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Baypamun® action in hamsters experimentally infected with Leptospira interrogans serogroup Canicola | Ação do Baypamun® em hamsters experimentalmente infectados com Leptospira interrogans, sorogrupo canicola
2001
Hélio Langoni | Rui Seabra Júnior | Kenio Gouveia Cabral | Eva Laurice Pereira Cunha
Baypamun® is an immunity modulator recommended as prophylactic and therapeutic use. In the present study, this product was used in the hamsters experimentally infected with Leptospira interrogans serogroup canicola. The animals were divided in 5 groups, with 20 animals each. This product was used as a therapeutic, prophylactic and as adjuvant. As a therapeutic use all the animals died. Despite of eight (40.0%) survivors, the prophylactic use wasn't also totally favorable due to leptospire recovering from kidneys of those animals. Based on the analysis of antibodies levels among animals that received vaccine and vaccine with Baypamun® as adjuvante, this product didn't conferred superior humoral answer. However, other studies should be accomplished utilizing this product in a longer period as well as verify the cellular answer by Baypamun® use as adjuvant in the infectious disease. | Baypamun® é um imunomodulador recomendado na profilaxia e terapêutica de enfermidades. No presente estudo, este produto foi usado em hamsters experimentalmente infectados com Leptospira interrogans, sorogrupo canicola. Estes animais foram divididos em 5 grupos com 20 animais cada. O produto foi usado como terapêutico, profilático e como adjuvante. Quando usado de forma terapêutica, todos os animais vieram a óbito. Quando usado na profilaxia, 8 animais (40%) sobreviveram e foi possível recuperar o agente a partir dos rins desses animais. Baseado na análise do título de anticorpos nos animais que receberam ou não o Baypamun como adjuvante vacinal, o produto não elevou a resposta humoral. Os resultados sugerem, entretanto, outros estudos que utilizem o produto por períodos mais prolongados, além de se verificar a resposta celular para se avaliar o seu efeito como adjuvante nas infecções.
Show more [+] Less [-]Immunity ticks Rhipicephalus sanguineus (Latreille) in crab-eating-fox Cerdocyon thous (Linnaeus) and mongrel dogs | Imunidade a carrapatos Rhipicephalus sanguineus (Acarina: Ixodidae) em cachorro-do-mato Cerdocyon thous (Linnaeus) e no cão doméstico
1995
Beatrix Rossetti Ferreira | Gervásio Henrique Bechara
Many parasite-host relationships are characterized by the development of resistance by the host, thus limiting the number of parasites. However, in the domestic dog X <em>Rhipicephalus sanguineus<strong> </strong></em>(brown dog tick) relationship, this does not occur. The hypothesis that the domestic dog has lost this faculty, following the process of its domestication, was investigated by comparing the mongrel dog’s and the crab-eating-fox <em>Cerdocyon thous's<strong> </strong></em>capacity of acquiring immunity to <em>R. sanguineus<strong> </strong></em>ticks in two situations: after, 3 successive infestations with adult ticks, and immunisation with an unfed whole tick <em>R. sanguineus </em>extract, followed by a challenge infestation with the same tick species. Evaluation of resistance acquisition, in both experimental conditions, was based on nutricional and reproductive performaces of the female ticks during and after infestations. The results showed a small, significant difference between the domestic dog and the crab-eating-fox. Nevertheless, these differences were not considered relevant enough to characterize an effective immunity. | Algumas das relações hospedeiro-parasita são marcadas por desenvolvimento de resistência pelo hospedeiro, limitando o número de parasitas. Tal não ocorre na relação cão doméstico x <em>Rhipicephalus sanguineus</em> (carrapato do cão). Teria o cão doméstico perdido aquela capacidade em seu processo de domesticação? Esta hipótese foi testada em duas condições, comparando-se a aquisição de imunidade ao carrapato <em>R. sanguineus</em> pelo cão doméstico em relação ao cachorro-do-mato <em>Cerdocyon thous</em> após três infestações sucessivas por carrapatos adultos, ou através de infestação desafio após imunização com um homogenato bruto de carrapatos da mesma espécie. A aquisição ou não de resistência foi avaliada a partir da análise do desempenho alimentar e reprodutivo das carrapatas durante e após as infestações. Os resultados indicaram uma pequena diferença significativa entre o cão doméstico e o cachorro-do-mato quanto ao desenvolvimento de resistência ao carrapato <em>R. sanguineus</em>, que, entretanto, não foi considerada relevante na caracterização de uma imunidade efetiva.
Show more [+] Less [-]Falha da transferência de imunidade passiva em cordeiros mestiços (Santa Inês x Dorper) e estudo do proteinograma do nascimento até o desmame | Failure of passive immunity transfer in crossbred lambs (Santa Inês x Dorper) and proteinogram study from birth until waned
2013
Joselito Nunes Costa | Débora de Fátima Matias da Silva | Carla Caroline Valença de Lima | Thiago Sampaio de Souza | Alexandre Lôpo Araújo | Antônio Oliveira Costa Neto | Maria Angela Ornelas de Almeida
<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings> </xml><![endif]--><p class="MsoNormal">Analisou-se a ocorrência da Falha de Transferência da Imunidade Passiva (FTIP) em 45 cordeiros nascidos de ovelhas saudáveis e pluríparas. Estes animais foram acompanhados do nascimento até o desmame (90 dias), por meio de avaliações físicas, do hemograma, determinação de proteínas séricas totais e seu fracionamento eletroforético entre 24-72h pós-nascimento (p.n.), aos 7, 15, 30, 60 e 90 dias de idade (quando era realizada a desmama). Adotando-se o ponto de corte para proteínas séricas totais da ordem de 4,5g/dL, obteve-se a ocorrência de FTIP de 24,4% (11 animais). Os cordeiros com FTIP apresentaram menores valores (p<0,05) de proteínas séricas, albumina, alfaglobulina, betaglobulina 1 e gamaglobulinas entre 24-72h p.n. quando comparados aos dos 34 animais que não tiveram FTIP. 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mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} </style> <![endif]--> | <!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings> </xml><![endif]--><p class="MsoNormal"><span style="mso-ansi-language: EN-US;" lang="EN-US">It was analyzed the occurrence of the failure of passive transfer (FTIP) of the immunity 45 born lambs of healthy and pluriparous ewes. These animals were accompanied from the birth to the wean (90 days), when clinical evaluations were accomplished, hemogram, determination of total serum proteins and their electrophoretic fractions, 24-72h after the birth, to the 7, 15, 30, 60 and 90 days of age (at the same time of the wean). Adopting the cut point for total serum proteins of the order of 4.5g/dL, it was obtained the occurrence of FTIP of 24.4% (11 animals). The lambs with FTIP presented lowers values (p<0.05) of serum proteins, albumin, alpha-globulin, beta-globulin 1 and gamma globulins when compared to the 34 animals that have no FTIP. However, from 7th days of life it was not observed significant differences of these variables between groups, indicating the occurrence of a process of active production of proteins by group FTIP.</span></p>
Show more [+] Less [-]A pro-inflammatory effect of foot and mouth disease vírus on immune and non immune guinea pigs | Efeito pró-inflamatório do vírus da febre aftosa em cobaias imunes e não imunes
1992
Helio José Montassier | Gervásio Henrique Bechara | Euclides Braga Malheiros
The O<sub>1Campos </sub>strain of foot and mouth disease virus (FMDV) used as inducing agent in the pleurisy model was able to trigger a pro-inflammatory effect on normal and immune guinea pigs. The proinflammatory activity which was detected at two times of the pleurisy (24 and 48 hours) on normal guinea pigs was characterized only by mononuclear (MN) cell influx, during the first interval of the reaction and by edematogenic effect, MN and polimorphonuclcar (PMN) leucocyte migration, at the last time of the reaction. The inflammatory reaction profiles recorded on immune guinea pigs (vaccinated with anti-O<sub>1Campos </sub> oil adjuvanted vaccine), both after 7 and 30 days post vaccination (pv) have showed, in both interval, lower intensities than that observed in normal guinea pigs, although in the 7 days PV guinea pigs the accumulations of total leucocytes and PMN were similar to that displayed by normal animals, after 48 hours of the reaction. Besides, on thirty days PV guinea pigs the FMDV induced a significant increase in volume of exudate and MN cell infiltration, after 24 hours, and all of the inflammatory parameters values dropped to normal levels, during the second interval of the reaction. It was found a negative association between the increase in serum neutralizing antibody titer, from 7 to 30 days PV and the intensities of pleural inflammatory parameters on the immune guinea pigs. The pleurisy test revealed itself feasible to evaluate the pro-inflammatory activity of FMDV. | <p>A estirpe O<sub>1Campos</sub> do vírus da febre aftosa (VFA) usada como agente indutor do teste de pleurisia foi capaz de desencadear um efeito pró-inflamatório em cobaias normais e imunes. A atividade pró-inflamatória do VFA, detectada em dois intervalos de pleurisia (24 e 48 horas) foi demonstrada, somente por quimiotaxia de leucócitos mononucleares (MN), no primeiro intervalo e por efeito edematogênico, migração de MN e polimorfonucleares (PMN), no último intervalo de reação. Os perfis de reação inflamatória induzida pelo VFA em cobaias imunes (imunizadas com vacinas oleosas anti-VFAO<sub>1Campos</sub>), aos 7 e aos 30 dias pós-vacinação (PV) apresentaram imensidades mais baixas do que as observadas em cobaias normais, embora nas cobaias com 7 dias de vacinação a quimiotaxia de leucócitos totais e de PMN tenha sido similar àquela encontrada nos animais normais, no intervalo de 48 horas de reação. Ademais, nas cobaias com 30 dias PV, o VFA induziu um aumento significante no volume de exsudato e na infiltração de MN, no intervalo de 24 horas, sendo que os valores de todos os parâmetros do exsudato inflamatório caíram a níveis normais, no segundo intervalo de reação. Nas cobaias imunes foi observada uma associação negativa entre o aumento no título de anticorpos soro-neutralizantes, de 7 para 30 dias PV e as intensidades dos parâmetros inflamatórios pleurais. O teste de pleurisia revelou-se um procedimento adequado para avaliar a atividade próinflamatória do VFA.</p>
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