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Changes in the serum immunoglobulin levels and viral antibody titers of colostrum-conferred Korean native calves during the first 12 weeks postpartum.
1989
Kim D. | Han H.R.
The changes in serum total protein and immunoglobulin levels, and BVD, IBR and PI-3 viral neutralizing antibody titers in colostrum-conferred Korean native calves during the first 12 weeks postpartum were studied. The mean concentration of total protein, total immunoglobulin, IgG, IgM and IgA in sera of 9 calves at birth were 3.8 +- 0.5g/dl, 0.27 +- 0.15mg/ml, 0.06 +- 0.08mg/ml, 0.21 +- 0.11mg/ml, and extremely low concentration, respectively. Serum total protein level reached a maximum at 20 hours after birth, total immunoglobulin, IgG and IgM levels at 24 hours, and IgA level at 28 hours, respectively. Serum IgA level reached a minimum at 4 weeks old, IgM level at 5 weeks, total immunoglobulin level at 8 weeks, and IgG level at 10 weeks, respectively. After then those levels had begun to increase, but total protein level was still decreasing at 12 weeks old. The half-lives of IgG, IgM, and IgA were 21.1 days, 4.0 days, and 2.6 days respectively. In 10 Korean native cows immediately after parturition, serum neutralizing antibody titers specific to BVD, IBR and PI-3 virus were 8.7 +- 1.5 log2, 5.7 +-1.2 log2, and 6.8 +- 1.0 log2, respectively. And colostral neutralizing antibody titers against BVD, IBR, and PI-3 virus were 10.1 +- 1.4 log2, 6.8 +- 1.3 log2, and 7.8 +- 1.7 log2, respectively. Before suckling the colostrum, SN antibody titers against BVD, IBR, and PI-3 virus were undetectable from all of 9 Korean native calves. Nevertheless SN antibody titer against BVD virus reached a maximum level (9.2 +- 0.6 log2) at 24 hours after birth, that against IBR virus (6.1 +- 1.0 log2) at 20 hours after birth, and that against PI-3 virus (6.8 +- 0.9 log2) at 32 hours after birth, respectively. In 12 weeks old calves, the SN antibodies against BVD and IBR virus were still decreasing, but that against PI-3 virus reached a minimum at 10 weeks, and increased after 12 weeks of age. The half-lives of SN antibodies against BVD, PI-3 and IBR, virus were 16.0 days, 22.6 days, and 25.5 days, respectively.
Show more [+] Less [-]Clinicopathological studies on the subclinical fascioliasis in the Korean native cows in Chonnam area.
1989
Lee C.G. | Wee S.H. | Park S.J.
Fecal samples were taken from 402 cows in Posung, Chonnam which was designated as a place for Korean native cattle breeding. Prevalence of internal parasitisms were determined by the fecal examinations using the floatation and sedimentation procedures. 62.9 % of the cows were found as positive cases with excretion of the eggs of Fasciola hepatica in the fecal specimens. Of those infected with F. hepatica 97 cows free of other pathogenic intestinal parasites were chosen for albendazole treatment. Albendazole tablets (10mg/Kg) were administered to the cows twice at the interval of 4 weeks. Blood samples were collected via jugular vein prior to the first treatment, four weeks after the first treatment and four weeks after the second treatment, respectively. At the same time fecal samples were collected for parasitological examinations by sedimentation methods. The mean pretreatment count was 44 fluke eggs per gram of feces, which compared with 27 epg and 17 epg four weeks after the first and second treatment, respectively. Most of the hematological and biochemical values fluctuated within the normal ranges during the experiment. Eosinophil counts were high initially, decreased after the first treatment and thereafter remained steady. The opposite was the case with aspartate and alanine aminotransferases.
Show more [+] Less [-]Genetic properties of R plasmids in Salmonella isolates of swine and bovine origin in Korea., 2; incompatibility and profile of R plasmid.
1989
Choi W.P. | Lee H.S. | Yeo S.G. | Lee H.J. | Jung S.C.
This paper deals with the genetic properties of R plasmids in Salmonella originated from pigs and cattle. The plasmid DNA was examined for incompatibility, stability and fertility inhibition (F1), and gel electrophoresis was performed for isolation of plasmid DNA. Among the 66 conjugative R plasmids from 44 pigs and 22 cattle, 61 R plasmids (92.4 %) were Fi-, whereas the remainder were Fi+. The Inc groups of 66 R plasmids were determined with 7 standard plasmids. Twenty-six R plasmids were classified into Inc group Ialpha, H1, H2 or F1, 40 R plasmids being not classified with standard plasmids used, and the Inc group Ialpha (57.7 %) was most frequent. 3. Inc groups Ialpha H1, and F1 were identified in strains from swine, Inc groups H2 and F1 from cattle. The plasmid DNA profiles in 16 Salmonella isolated from pigs and cattle were confirmed as being 1 to 10 fragments by the gel eletrophoresis. Their molecular weight ranged 1.0 to 90 megadalton. The molecular weight of conjugative plasmids ranged 1.0 to 80 megadalton in 4 Salmonella (P-4, P-5, P-7 and P-8) isolated from pigs.
Show more [+] Less [-]Genetic properties of R plasmids in Salmonella isolates of swine and bovine origin in Korea, 1. Distribution and drug resistance of Salmonella isolated from dairy cow.
1988
Choi W.P. | Lee H.S. | Chae T.C. | Yeo S.G. | Lee H.J.
This paper deals with the distribution of Salmonella (S) infection on 4 herds in Kyungju and Taegu during the period from May to October 1986. Isolated Salmonella were examined for serotypes, antimicrobial drug resistance and detection of R plasmid. From 4 herds, 67 Salmonella were isolated from 51 samples (1.1%), and their serovar strains were S. typhimurium 6, S. derby 5, S. infantis 4, S. bareilly 4, S. dublin 3, S. anatum 2, S. montevideo 2 and untypable 41. In 4 herds, the incidence of drug resistance was 57.7-100% and transfer frequency of conjugative R plasmid was 96.1-100%.
Show more [+] Less [-]Course and distribution of facial nerve of the Korean native goat.
1986
Lee H.S. | Lee I.S. | Kim D.J.
This study was carried out to investigate the branch and distribution of Nervus facialis of the Korean native goat. The observation was made by dissection of embalmed cadavers of ten Korean native goats. N. facialis arose from the ventrolateral surface of the medulla oblongata. In the facial canal, N. facialis gave off N. petrosus major, N. stapedius and Chorda tympani. N. petrosus major arose from Ganglion geniculi, passed through the pterygoid canal and terminated in Ganglion pterygopalatium. Chorda tympani joined N. lingualis at the lateral surface of the internal pterygoid muscle.
Show more [+] Less [-]The number and distribution of retinal ganglion cells in a Korean native cattle.
1989
Kim M.K. | Cho S.W. | Ryu S.Y. | Kim K.J. | Kim S.K. | Shin T.K. | Lee G.I.
The number and distribution of the retinal ganglion cells in the 2 years old Korean native cattle was determined from whole flat mounted preparation stained with methylene blue and thionin. The total number of retinal ganglion cells was estimated to be 3,085,200 in the bovine retina ranging from 2.214mm** (2) in total area. Visual streak was recognized at the area 2.5mm superior to the optic disc and ganglion cell density drops off rapidly to the direction superior to and inferior to the visual streak. Area centralis (6,800 cells/mm** (2)) was located at the area 10mm temporally from the point of 3mm superior to the optic disc. The number of alpha-type ganglion cells (above 15 micro) was 57,000 in the bovine retina and alpha-type ganglion cells constituted 18.5 % of the total cells. The relative frequency of alpha-type ganglion cells was higher in the peripheral regions than in the visual streak, especially higher in the superior-temporal quadrant than in other region of the bovine retina.
Show more [+] Less [-]A morphological study on the sternal development of Korean cattle.
1989
Lee H.K. | Yang H.H. | Paik Y.K.
This study was undertaken to obtain basic data of the sternal development in Korean native cattle from the earliest sternal formation to the ossification using histological and histochemical methods. Thirty three sterna were collected from a series of embryos and fetuses ranging from 11 to 225mm (estimated age 37-120 days) in crown rump length. The bilateral sternal bars were observed in the 2nd group (CRL 21-30mm) of Korean cattle embryos. Those bars initiated to be fused in the 3rd group (CRL 31-40mm) and completed in the 7th group (CRL 71-80mm). The ossification centers were detected in the 8th group (CRL 81-90mm) also bilateral ossification centers were found in the same group. The typical epiphyseal plates, endochondral bone and calcium deposit were found in the 9th group (CRL 91-100mm). Osteocytes, osteoblasts, osteoclasts and myeloid cells appeared in ossification centers in the 10th group (more than CRL 101mm). The alcianophility responded markedly in the 9th group that was decreased and showed slightly positive reaction in territorial matrix of the 10th group. Marked positive reaction to PAS was observed in bony trabeculae in the 10th group. The positive reaction to calcium deposit by trichrome stain was observed initially in the hypertrophied zone of epiphyseal plate in the 9th group and was conspicuous in the calcified zone of epiphyseal plate in the 10th group. The 1st positive reaction to the von Kossa stain was observed in the 9th group.
Show more [+] Less [-]Antimicrobial resistance and transfer of R plasmid of pathogenic Escherichia coli isolated from poultry in Korea
2008
Sung, M.S. (Kyungpook National University, Daegu, Republic of Korea) | Kim, J.H. (Kyungpook National University, Daegu, Republic of Korea) | Cho, J.K. (Health and Environmental Research Institute, Daegu, Republic of Korea) | Seol, S.Y. (Kyungpook National University School of Medicine, Daegu, Republic of Korea) | Kim, K.S. (Kyungpook National University, Daegu, Republic of Korea), E-mail: kimkiseuk@knu.ac.kr
Antimicrobial drugs are widely used in poultry industry as growth promoters or to control infectious diseases. However, this practice is reported to have caused high resistance to antimicrobial drugs in normal chicken flora and pathogens. Antimicrobial resistance to Escherichia coli (E. coli) from chicken has been mainly reported in normal flora, but rare in pathogenic organism in Korea, recently. Therefore, this study was conducted to investigate prevalence of antimicrobials resistance, transfer of R plasmid, and association between antimicrobial drug resistance and O serotype of 203 pathogenic E. coli from poultry in Korea during the period from April 2003 to December 2005. These isolate showed a high resistance to tetracycline (Tc, 92.6%), streptomycin (Sm. 81.8%), ampicillin (Ap, 77.3%), ciprofloxacin (Ci, 70.9%), sulfisoxazole (Su, 66.5%), and trimethoprim (Tp, 58.1%). Two hundred-one (99.0%) of the isolates were resistant to one or more drugs. They showed 57 different resistant patterns, and the most prevalent resistant pattern among them was Tc, Sm, Su, Ap, Tp, Ci, Na. Sixty-eight (33.8%) of the isolates transferred all or a part of their antimicrobial resistant pattern to the recipient strain by R plasmid. The most common antinicrobial resistant pattern was Tc, Sm, Su, Ap, Tp, Ci, Na in serotype O78, O88 and O15, respectively. These results exhibit high individual and multiple resistance to antimicrobials of pathogenic E. coli from poultry in Korea. They also suggest the needs for surveillance to monitor antimicrobial resistance in pathogenic bacteria that can be potentially transmitted to humans from food animals and to regulate the abuse of antimicrobials in food-producing animals in Korea.
Show more [+] Less [-]Descriptive epidemiology of nationwide spread of 2003 classical swine fever epidemic in Korea
2006
Park, C.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: parkck@nvrqs.go.kr | Lee, E.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, H.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Wee, S.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Moon, O.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Choi, E.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, H.S. (Chungnam National University, Daejeon, Republic of Korea) | Lee, J.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | An, S.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
In March 2003, classical swine fever (CSF) infection was reported in a piggery located at Iksan city, Jeollabuk-do in Korea. Subsequently, a total of 72 infected farms were confirmed between March and December, 2003. Based on epidemiological investigation of the earlier confirmed infected farms, the source of infection was shown to be from a breeding farm. Targeted surveillance of 82 piggeries that had acquired pigs from this breeding farm showed 44 piggeries were infected with CSF virus.
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