Afeeding trial was conducted in layers for a period of8 weeks (23-30 weeks old) to evaluate the effect of chromium picolinate supplementation on egg yolk cholesterol and egg chromium content in layers.Atotal of (n=120) layers were randomly distributed in completely randomized design into 4 treatmentgroupseachwith2 replicates of 5chicks.The layers of treatment group T1(control)were provided water without chromiumwhile those of T2,T3andT4were provided water containing 200, 400 and 600 ppb chromium / litre respectively.At the end of feeding trial, six eggs from each replicate, on last three days were used for egg yolk cholesterol and egg chromium studies. The results indicated that the total cholesterol andLDL cholesterol content of eggs were significantly (P0.05) reduced and HDL-cholesterol content significantly increased in 400 and 600 ppb chromium supplemented group of layers. Whereas chromium content of eggs increased with increasing levels of chromium supplementation. It was concluded that organic chromium supplementation up to 600 ppb level through water may be advisable to reduce egg yolk total cholesterol and LDL- cholesterol and improved HDL-cholesterol and egg chromium content.

This study was conducted to ascertain anatomical variation in different body parts of Desi, Fayoumi, Cross (Rhode Island Red x Fayoumi) and Nick Chick laying hens. A total 16 laying hens of average body weight were selected using random numbers out of 2600 laying hens, slaughtered and eviscerated. It was observed that proportion of neck, ribs, breast, back, wings, thigh and legs out of aggregate weight was highest (51.74 plus minus 2.85) in Fayoumi hens. The proportion of liver, gizzard, heart and spleen combined weight was highest (6.05 plus minus 1.81) in desi hens. The proportion of non edible offals including trachea, lungs, kidneys, blood, feathers, head, crop, proventriculus, intestines, shanks, gizzard waste, skin, tail, testes, ova, oviduct and fat was estimated to be highest i.e. 46.60 plus minus 1.16 in Nick Chick laying hens. The anatomical variation in Desi and Fayoumi chicks was attributable to scavenging habit of these birds. The high proportion of edible offal in commercial Nick Chick hens may be ascribable to higher productive and reproductive traits.

Objective: Infectious laryngotracheitis virus (ILTV) is responsible for causing infectious laryngo¬tracheitis (ILT), which is a rapidly spreading and extremely transmissible disease in chickens. The current research aims to isolate and characterize ILTV from layer chickens in Bangladesh. Materials and Methods: A total of 345 samples (trachea, larynx, and lungs) were collected from ILT-suspected dead and sick layer chickens of 32 ILT-suspected farms in three different outbreak districts (Gazipur, Tangail, and Mymensingh) of Bangladesh during the outbreak year 2021-2022. Rapid detection kits examined the samples for avian influenza virus (AIV) and Newcastle disease virus (NDV). ILTV-specific primers were used to screen 72 NDV- and AIV-negative samples by poly¬merase chain reaction (PCR). Using chorioallantoic membrane (CAM), the study isolated the ILT virus from 9 to 10-day-old seronegative embryonated chicken eggs (ECEs) using selected PCR-positive samples. The virus was confirmed using nucleotide sequencing, agar gel immunodiffusion test (AGIDT), viral neutralization test (VNT), and pathogenicity evaluations using mortality index for chicken embryos (MICEs) and intra-tracheal pathogenicity index (ITPI). Results: The results indicated that among the PCR-positive 10 samples, only two (Alim_ILT_1001 and Alim_ILT_1,000) were found positive using ECEs. There were two field isolates of ILTVs, as shown by the amplicon size of the ICP4 gene-based PCR. A phylogenetic study of the ICP4 gene revealed that the recent isolates have a close similarity with the ILTV isolates of Turkey, Bangladesh, and Australia. AGIDT revealed strong precipitation lines due to ILTV-specific antibod¬ies reacting with field viruses, while VNT neutralized both isolates with conventional ILTV antibod¬ies. The pathogenicity testing indicated that Alim_ILT_1001 had MICE and ITPI values of 0.77 and 0.63, whereas Alim_ILT_1,000 had 0.71 and 0.57. Conclusion: Both the ILTV isolates have similarities with the isolates of Turkey, Bangladesh, and Australia, and they are highly virulent for chickens. [J Adv Vet Anim Res 2024; 11(2.000): 398-407]

In the conditions of the Republic of Belarus there was determined the influence of multi-enzymatic veterinary drug preparations Vitazim (contained a complex of enzymes of carbohydrases: xilanase; cellulose; beta-glucanase) and Ehkosim (universal multi-enzymatic complex: beta-glucanase; endo-xilase; and cellulase) on microbial composition of intestinal bacterial population. Research was realized by an example of layer hens of four-linear cross Highsex white at the age of 34-51 weeks. Regimens of administration of the analyzed preparations were presented. Research results showed promoted the development of the regenerative process of physiological media in intestines of poultry which was necessary for subsisting of normal flora of pathogenic bacteria. The studied preparations Vitazim and Ehkosim can be applied for the preventive treatment and therapy of intestinal dysbacteriosis, normalization of its microbial flora, as well as for the antibacterial therapy.

Scientific and production examination of feed additive Semerik-Vita containing iodine and selenium was realized in the conditions of the Republic of Belarus by studying of its influence on production and economic indexes of laying hens and their products, as well as by studying of its influence on clinical state and biochemical blood indexes of poultry. Research results showed that application of feed additive Semerik-Vita made it possible to increase of egg producing ability intensity on 1,8 %, egg yield - on 2,4 % in the conditions of lowering of feeding expenses on 1,4 %. Application of the analyzed feed additive made it possible to obtain a new kind of eggs fortified with iodine, selenium, beta-carotene and vitamins (A, E, C, PP, B1, B2, pantothenic acid and folic acid). In eggs of poultry of experimental group the content of selenium increased in 2,1 times, iodone - in 1,5 times, vitamin E – in 3,9 times, beta-carotene – in 1,4 times, vitamin A – in 1,3 times, pantothenic acid – in 1,2 times, vitamin C – in 1,17 times, vitamin PP – in 1,4 times, vitamin B1 – in 1,5 times, vitamin V2 – in 1,4 times, folic acid - in 1,5 times in comparison with eggs of laying hens from the control group. Research results proved that application of Semerik-Vita did not render negative effect on the clinical indexes of laying hens. It made it possible to lower the content of urea acid in 1,7 times, as well as to increase the content of triglycerides in 2 times, cholesterol - in 2,4 times, magnesium - in 1,28 times

For increase of efficiency and natural resistance, decrease in the cost price and increase of poultry efficiency the big attention is given to questions of bird mineral food. Now for balancing of bird rations on mineral substances, especially on calcium, the poultry-farming enterprises apply an expensive cockleshell which is delivered from Azov. During too time in our country there are enough of local sources of the mineral raw materials which use in bird feeding can be effective enough. Dolomite in the form of flour which stocks are great enough can serve one of sources of bird mineral food. As a result of the spent research it is established, that use of the local mineral additive dolomite in feeding of hens-layers probably as a substitute of an expensive import cockleshell. For an optimum dose it is possible to accept 3% of dolomite from forage mass. At use of the given dose the best results of egg efficiency, livestock safety and the expense of forages have been received. | Для повышения продуктивности и естественной резистентности, снижения себестоимости и повышения эффективности отрасли в целом большое внимание уделяется вопросам минерального питания птицы. В настоящее время для балансирования рационов птицы по минеральным веществам, особенно по кальцию, птицеводческие предприятия применяют дорогостоящую ракушку, завозимую с Азовского моря. В тоже время в нашей стране имеются достаточные количества местных источников минерального сырья, использование которых в кормлении птицы может быть достаточно эффективным. Одним из источников минерального питания птицы может служить доломит в виде муки, запасы которого достаточно велики. В результате проведенных исследований установлено, что использование местной минеральной добавки доломита в кормлении кур-несушек возможно в качестве заменителя дорогостоящей импортной ракушки. За оптимальную дозу можно принять 3% доломита от массы корма. При использовании данной дозы были получены лучшие результаты яичной продуктивности, сохранности поголовья и расхода кормов.