Mesenchymal stem cells (MSCs) have ability to differentiate into multi-lineage cells, which confer a great promise for regenerative medicine to the cells. The aim of this study was to establish a method for isolation and characterization of adipose tissue-derived MSC (pAD-MSC) and bone marrow-derived MSC (pBM-MSC) in pigs. Isolated cells from all tissues were positive for CD29, CD44, CD90 and CD105, but negative for hematopoietic stem cell associated markers, CD45. In addition, the cells expressed the transcription factors, such as Oct4, Sox2, and Nanog by RT-PCR. pAD-MSC and pBM-MSC at early passage successfully differentiated into chondrocytes, osteocytes and adipocytes. Collectively, pig AD-MSC and BM-MSC with multipotency were optimized in our study.

Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.

A total of 593 mastitic pathogens was isolated from the clinical mastitic milk of dairy cattle in Kyonggi area from March 1984 to February 1988. The mastitic pathogens were further studied bacteriologically and examined for susceptibility patterns to 10 antibiotics. 173 pathogens were isolated in the first year of studies, 205 pathogens in the second year, 122 pathogens in the third year and 93 pathogens in the last year. The pathogens isolated from clinical mastitis were more in summer than other seasons. Staphylococcus spp (50%) and Streptococcus spp (18%) were the main pathogens in the first year of studies but coliforms (15%) and other bacteria (40%) were the main pathogens in the last year of studies. Coliform mastitis occurred only in summer and autumn. Antibiotic susceptibility patterns of the same genuses from clinical mastitis infections in different herds and in different years varied greatly. Therefore, treatment should be selected on the basis of susceptibility test results

A total of 108 strains of C jejuni isolated from pigs and chickens were examined for the susceptibility to 10 antimicrobial agents and normal sera of cattle, sheep, guinea pigs and chickens. Minimal inhibitory concentration (MIC) ranges of antimicrobial agents to C jejuni isolates were = 100 micro g/ml for erythromycin, rifamycin, streptomycin and tetracycline, 50 to 100 micro g for cephalothin, = 50 micro g for ampicillin,

The present study was conducted to investigate the incidence of Pasteruella multocida infection in Youngnam swine herds during the period from March 1988 to Ferbruary 1989 and some properties of the isolated organisms. P multocida was isolated from 22 (43.1 %) of 51 growing pigs of 4 to 12 weeks of age and from 8 (80.0 %) of 10 herds. From nasal turbinates of 102 slaughtered pigs, 47 (46.1 %) pigs were culture positive and pigs from 8 (88.9 %) of 9 heards were found to be infected with P multocida. From lungs of 101 slaughtered pigs, 42 (41.6 %) pigs were culture positive and the pigs from 11 (91.7 %) of 12 herds were found to be infected with P multocida. The majority of biochemical and cultural propertis of the P multocida isolates were identical to those of the standard strains. The isolation frequencies of P multocida in relation to pig snout lesion grades of 0 to 5 were 28.6 %, 41.6 %, 48.0 %, 50.0 % 85.7 %, and 100 %, respectively

This paper deals with the 0 groups of citrate utilizing variants of Escherichia coli (Cit+ E. Coli) isolated from cattle, the production of colicin, hemolysin, K99 antigen, heat stable enterotoxin, and the isolation of plasmid DNA. Among 42 Cit + E. Coli, 12 strains were 020, 9 strains 08, 5 strains 045, 3 strains 0115, 1 strain 064, 1 strain 0139 and remaining strains (11) were untypable. Thirty-nine(81.3%)out of 48 Cit + E. Coli produced colicin and 13(27.0%) produced hemolysin. Of 12 cit + E. coli bearing K99 antigen, 6(50.0%) produced heat stable enterotoxin. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmids varied from 1 to 7 in 10 Cit+ E. Coli. Its molecular weight ranged from 2 to 50 Mdalton, and 50 Mdalton plasmid commonly existed in all strains

Fecal material from cattle, which was confirmed to be infected with Johne's disease by clinical and pathological symptoms, was decontaminated with 4% NaOH and inoculated into the Lowenstein-Jensen media supplemented with 1% of heat-killed Mycobacterium bovis. After 2-4 week-incubation at 37 deg C, typical acid-fast mycobacteria was isolated. The requirements of mycobactin for growth and the other biochemical properties, isolated mycobacteria was identified as Mycobacterium paratuberculosis

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campylobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Camplyobacter spp. were examined. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60 % of the isolates were noted to harbor plasmid DNA. Plasmid occurrence rate from pigs, chickens and cattle were 76.2 %, 61.7 % and 37.7 %, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10-1,000. Tetracycline resistant plasmids of C jejuni were transferarble to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E. coli and Vibrio cholerae. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation

The present study was carried out to investigate C. jejuni and C. coli in chicken. Also were examined the pathogenicity of the isolates in chicks by experimental inoculation. Thermophilic Campylobacter were isolated from 34 (45.9%) of the 74 specimens, and classified as 21.6 % C. jejuni, and 24.3 % C. coli. In the biotyping of 16 strains of C. jejuni isolates, 37.5 % of the strains were grouped as biotype I, 62.5 % as biotype II. In the case of 18 strains of C. coli isolates, 49.9 % of isolates were grouped as biotype I, 55.6 % as biotype II. In oral inoculation with 10** (4)cfu of Capmylobacter isolates into infant chicks (1 to 3 days-old), 17 days-old and 34 days-old chicks, 32.5 % of the chicks developed diarrhea on day 1, 52.5 % on day 3, 70.0 % on day 5, and 27.5% on day 7, and the peak incidence of diarrhea was reached on day 5. The organisms were found to be discharged in feces one day afterwards. C. jejuni and C. coli strains were detected from the feces in 87.5 % of the chicks on day 5.

Serological study and isolation of Brucellae from dairy cattle were carried out in Kyungbook area of Korea during the period from 1984 to 1985. Biotyping of the isolates was done. Of the 11168 cattle in 2479 herds examined, 27 cattle (0.24%) in the 17 herds (o.69%) were serologically positive for brucellosis. Brucella abortus was recovered from 20 (74.1%) of the 27 infected cattle, and all the strains of the isolates belonged to biotype 1. Of the tissues cultured for brucellae, the supramammary lymph nodes had the highest rate (55.6%) of recovery of brucella organisms