Survivability of the microorganisms was also observed in the soil with added chicken manure, cattle manure or sheep/goat manure.

Abortion, still birth, premature birth and mortality of cross bred dairy cattle (Jersey × Tharparkar/Red Sindhi) were noticed in the organized dairy farm of National Dairy Research Institute, Eastern Regional Station, Kalyani, Nadia, situated in hot and humid climatic area nearer to the river Ganges of West Bengal, India. The history of the farm revealed newly introduction of pure bred dairy cattle and outbreak of FMD during mid March to mid April, affected about 34% cross bred cows. During investigation, intermittent rise of temperature (104°F -108°F), anorexia, rapid respiration, progressive deterioration of health of animals and loss of milk production were also noticed. On the basis of past history, twenty suspected animals were taken for disease investigation. Repeated visit of the farm and repeated examinations of blood smears were done to observe any haemoprotozoan infections. Twenty to thirty percent of those suspected animals were found positive for Brucella antibodies by STAT, plate agglutination test and MRT. After a massive screening of blood smears, during the visit of third time, ultimately one animal (Identification number JT614) was found positive for the presence of Trypanosoma evansi infections in Giemsa stained blood smears. The infected and all suspected animals were successfully treated with single injection of a mixture of quinapyramine sulphate and chloride @ 7.4 mg/kg body weight subcutaneously. As a prophylactic measure, a mixture of quinapyramine sulphate and chloride @ 7.4 mg/kg body weight subcutaneously were also administered to all suspected animals prevented further occurrence of the disease in this dairy farm. It can be concluded that the iAfection with T. evansi in this farm has happened in a condition of intercurrent diseases with environmental stresses.

The present report was based on analysis of 410 cases (300 buffaloes and 110cattle) of reproductive disorders. Buffaloes (300 cases) constituted the major number of the total cases followed by cows (110 cases). Dystocia cases were the major obstetrical problem in buffaloes and cattle constituted 77 and 40 per cent, respectively.

Clinico-therapeutic studies were made on thirty clinical cases of lantana toxicity in cattle. The affected animals showed anorexia, constipation, icteric mucous membranes, lachrymation, oligurea,cracks on muzzle, dehydration and rumen stasis. Biochemical analysis revealed that 61.53 % of early presented cases and 100 % of late presented cases were having biphasic Vanden Bergh reaction. Hypoglycemia and increased total protein level were present in late presented cases. Markedly elevated values of total bilirubin, BUN, AST and alkaline phosphatase in both the early and late cases of lantana toxicity indicated hepatic and renal dysfunction.

The calf was a congenital abnormal stillbirth of Sahiwal breed of zebu cattle, with multiple musculoskeletal defects. It was born to a heifer in its first calving. The calving was normal; however, calf born had multiple anomalies. The body of the calf was flashy just like a rubber balloon filled with water (case of hydropsy). The body was without hairs (hypotrichosis). Skeleton was noncalcified and ribs were cartilaginous. The body was identifiable in three regions: head, thoraxabdomen, and limbs. Morphologically limbs were developed but were quite short in length with well-developed hoof. There was one eye like structure just above the mouth (case of cyclopia). The tongue was developed and was protruded from mouth. There was one additional structure on the head, looked like outgrowth of muscles covered with thin skin, had openings of nostrils on the end. In autopsy abdominal body cavity was found filled with fluid. The visceral organ seemed normal. It was identified as female; however, ovaries and genital tract could not be traced and examined. It was the first calving of its dam with the complete gestation period of 287 days like a normal period in cattle. Pieces of tissue from lung and blood from the heart and vena cava were collected; however, cultures were found heavily contaminated with bacterial growth. The actual cause of the defects could not be established, might be defects of certain genes responsible for incomplete growth and development.