Thirty Staphylococcus aureus isolates used in the study obtained from cattle (20) and goat (10) were haemolytic on blood agar. Twenty one of the isolates (14 from cattle, 7 from goats) produced a-haemolysis, 3 produced b-haemolysis (2 from cattle and 1 from goats), and 6 isolates (4 from cattle and 2 from goats) produced both a- and b-haemolysis. The haemolysins tested against erythrocytes from rabbit, cattle and horse in order to demonstrate a-, b- and d-toxins, respectively revealed that a- and b-toxins were produced by all the isolates but b toxin was produced by only 7 isolates from cattle and by 3 from goats. On titration it was recorded that highest titre was recorded for a-toxins (for cattle, 1:2560 and for goat, 1:1280) whereas the highest titres for b and d-toxins was similar (1:160) for cattle as well as goat isolates. The result obtained for qualitative and quantitative haemolysin assays correlated well with the haemolysis pattern seen on the blood agar plates.

The calf was a congenital abnormal stillbirth of Sahiwal breed of zebu cattle, with multiple musculoskeletal defects. It was born to a heifer in its first calving. The calving was normal; however, calf born had multiple anomalies. The body of the calf was flashy just like a rubber balloon filled with water (case of hydropsy). The body was without hairs (hypotrichosis). Skeleton was noncalcified and ribs were cartilaginous. The body was identifiable in three regions: head, thoraxabdomen, and limbs. Morphologically limbs were developed but were quite short in length with well-developed hoof. There was one eye like structure just above the mouth (case of cyclopia). The tongue was developed and was protruded from mouth. There was one additional structure on the head, looked like outgrowth of muscles covered with thin skin, had openings of nostrils on the end. In autopsy abdominal body cavity was found filled with fluid. The visceral organ seemed normal. It was identified as female; however, ovaries and genital tract could not be traced and examined. It was the first calving of its dam with the complete gestation period of 287 days like a normal period in cattle. Pieces of tissue from lung and blood from the heart and vena cava were collected; however, cultures were found heavily contaminated with bacterial growth. The actual cause of the defects could not be established, might be defects of certain genes responsible for incomplete growth and development.