Investigation of immunity of broiler chickens of Kobb-500 cross vaccinated by national associated viral vaccine and foreign (ABIC, Israel; Vitex XXI vek Seva) vaccines against Newcastle disease and infectious bronchitis was investigated in the conditions of the Republic of Belarus. In course of studies there was analyzed the content of immunoglobulin in blood serum, as well as the influence of living vaccines on immunity formation. Research results showed that broiler chickens immunization against Newcastle disease and infectious bronchitis by national and foreign vaccine produced by ABIC company made it possible to increase the phagocytic activity of pseudoeosinophils, bactericidalal and lysozyme activity of blood serum, that proved the activation of nonspecific protection factors. At the same time in blood serum there was stated the increasing of immunoglobulin number in 1,5-3,0 times in comparison with control groups. Economic efficiency of all analyzed vaccines was analyzed

In the conditions of the Republic of Belarus there was developed and a simple and applicable for industrial production method of immunoglobulin production against animal salmonellosis. The following initial raw materials were used for production of the specific immunoglobulin: blood serum of bulls which were hyperimmunized with formolated antigen on the basis of four serovars: S. typimurium; S. abortusovis; S. dublin; S. choleraesuis. In course of the study there were analyzed chemical and biological indexes of immunoglobulin of experimemtal series; determination of the preparation doses for white mice and pigeons. The experimental work on production of immunoglobulin against salmonellosis made it possible to develop a technological scheme of the analyzed preparation production. In course of the experiment there were found optimal conditions for serum fractionation; there were developed methods of its conservation, sterilization and quality control of the specific immunoglobulin.

Determination of the positive aspects of application of a feed additive on the basis of sodium humate and malt sprouts fortified with oaky extract in diets of high-productive cows in dry period was realized in the conditions of the Republic of Belarus. Cows of black-and-white breed with average body weight 600 kg and milk-yield 6000-7000 kg were used in the experiment. Cows of experimental groups in addition to their main diet were fed with the resolved additive in doses 10-20 ml/100kg of live weight. Formula of humic additive with draft name Ehkolin-3 was developed on the basis of Ehkolin additive. Ehkolin additive in its composition contained the following compounds: organic substances; mineral substances; humic substances; melanoids; reducing agents; phenolic compounds; organic acids, and total nitrogen. Research results showed that application of the analyzed additive promoted the increasing enzyme activity in blood serum. Body weight of new born calves in experimental groups was higher than in control group. Symptoms of diseases of the digestive tract disappeared in 4-5 days in the experimental calves. Cows of the experimental groups had good colostral milk with density 1056 kg/mE3 and 55% of immunoglobulins. Impregnation capacity of in one experimental group was 50% after the first insemination, and only 33,3% and 20% in the control and the second experimental group, respectively

Analysis of manifestation of chlamydial infection of cattle in the conditions of forage affect with mycotoxins was realized in the conditions of the Republic of Belarus. Research results showed that chlamydial infection proceeded in association with aflatoxin, T-2 toxin, deoxynivalenol, zearalenone and ochratoxin. In course of the biochemical analysis there was noted the lowering of carotene on 23%, and vitamin A – in two times; also there was slight lowering of alkaline reserve. The obtained data proved the substantial changes in content of sub-populations of lymphocytes in cow blood in dry and postnatal period. During the immunological study of immunoglobulins in blood serum of cows sick with postpartum chlamydial endometritis it was revealed that titre of immunoglobulin A was on 31% higher. Level of immunoglobulins G and M differed slightl

Evaluation of a quantity and phagocyte activity of leucocytes, immunoglobulins, T- and B-lymphocytes in blood serum of sheep in the process of stimulation of central nervous system with caffeine sodiobenzoate for the increasing of nonspecific animal resistance was realized in the conditions of the Republic of Belarus. Romanov yeld ewes were divided into two groups. Experimental sheep were entered caffeine sodiobenzoate intramuscularly in dose 0,012 g/kg of live weight twice a day with 6 hours interval. Controlled group of sheep were entered physiological salt solution in accordance with the same scheme. Blood samples were taken before preparation entering and after 1, 3, 6, 12 and 24 hours of test beginning. Immunoglobulin concentration in blood serum was estimated by electrophoresis method in polyacrylamide gel. Quantity of T- and B- lymphocytes was estimated by active rosette test. Phagocyte activity of leucocytes was estimated with application of germ culture St. albus. Leucocytes number was accounted in Gorjaev's chamber. Research results showed that under conditions of central nervous system stimulation by caffeine sodiobenzoate there was stated the increasing of nonspecific resistance. It was proved by the increasing of the total number and phagocytic activity of leukocytes, immunoglobulins (IgG, IgA and IgM), T- and B- lymphocytes

The data about influence of the developed home-produced inactivated vaccine against pasteurellosis of fur animals on blood, biochemical, immunity indicators at mink are cited. It is established, that application of the given preparation conducts to formation of the necessary immune answer. The developed domestic inactivated vaccine against pasteurellosis of fur animals is a harmless and areactogenic preparation. This vaccine does not render negative action on hematological and biochemical indicators of mink blood. They are identical to indicators in control group of fur animals. Immunization with this vaccine stimulates cellular and humoral immunity factors that testifies to formation of the proof immune answer | Приведены данные о влиянии разработанной отечественной инактивированной вакцины против пастереллеза пушных зверей на гематологические, биохимические, иммунологические показатели норок. Установлено, что применение данного препарата ведет к формированию необходимого иммунного ответа. Разработанная отечественная инактивированная вакцина против пастереллеза пушных зверей является безвредным и ареактогенным препаратом. Инактивированная вакцина против пастереллеза пушных зверей не оказывает отрицательного действия на гематологические и биохимические показатели крови норок. Они идентичны показателям в контрольной группе пушных зверей. Иммунизация инактивированной вакциной против пастереллеза пушных зверей стимулирует клеточные и гуморальные факторы иммунитета, что свидетельствует о формировании стойкого иммунного ответа

There was studied the changes of the general protein content, albuminous fractions, antibodies and leukogram in blood of boars at influence by the laser on biologically active points. At laser influence on biologically active points, which are situated in lumber and sacral section spine, is taken place stimulating of body resistance. In the experiences the influence by the laser with various frequency has led to authentic increase in the content of protein in blood at 512 Hz with 75,9 to 81,2 and at 4046 with 75,4 to 80,8 g/l. The percent of eosinocytes in blood which carry out antitoxic function increases - from 2,63 to 4,85% and according to 2,50 to 5,15% at P0,01. Influence by laser radiation has caused number increase limfotsits in blood from 44,38 to 50,13% and according from 45,38 to 49,38% at P0,01. In the research the analysis of the content of antibodies in blood of animals was carried out. It was established that after processing by the laser (P0,05) the content in blood of immunoglobulin A increases at frequency of influence of 512 Hz from 2,2 to 3,2 g/l and at frequency of 4046 Hz from 2,4 to 3,3 g/l. | Цель работы заключалась в изучении изменения содержания общего белка, белковых фракции, иммуноглобулинов и лейкограммы в крови хряков при воздействии лазером на биологически активные точки. При воздействии лазером на биологически активные точки, расположенные в поясничном и крестцовом отделе позвоночника, происходит стимуляция резистентности организма. В проведенных опытах воздействие лазером с различной частотой привело к достоверному увеличению содержания белка в крови при 512 Гц с 75,9 до 81,2 и при 4046 с 75,4 до 80,8 г/л. Процент эозинофилов в крови, которые выполняют антитоксическую функцию, увеличивается с 2,63 до 4,85% и соответственно с 2,50 до 5,15% при Р0,01. Воздействие лазерным излучением вызвало увеличение числа лимфоцитов в крови с 44,38 до 50,13% и соответственно с 45,38 до 49,38% при Р0,01. В исследованиях проведен анализ содержания иммуноглобулинов в крови животных. Установлено, что после обработки лазером достоверно (Р0,05) увеличивается содержание в крови иммуноглобулина А при частоте воздействия 512 Гц с 2,2 до 3,2 г/л и при частоте 4046 Гц с 2,4 до 3,3 г/л.