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Sources of variation introduced into a phagocytosis assay as a result of the isolation of neutrophils from bovine blood.
1988
Paape M.J. | Miller R.H.
A study was conducted to examine sources of variation introduced into a phagocytosis assay as a result of the isolation of neutrophils from bovine blood, including variation attributable to isolation of neutrophils from blood, variation between duplicate determinations of percentage phagocytosis, and the variation in the ability of neutrophils isolated from blood (over repeated collections from the jugular vein) to phagocytose. For the phagocytosis assay, jugular venous blood from each of 4 cows was divided into 2 equal portions. The neutrophils were isolated by lysis of red blood cells with 0.2% sodium chloride. The neutrophils (2 X 10(7)) were incubated in duplicate with 32P-labeled Staphylococcus aureus ([32P]SA; 2 X 10(8)) inskimmed milk samples (2.5% final concentration) prepared from 4 cows. This process was repeated thrice on neutrophils isolated from 4 cows at 2-week intervals. The proportions of variation in percentage of 32P-labeled S aureus phagocytosed between duplicate neutrophil isolations and between duplicate assay determinations were 0 and 1%. Differences among skimmed milk sources and among runs, using blood neutrophils taken at different times from the same donor cow, accounted for 62 and 36% of the total variation. The results indicated that variation arising from blood neutrophil isolation introduced into a phagocytosis assay within a single-day trial is of no concern. The large variation among skimmed milk sample sources indicated differences among cows in the ability of their milk to support phagocytosis. The variation in neutrophil isolations over time for any cow was considered too large to allow for evaluation of physiologic and environmental effects on phagocytosis of neutrophils isolated from blood.
Show more [+] Less [-]Isolation of an adeno virus from hydropericardium syndrome in broiler chicks.
1988
Khawaja D.A. | Ahmad S. | Rauf A.M. | Zulfiqar M. | Mahmood S.M.I. | Mahmood ul Hassan M.
Incidence of Bordetella bronchiseptica infection in Youngnam swine herds and the biochemical properties of the organisms recovered from pigs with atrophic rhinitis.
1988
Jang H.K. | Kim B.H.
The present study was conducted to investigate the Bordetella bronchiseptica infection in Youngnam swine herds during the period of August 1986 to July 1987 and some properties of the organisms isolated from these Korean swine. B. bronchiseptica was recovered from 25 of 70 (35.7%) growing pigs of 4 to 10 weeks of age and from 12 of 13 (92.3%) herds. From 115 slaughter pigs, 58(50.4%) pigs were culture positive and the pigs from 13 of 14 (92.9%) herds were found to be infected with B. bronchiseptica. The majority of biochemical and cultural properties of B. bronchiseptica isolated from Korean swine were identical to those of the standard strain employed and some 97.6% of the isolates showed the characters of phase I organism on primary isolation.
Show more [+] Less [-]Studies on Aujeszky's disease in Korea: 1. isolation and characterization of the agent from infected pigs.
1988
Lee J.B. | An S.H. | Kim B.H. | Song J.Y. | Kim Y.H. | Sul D.S.
The first outbreak of aujeszky's disease (AD) was identified from piggery located at the southern part of Korea in July, 1987. This piggery suffered from a significant economic loss caused by unexpected piglet mortality and reproductive failure. Etiologic viral agents were isolated from tonsil and spleen of the infected piglets, and the isolates produced a typical cytopathic effect of herpesvirus with giant cell formation when inoculated in many different cells. Subsequently the field isolates were characterized as suid herpesvirus I by cross-neutralization test and indirect fluorescence assay utilizing specific monoclonal antibody, and proved to be a pathogenic strain of AD virus(ADV).
Show more [+] Less [-]Perfect state of Microsporum gypseum complex isolated from animals and soils.
1988
Lee H.J. | Choi W.P.
Mating experiments were performed to elucidate the distribution of perfect state of Microsporum gypseum complex originating from animals and soils in Korea. A total of 30 strains of M. gypseum complex, composed of 16 from animals and 14 from soils, mated with the tester strains of Nannizzia incurvata, N. gypsea and N. fulva. Among 30 strains of M. gypseum complex examined, 16 strains (53.3%) were N. incurvata, 13 strains (43.3%) N. gypsea and 1 strain (3.3%) N. fulva. Among 13 strains of dog isolates, 8 strains (61.5%) were N. incurvata and 5 strains (38.5%) N. gypsea. And each strain isolated from Korean native goat, monkey and albino rat was N. gypsea. Among 14 strains of soil isolates, 8 strains (57.2%) were N. incurvata, 5 strains (35.7%) N. gypsea and 1 strain (7.2%) N. fulva. And - mating type was more frequently obseved than + mating type in the strains of N. incurvata and N. gypsea. By this study, N. fulva was isolated the first time in Korea.
Show more [+] Less [-]Genetic properties of R plasmids in Salmonella isolates of swine and bovine origin in Korea, 1. Distribution and drug resistance of Salmonella isolated from dairy cow.
1988
Choi W.P. | Lee H.S. | Chae T.C. | Yeo S.G. | Lee H.J.
This paper deals with the distribution of Salmonella (S) infection on 4 herds in Kyungju and Taegu during the period from May to October 1986. Isolated Salmonella were examined for serotypes, antimicrobial drug resistance and detection of R plasmid. From 4 herds, 67 Salmonella were isolated from 51 samples (1.1%), and their serovar strains were S. typhimurium 6, S. derby 5, S. infantis 4, S. bareilly 4, S. dublin 3, S. anatum 2, S. montevideo 2 and untypable 41. In 4 herds, the incidence of drug resistance was 57.7-100% and transfer frequency of conjugative R plasmid was 96.1-100%.
Show more [+] Less [-]Studies on the pathogenesis and interspecies transmission of respiratory syncytial virus isolated from sheep
1988
Bryson, D.G. | Evermann, J.F. | Liggitt, H.D. | Foreyt, W.J. | Breeze, R.G.
Inoculation of lambs with an ovine isolate of respiratory syncytial virus (RSV) by a combined intranasal and intratracheal route resulted in mild respiratory tract illness, with respiratory tract lesions. Lung lesions were characterized by bronchitis and bronchiolitis, hyperplasia of bronchial and bronchiolar epithelium, peribronchiolar and perivascular accumulations of lymphocytes, alveolar and perivascular accumulations of lymphocytes, alveolar septal thickening, and collapse. Respiratory synctial virus was recovered from the respiratory tract of inoculated lambs, and RSV antigen was demonstrated by immunoperoxidase staining of bronchiolar and alveolar epithelial peroxidase staining of bronchiolar and alveolar epithelia cells in pneumonic lesions of lambs euthanatized on post-inoculation days 5 and 6. Other primary respiratory tract pathogens were not isolated. Clinical signs of respiratory tract illness or respiratory tract lesions did not develop in the in-contact control lamb. Inoculation of the ovine RSV isolate into calves and deer fawns resulted in infection in both species, and at necropsy, pneumonic lesions were present. A mild to moderate respiratory tract illness developed in the calves, but clinical disease was not seen in the fawns. Lung lesions in fawns were similar to those seen in lambs; lesions in calves were characterized by collapse, scattered areas of parenchymal necrosis, and bronchiolitis. Respiratory synctial virus was reisolated from the lower respiratory tract of inoculated calves and fawns, and immunoperoxidase positive epithelial cells were seen in pneumonic lesions Other primary respiratory pathogens were not detected. Respiratory syncytial virus infection was not demonstrable in control animals that were in contact with inoculated animals. We concluded that an ovine RSV isolate, when inoculated in a severe challenge regime, caused mild primary pneumonia in lambs and lesions similar to those described in epizootics of naturally occurring ovine respiratory tract disease. Also, the ovine RSV caused lower respiratory tract lesions in infected calves and deer.
Show more [+] Less [-]Isolation and characterization of epizootic hemorrhagic disease virus from sheep and cattle in Colorado
1988
Thompson, L.H. | Mecham, J.O. | Holbrook, F.R.
Epizootic hemorrhagic disease virus was isolated from cattle and sheep in northeastern Colorado during July and August 1984. The isolates were identified as serotype 2 by plaque-inhibition serotyping, genome electropherotyping, and protein analysis.
Show more [+] Less [-]Prevalence of fimbrial antigens and enterotoxins in nonclassical serogroups of Escherichia coli isolated from newborn pigs with diarrhea
1988
Fairbrother, J.M. | Lariviere, S. | Johnson, W.M.
Ninety-nine nonclassical serogroups isolated from newborn pigs with neonatal diarrhea were tested for fimbrial antigens F4(K88), F5(K99), F6(987P), F41, and F165, and for heat-labile enterotoxin, heat-stable enterotoxin a (STa), heat-stable enterotoxin b, verocytotoxin, and cytolethal-distending toxin. Thirty-two strains, belonging mostly to serogroups O64:K"V142,", O9:K103, and O20:K101, were F5-positive and usually produced STa, although 5 strains produced only heat-stable enterotoxin b. Fifteen strains, belonging mostly to serogroups O64:K"V142" and O20:K101, were F41 positive and usually produced STa. Twenty-three strains belonging mostly to serogroups O64:K"V142," O9:K103, and O20:K101, were F6-positive and usually produced STa. Several strains produced more than one fimbrial antigen, either F5 and F41, F5 and F6, F6 and F41, F6 and F165, or F5, F6, F41, and F165. None of the strains produced heat-labile enterotoxin, verocytotoxin, or cytolethal-distending toxin. The indirect immunofluorescence test was much more sensitive than was the slide agglutination test for the detection of each of the fimbrial antigens F5, F6, F41, and F165 on strains grown in vitro. The production of F6 by certain strains for which only a low proportion of cells were F6-positive in vitro as demonstrated by immunofluorescence, was confirmed by experimental infection of newborn pigs.
Show more [+] Less [-]Sources of variation introduced into a phagocytosis assay as a result of the isolation of neutrophils from bovine blood
1988
Paape, M.J. | Miller, R.H.
A study was conducted to examine sources of variation introduced into a phagocytosis assay as a result of the isolation of neutrophils from bovine blood, including variation attributable to isolation of neutrophils from blood, variation between duplicate determinations of percentage phagocytosis, and the variation in the ability of neutrophils isolated from blood (over repeated collections from the jugular vein) to phagocytose. For the phagocytosis assay, jugular venous blood from each of 4 cows was divided into 2 equal portions. The neutrophils were isolated by lysis of red blood cells with 0.2% sodium chloride. The neutrophils (2 X 10(7)) were incubated in duplicate with 32P-labeled Staphylococcus aureus ([32P]SA; 2 X 10(8)) inskimmed milk samples (2.5% final concentration) prepared from 4 cows. This process was repeated thrice on neutrophils isolated from 4 cows at 2-week intervals. The proportions of variation in percentage of 32P-labeled S aureus phagocytosed between duplicate neutrophil isolations and between duplicate assay determinations were 0 and 1%. Differences among skimmed milk sources and among runs, using blood neutrophils taken at different times from the same donor cow, accounted for 62 and 36% of the total variation. The results indicated that variation arising from blood neutrophil isolation introduced into a phagocytosis assay within a single-day trial is of no concern. The large variation among skimmed milk sample sources indicated differences among cows in the ability of their milk to support phagocytosis. The variation in neutrophil isolations over time for any cow was considered too large to allow for evaluation of physiologic and environmental effects on phagocytosis of neutrophils isolated from blood.
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