Refine search
Results 1-5 of 5
Characterization of Akabane virus (KV0505) from cattle in Korea
2008
Yang, D.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: yangdk@nvrqs.go.kr | Kim, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kweon, C.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, S.H. (Jeju Veterinary Research Institute, Jeju, Republic of Korea)
Akabane disease is caused by an arthropod-borne viral pathogen and leads congenital abnormalities of the central nervous system in infected ruminants. One isolate, KV0505, showed cytopathic effect in Vero cells. The KV0505 isolate was obtained from plasma, which was collected from a cattle raised on Jeju Island in May 2005. Jeju Island is located near the southern part of the Korean peninsula. The isolate was confirmed as Akabane virus (AKAV) by immunofluorescence assay using AKAV specific monoclonal antibodies and reverse transcription polymerase chain reaction (RT-PCR). Suckling mice inoculated with the isolate showed signs of paralysis and died within 10 days post-inoculation. Comparisons of the KV0505 N gene sequence with 39 other known AKAV strains revealed nucleotide homologies ranging from 83.6% (MP496 strain) to 99.7% (M171 strain). When compared with the K-9 strain, which was isolated from a cow in Korea in 1994, the nucleotide sequence homology with the N gene was 99.7%. Thus, genes of the KV0505 isolate were closely related to those of the M171 strain, which were clustered into the Ic group of AKAV.
Show more [+] Less [-]Electrocution caused by a fallen electric wire in Korean native cattles
2008
Bae, Y.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: baeyc@nvrqs.go.kr | Lee, K.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Heo, J.H. (Gyeongnam Livestock Promotion Institute South-branch, Tongyoung, Republic of Korea) | Lee, O.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
We report the electrocution of Korean native cattle by a fallen electric wire, which caused the death of thirteen animals. The owner of the cattle felt an electric shock on touching the steel pen and found a fallen 220-V wire on the roof of the barn; additionally, the roof was singed. Clinically, the animals developed spasm, difficulty breathing, and excessive salivation. Histopathologically, many visceral organs revealed severe congestion or hemorrhage, which is consistent with previous reports. This study revealed that the proper installation of electric wires on farms is essential to prevent economic loss by electrocution.
Show more [+] Less [-]Idiopathic eosinophilic myositis in Korean native cattle (Bos taurus coreanae)
2008
Rhee, S.H. (Chonbuk National University, Jeonju, Republic of Korea) | Yu, I.J. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, J.H. (Chonbuk National University, Jeonju, Republic of Korea) | Kwon, J.K. (Chonbuk National University, Jeonju, Republic of Korea) | Park, J.H. (Chonbuk National University, Jeonju, Republic of Korea) | You, M.J. (Chonbuk National University, Jeonju, Republic of Korea) | Lee, J.W. (Jeongeup Branch, Institute of Livestock and Veterinary Research, Jeongeup, Republic of Korea) | Park, H.J. (Chonbuk National University, Jeonju, Republic of Korea) | Chekarova, Irina (Chonbuk National University, Jeonju, Republic of Korea) | Camer, Gerry Amor (University of Eastern Philippines, Catarman, Northern Samar, Philippines) | Lim, C.W. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, B.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: bskims@chonbuk.ac.kr
Eosinophilic myositis lesions are characterized by severe eosinophil infiltration along muscles of affected animals. The exact cause of the lesion remains controversial and the carcass is condemned once this lesion is seen during meat inspection. A cow slaughtered in Chonbuk province, Korea was observed to have disseminated pale foci throughout the musculature; meat samples were obtained and macroscopically investigated. Cut ends of neck and thigh muscle tissues showed variably sized, multifocal pale white-grayish nodular lesions. Histopathological examination consistently revealed inflammatory lesions with adjacent infiltration of eosinophilic granulocytes and focal necrotic calcification. However, no parasites, including Sarcocystis sp., could be discerned in the affected carcass. This case was diagnosed as idiopathic eosinophilic myositis in cattle.
Show more [+] Less [-]Effects of anti-tick cocktail vaccine against Rhipicephalus appendiculatus
2008
Imamura, S.(Hokkaido Univ., Sapporo (Japan)) | Konnai, S. | da Silva Vaz, I.Jr. | Yamada, S. | Nakajima, C. | Ito, Y. | Tajima, T. | Yasuda, J. | Simuunza, M. | Onuma, M. | Ohashi, K.
Rhipicephalus appendiculatus serpin-3 (RAS-3), R. appendiculatus serpin-4 (RAS-4) and a 36kDa immuno-dominant protein of R. appendiculatus (RIM36) were reported as candidate antigens for the anti-tick vaccine to control ixodid ticks. In the present study, we generated recombinant proteins of RAS-3 (rRAS-3), RAS-4 (rRAS-4) and RIM36 (rRIM36), and assessed their potency as an anti-tick cocktail vaccine in cattle model. RT-PCR analysis showed that RAS-3, RAS-4 and RIM136 transcripts were detected in both adult male and female ticks during feeding. Immunization of cattle with the combination of rRAS-3, rRAS-4 and rRIM36 had raised antibodies against all recombinants and anti-sera had reacted with the molecules from the tick salivary gland extract. Tick infestation challenge demonstrated protective immunity against female ticks, resulting in mortality rates of 39.5 and 12.8 % for the vaccinated and control groups, respectively. Moreover, the mortality rate of Theileria parva-infected female ticks was 48.5 and 10.8 % in the vaccinated and control group, respectively. In order to evaluate the levels of pathogen transmission capacity by T. parva-infected ticks fed on immunized cattle, the occurrence of T. parva in the bovine parotid lymph node and peripheral blood was also determined and quantified by real-time PCR. Although the infection with T. parva could not be protected by the vaccine, the occurrence of pathogen in peripheral blood was delayed 1 to 2 days after the infestation challenge in vaccinated group. These results suggest that this cocktail vaccine plays a role in the prevention of tick infestation.
Show more [+] Less [-]Availability of oral swab sample for the detection of bovine viral diarrhea virus (BVDV) gene from the cattle persistently infected with BVDV
2008
Tajima, M.(Hokkaido Univ., Sapporo (Japan)) | Ohsaki, T. | Okazawa, M. | Yasutomi, I.
Bovine nasal and oral discharges were used as samples for bovine viral diarrhea virus (BVDV) gene detection. Viral genes in serum (S), nasal discharge (N) and oral discharge (O) were quantified with real-time polymerase chain reaction using SYBR Green by the relative quantification method, and findings were compared among samples. Although the quantity of the BVDV gene in S was greater than those in N and O, all samples were available to identify persistently infected (PI) cattle with BVDV by reverse transcription polymerase chain reaction (RT-PCR). The swab samples were able to be stored for a few days at 4degC with a little decrease of amplification signal in RT-PCR. Oral swab sampling was easier than nasal swab sampling, and was also less uncomfortable for the cattle than other sampling methods without pain or unnecessary retention. This sampling method can be performed without any special technique and equipment. Therefore, the oral swab sampling method is useful for screening to detect BVDV PI cattle by RT-PCR.
Show more [+] Less [-]