BACKGROUND: Biological control of parasites by using entomopathogen fungi is the one of the recommended ways to control them instead of using the chemical agents. Entomopathogen fungi are not pathogenic for animals and plants, while ticks are one of the most important parasites of animals that can transmit very important microbial pathogens. Ixodes ricinus is a hard tick that infests animals and human. OBJECTIVES: This study demonstrated enzyme assay of entomopathogen fungi hosted on Ixodes ricinus.  METHODS: Enzymatic activities of chitinase, lipase and protease of fungal structures on the killed tick bodies have been assayed by standard sphectrophotometric methods.  RESULTS: Chitinase, lipase and protease activities showed significant differences among different fungal strains (p<0.05). This research, which was done for first time in Iran demonstrated the effect of some enzymes which affect on acaricidal  properties of native strain of entomopathogenic fungi  in Iran. CONCLUSIONS: This study reveals the relationship between enzyme level of fungal strains and  the possibility of selecting more effective strains of entomopathogenic fungi

Lyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks. A total of 136 Ixodes ricinus ticks, collected in the Ternopil (Ukraine) region, including 126 adults (70 females and 56 males), and 10 nymphs were examined. The identification of the species and their developmental form was based on morphological characteristics. PCR with B5S-Bor and 23S-Bor primers resulted in Borrelia burgdorferi sensu lato DNA amplification among six ticks (4.4%). The detailed analysis based on the DNA sequencing showed the presence of DNA of Borrelia afzelii in four samples; the remaining two represented Borrelia burgdorferi sensu lato complex, although their genospecies were not determined. The research confirmed the dominance of Borrelia afzelii genospecies in the ticks from Ukraine. It seems reasonable to undertake similar research in ticks from other regions of Ukraine. Knowledge in this field can be useful for public health and planning the prevention of tick-borne diseases.