The study was focused to investigate the occurrence of a fatal capture myopathy (CM) after chemical immobilization during translocation in different places and to evaluate serum enzymes of stressed deer and pathologic alteration of vital organs of dead animals due to CM. Materials and Methods: The experimental data was collected from Bangladesh National Zoo, Dhaka and the experiment was conducted at the Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh. Immediate after capture the animals were allowed to normalize body temperature by pouring sufficient water over the body. Peripheral blood was aspirated from jugular vein for serum biochemical analysis. Once the animals died, vital organs were collected and processed for histology.Data from 2013 to 2018 revealed 178 animals captured through darting and among them 40 animals died due to post-capture myopathy reflecting death rate of 22.47%. We have closely studied on 16 animals captured on different occasions. Serum enzyme analysis were exhibited increased levels of ALT, AST, Bilirubin, Creatinine, BUN, LDH, CK, Troponin, Cholesterol, Triglyceride, HDL and LDL and were highly indicative of stress-linked muscle and organ damage. The macroscopic lesions consisted of muscular and cardiac degeneration, edema, hemorrhage and congestion in lung, adrenal gland and in kidney. Microscopically there were loss of striation and fragmentation of skeletal muscle, formation of contraction band necrosis in myocardial fiber, degenerative changes in renal tubule and formation of central intraluminal eosinophilic casts.The pathological findings were indicative of capture myopathy in spotted deer. This report underlines that mortality from capture is a risk that must be considered during restocking programs.

Hexosamine concentration (an index of proteoglycan content), DNA content (an index of cellularity), and [35S]sulfate incorporation (an index of proteoglycan synthesis) of articular cartilage were measured in biopsy specimens from medial proximal sesamoid bone, medial condyle of the third metacarpal bone, and proximal dorsal rim of the proximal phalanx in both metacarpophalangeal joints of 6 adult horses. One limb was then placed in a fiberglass cast that extended down from the proximal portion of the metacarpus and enclosed the hoof; the other limb was not casted. After 30 days of staff confinement, additional specimens were taken from the medial proximal sesamoid bone, medial condyle of the third metacarpal bone, midproximal portion of the proximal phalanx, distal portion of the proximal phalanx, and proximal portion of the middle phalanx of both limbs for comparison. Immobilization resulted in an apparent decrease in the hexosamine content of the cartilage when the 30-day immobilized vs 30-day mobilized specimens were analyzed. This decrease was accentuated by opposing trends in the 2 limbs. The immobilized cartilage tended to lose hexosamine, whereas the mobilized limb tended to gain hexosamine during the 30-day period; a similar trend also was seen with [31S] incorporation, but this trend was not statistically significant. The largest change was a significant increase in glycosaminoglycan synthesis in the mobilized limb, compared with little change in the immobilized joint cartilage. We concluded that contralateral limbs are unsuitable for controls in immobilization studies because of their biological response to increased weight bearing. We also concluded that the changes in articular cartilage found following simple cast immobilization of 30 days' duration are minor and probably of little clinical consequence.

Interleukin (IL)-6, a cytokine for host defense responses to infection and imflammation, is known to be induced by non-invasive physical or psychological stress, too. To test possible involvement of brain IL-1 in the stress-induced IL-6 production, IL-1 mRNA expression in the hypothalamus, in parallel with blood IL-6 Ievel, was examined in rats subjected to restriction of their movement (immobilization stress). When rats were immobilized, the hypothalamic IL-1 beta mRNA Ievel was increased in 1 hr, followed by progressive rises in the serum IL-6 level. The immobilization-induced rise in serum IL-6 was mimicked by intracerebroventricular (icv) administration of IL-1 beta under normal conditions, whereas it was attenuated by icv injection of an IL-1 receptor antagonist. These results indicate that IL-1 in the hypothalamus plays a pivotal mediating role in the stress-induced peripheral IL-6 production.