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Enhancement of Pasteurella haemolytica leukotoxic activity by bovine serum albumin.
1994
Waurzyniak B.J. | Clinkenbeard K.D. | Confer A.W. | Srikumaran S.
Growth of Pasteurella haemolytica A1 in RPMI 1640 medium containing 0.5% bovine serum albumin (BSA) for 2.5 hours enhanced culture supernatant leukotoxic activity [30,700 +/- 12,900 toxic units/ml, compared with leukotoxic activity of culture supernatants produced in RPMI 1640 medium alone (120 +/- 40 toxic units/ml)]. Gel filtration chromatography of the leukotoxic activity from RPMI 1640 medium supernatants in buffer containing 50 mM NaCl indicated a single leukotoxic activity peak (peak I) eluting near the gel resin molecular mass exclusion limit (estimated molecular mass of approx 8,000 kd). In contrast, culture supernatants produced in RPMI 1640 plus bovine serum albumin medium (RPMI + BSA) had peak I and 2 additional leukotoxic activity peaks (peaks II and III) with estimated molecular mass of approximately 80 and < 30 kd, respectively. All leukotoxic activity peaks were composed of approximately 100-kd molecular mass leukotoxin protomer, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with a monoclonal antibody against leukotoxin. Subjecting culture supernatant leukotoxic activity produced in RPMI + BSA to gel filtration chromatography in buffer containing 500 mM NaCl or 6M urea resulted in detection of only a single leukotoxic activity peak with estimated approximate molecular mass of 250 and 800 kd, respectively. These findings suggest that P haemolytica exists as a high molecular mass aggregate with low leukotoxic activity which, in the presence of BSA, partially disaggregates to multiple toxin forms with enhanced leukotoxic activity. Some of these leukotoxin forms interact with dextran-based gel resins at low ionic strength.
Show more [+] Less [-]Comparison of peritoneal fluid analysis before and after exploratory celiotomy and omentopexy in cattle.
1994
Anderson D.E. | Cornwell D. | St Jean G. | Desrochers A. | Anderson L.S.
The effect of right paralumbar fossa exploratory celiotomy and omentopexy on peritoneal fluid constituents was studied in 22 adult dairy cows. Six cows were eliminated on the basis of physical examination findings (n = 2), surgical findings (n = 2), or inability to obtain a sufficient volume of peritoneal fluid (n = 2). Sixteen cattle had normal results of Csc and serum biochemical analysis, and a minimum of 1 ml of peritoneal fluid was obtained by abdominocentesis. Abdominocentesis was repeated on days 1, 2, and 6 after surgery. Statistical analysis for repeated measures was performed, using a significance level of P < 0.05. Stage of gestation was evaluated for interaction with time. Mean total nucleated cell count was 3,200 cells/1 before surgery, was significantly increased 2 days after surgery (16,336 cells/microliter), and continued to increase through day 6 (20,542 cells/microliter). Mean polymorphonuclear cell count was 1,312 cells/microliter before surgery and was significantly higher at 2 (11,043 cells/microliter) and 6 (10,619 cells/microliter) days after surgery. Mean lymphocyte count was 254 cells/microliter before surgery and was significantly increased 2 days (1,911 cells/microliter) after surgery. By day 6, lymphocyte numbers were similar to preoperative values. Mean mononuclear cell count was 770 cells/microliter before surgery and was significantly increased on days 1 (3,084 cells/microliter), 2 (3,285 cells/microliter and 6 (2,349 cells/microliter) after surgery. Mean eosinophil numbers were 1,388 cells/microliter before surgery and were significantly increased on day 6 (6,347 cells/microliter) only. Interaction between time and stage of gestation was found only for specific gravity and total protein concentration. In general, specific gravity and total protein concentration increased after surgery (mean before surgery, 1.016 and 3.6 g/dl; mean after surgery, 1.021 and 5.6 g/dl).
Show more [+] Less [-]Clinicopathological observations on the experimental pancreatitis induced by ligation of pancreatic ducts
1994
Sung, E.J. | Lee, H.B. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine)
Enhancement of cell-mediated immunity by administration of plasma protein in pigs-(1)-Proportion of leukocyte subpopulations and cells expressing adhesion molecules in peripheral blood
1994
Yang, C.K. (Thaihan Industry Company, Seoul (Korea Republic). Veterinary Diagnostic Laboratory) | Kim, S.J. (Konkuk University, Seoul (Korea Republic). Department of Veterinary Medicine) | Moon, J.S. | Jung, S.C. | Park, Y.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Enhancement of cell-mediated immunity by administration of plasma protein in pigs-(2)-Proportion of T lymphocyte subpopulations and cells expressing MHC class 1, 2 molecules in peripheral blood
1994
Yang, C.K. (Thaihan Industry Company, Seoul (Korea Republic). Veterinary Diagnostic Laboratory) | Kim, S.J. (Konkuk University, Seoul (Korea Republic). Department of Veterinary Medicine) | Moon, J.S. | Jung, S.C. | Park, Y.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Effect of immunosuppression on Ascaris suum infection in undefinitive hosts-(3)-Investigations in mice
1994
Rhee, J.K. | Park, B.K. | Jang, B.G. | Yook, S.Y. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine)
Effects of administration of Lactobacillus acidophilus-fermented milk on hematological values and histopathological changes of kidney in cadmium-treated rats
1994
Cho, Y.C. (Chungnam National University, Taejon (Korea Republic). College of Medicine) | Jun, M.H. | Chang, K.S. (Chungnam National University, Taejon (Korea Republic). College of Veterinary Medicine)
Activity of myeloperoxidase and leukocyte peroxidase according to ages in the hen (Dekalbwarren)
1994
Chon, S.K. | Kang, C.W. | Lee, H.I. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine)
Effect of immunosuppression on Ascaris suum infection in undefinitive hosts-(2)-Investigations in golden hamsters
1994
Rhee, J.K. | Kim, H.C. | Park, B.K. | Lee, C.H. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine)