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Haemolytic properties and titration of haemolysins of Staphylococcus aureus of milk origin from cattle and goat with clinical mastitis.
2010
Upadhyay, A. | Kataria, A.K.
Thirty Staphylococcus aureus isolates used in the study obtained from cattle (20) and goat (10) were haemolytic on blood agar. Twenty one of the isolates (14 from cattle, 7 from goats) produced a-haemolysis, 3 produced b-haemolysis (2 from cattle and 1 from goats), and 6 isolates (4 from cattle and 2 from goats) produced both a- and b-haemolysis. The haemolysins tested against erythrocytes from rabbit, cattle and horse in order to demonstrate a-, b- and d-toxins, respectively revealed that a- and b-toxins were produced by all the isolates but b toxin was produced by only 7 isolates from cattle and by 3 from goats. On titration it was recorded that highest titre was recorded for a-toxins (for cattle, 1:2560 and for goat, 1:1280) whereas the highest titres for b and d-toxins was similar (1:160) for cattle as well as goat isolates. The result obtained for qualitative and quantitative haemolysin assays correlated well with the haemolysis pattern seen on the blood agar plates.
Show more [+] Less [-]Freedom status of dourine (Trypanosoma equiperdum) in Malaysia
2013
Chandrawathani P. | Uppal P. K. | Norazura A. H. | Chin, S. W. | Premaalatha B. | Ramlan M.
The objective of this study was to determine the seroprevalence of dourine from imported horses using Complement Fixation Test. Dourine is classified as a OIE listed B disease (OIE Terrestrial Manual 2008) where equines are mainly susceptible to it and it had occurred in many countries such as Africa, the Middle East, South America and South-eastern Europe. Malaysia regularly imports Australian horses for the equestrian sector which encompasses activities such as racing and endurance as well as other recreational or leisure activities in Malaysia. As such, awareness towards this disease is important during importation of horses to avoid dourine embark into Malaysia. A total of 288 horse sera samples obtained from animal quarantine stations and private stables were examined for dourine by complement fixation test. Results showed serologically negative results for dourine with all the samples tested.
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