The first outbreak of aujeszky's disease (AD) was identified from piggery located at the southern part of Korea in July, 1987. This piggery suffered from a significant economic loss caused by unexpected piglet mortality and reproductive failure. Etiologic viral agents were isolated from tonsil and spleen of the infected piglets, and the isolates produced a typical cytopathic effect of herpesvirus with giant cell formation when inoculated in many different cells. Subsequently the field isolates were characterized as suid herpesvirus I by cross-neutralization test and indirect fluorescence assay utilizing specific monoclonal antibody, and proved to be a pathogenic strain of AD virus(ADV).

Introduction: Porcine pleuropneumonia inflicts important economic losses on most commercial herds. Detection of subclinical or chronic infection in animals still remains a challenge, as isolation and identification of A. pleuropneumoniae serotypes is difficult and quantification of the bacteria on agar plates is often almost impossible. The aim of the study was to develop and evaluate a serotype-specific quantitative TaqMan probe-based PCR for detection of serotype 2 in pig lungs, tonsils, and nasal swabs.Material and Methods: The primers were designed from the capsular polysaccharide biosynthesis genes of A. pleuropneumoniae serotype 2. PCR specificity and sensitivity were evaluated using reference strains and several other bacterial species commonly isolated from pigs.Results: The real-time qPCR for detection of A. pleuropneumoniae serotype 2 was highly specific and gave no false positives with other serotypes or different bacterial species of pig origin. The detection limit for pure culture was 1.2 × 10⁴ CFU/mL, for lung tissue and nasal swabs it was 1.2 × 10⁵ CFU/mL, and for tonsils - 1.2 × 10⁵ CFU/mL.Conclusion: The method can be used to serotype A. pleuropneumoniae isolates obtained during cultivation and to detect and identify A. pleuropneumoniae serotype 2 directly in nasal swabs and tonsil scrapings obtained from live pigs or lung tissue and tonsils collected post-mortem.

Introduction: The study objective was to develop and evaluate a new TaqMan multiplex real-time PCR method for Salmonella, L. monocytogenes, and verotoxigenic Escherichia coli (VTEC) detection in slaughtered animal carcasses.

Introduction: The study objective was to develop and evaluate a new TaqMan multiplex real-time PCR method for Salmonella, L. monocytogenes, and verotoxigenic Escherichia coli (VTEC) detection in slaughtered animal carcasses.Material and Methods: The procedure included an enrichment step, DNA extraction, and two multiplex real-time PCRs. The first PCR detected the invA and hly genes of Salmonella and L. monocytogenes respectively, the second the vtx1, vtx2, and eae genes of VTEC.Results: The validation of this method resulted in 100% relative sensitivity, specificity, and accuracy as compared to the reference ISO methods. The limit of detection per swab sample was established at 1 cfu for Salmonella and L. monocytogenes and 2 cfu for VTEC. The authors analysed 265 slaughterhouse-collected swabs from cattle, pig, and poultry carcasses. Among 125 from cattle, 51 were positive for VTEC, 29 for Salmonella, and 1 for L. monocytogenes. Among swabs from pig carcasses (n = 95), three, two, and one sample were positive for these pathogens respectively. None of the microorganisms tested for was identified in 45 samples of poultry origin.Conclusion: The obtained results showed that the method developed can rapidly identify the main bacterial pathogens that may contaminate carcasses of food-producing animals.

Introduction: Porcine pleuropneumonia inflicts important economic losses on most commercial herds. Detection of subclinical or chronic infection in animals still remains a challenge, as isolation and identification of A. pleuropneumoniae serotypes is difficult and quantification of the bacteria on agar plates is often almost impossible. The aim of the study was to develop and evaluate a serotype-specific quantitative TaqMan probe-based PCR for detection of serotype 2 in pig lungs, tonsils, and nasal swabs.

In this study, within the boundaries of the river Kizilirmak River Basin, Kirikkale and Ankara aimed at the determination of the fauna of Simuliidae.           The materials of the study were a total of 8877 samples including 7509 larva, 372 end-stage larva and 996 pupae from nine different station (Kalecik, Bala, Yahşihan, Merkez (Kirikkale), Bahşılı, Keskin, Karakeçili, Çelebi, Sulakyurt) of Kızılırmak river and branches of Kızılırmak Basin bounded by the borders of Kırıkkale and Ankara province between March 2009 and October 2010. Diagnostic examined and assessed by key stereo microscope. After the idenfications, S. petricolum, S. equinum, S. pseudequinum, S. lineatum, S. balcanicum, S. erythrocephalum and S. alajense were detected as species level, S.angustipes and S. ornatum were detected as group level. Simulium. petricolum (25.73%) was the most prevalent specie while S. alajense (0.02%) was the least. In conclusion, Simuliiumlineatum, S. pseudequinum, S. balcanicum and S. alajense were detected in Kızılırmak basin previously but S. petricolum,S. equinum and S. erythrocephalum were recorded first time in this place. Keywords: Ankara, identification, Kirikkale, Kızılırmak River Basin, prevalence, Simulium spp.

Salmonella is gram negative, facultative anaerobe and zoonotic bacteria and their serotyping based on the Kauffmann-White schema according to these three different antigens including somatic (O) and flagellar (H) antigens. Among these serotypes, S. enterica subsp. enterica (I) is the most important factor responsible for Salmonella infections. It has been reported in several publications that Salmonella infections caused by S. Rubislaw have started to be seen in recent years with the increase of exotic animal breeding for hobby purposes. In the serotyping of Salmonella spp. positive environmental isolate sample, which was sent from a poultry establishment to the Bacteriological Diagnostic Laboratory of the Central Research Institute of Veterinary Control on 20.10.2019, Salmonella enterica serotype Rubislaw was identified. This isolate is the first specimen in which S. Rubislaw was identified. It is highly probable that similar cases in which exotic animals play an important role in transmission are more common in our country in the near future.

In this study, three different methods were compared for the identification of some Gram-positive and Gramnegative reference bacteria. Material and Methods: For this purpose, the identification accuracy rates of Enterococcus faecalis, Enterococcus faecium, Streptococcus agalactiae, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Serratia marcescens were analysed by conventional bacteriological methods, commercial bacterial identification test kit (Microgen™ ID) and automated bacteria identification system (BD Phoenix 100™).