In the conditions of the Republic of Belarus the seasonal derangements of metabolic processes at high-producing cows usually take place in the period from March till May. Analysis of efficiency of application of national preparation CMP (complex-mineral preparation -iron (15 mg/ml); iodine (6,8 mg/ml); magnesium (6,0 mg/ml); selenium (0,32 mg/ml) and vitamin preparation Trivitim (vitamin A – 30000 IU; , vitamin D – 40000 IU; vitamin E – 20 mg) for the preserving of high milking yielding capacity and prevention of metabolic processes disorders was realized. Four groups of high productive cows were formed. First group was entered 10 ml of CMP intramuscular; cows of the second group were entered 10 ml of Trivitim intramuscular; cows of the third group were entered CMP and Trivitim in the same doses; and the fourth group was a controlled one. On the 1-st, 7-th and 15-th days there was realized evaluation animal clinical state, hematologic and biochemical blood indexes (leukocytes; erythrocytes; hemoglobin; thrombocytes; ESR; total protein; albumins; urea; creatinine; glucose; calcium; phosphorous; magnesium; iron), and average daily milk yield. There was also analyzed the phagocytic activity of neutrophilic leukocytes. Research results showed that content of leukocytes and ESR in blood in all experimental groups was within the standard limits. In the first experimental group there was state the increasing of hemoglobin (from 91,4 +/- 4,38 up to 111,83 +/- 4,3 g/l (P less than0,05) ) and erythrocytes (from 4,48 +/- 0,21 up to 5,97 +/- 0,45 10E12/l) content. The same changes were stated in cows of the 2-d and 3-d experimental groups. Content of hemoglobin and erythrocyte in blood of cows which were administrated with CMP and Trivitim increased from 90,6 +/- 0,06 to 99,83 +/- 3,61 g/l and from 4,51 +/- 0,27 to 5,55 +/- 0,28 10E12/l, respectively. Analyze of biochemical indexes of cow blood showed that content of total protein, calcium, phosphorus, magnesium and iron increased. On the 15-th day there was stated the decrease of urea. Research results proved that the combined application of CMP and Trivitim was efficient and prevented subclinical disorders of metabolic processes

Production examination of efficiency of concentrated vaccine against swine erysipelas from matrix Konev of trial series, control of its immunizing power and production experiments of biological preparation were realized in the conditions of the Republic of Belarus. Research results showed that the presented vaccine had more expressed immunogenic properties in comparison with its production analogue and provided piglets protection against infection. There was analyzed the immunological efficiency of the vaccine: determination of quantity of erythrocytes; leukocytes; content of hemoglobin, level of lysozyme activity, bacterial growth-inhibitory activity, blood serum, phagocytic activity, neutrophilic leukocytes, content of total protein and protein fractions, titre of erysipelas antibodies, and preventive properties of blood serum. Efficiency of biological preparation was determined on the basis of analysis of disease incidence rate, loss of immunized swine caused by swine erysipelas

Determination of productivity and natural resistance indexes of laying hens after application of dry multienzyme preparation Vitazim (xylanase, 3600 units/g; cellulose, 3000 units/g; beta-glucanase, 7000 units/g); as well as the specification of optimal doses the enzyme were realized in the conditions of the Republic of Belarus. Research results showed that the best indexes of egg yields of laying hens were obtained after application in the main poultry diet of enzyme preparation Vitazim in dose 500 g/t that made it possible to increase the productivity in the experimemtal group on 12%. Application of Vitazim also promoted the increasing of natural resistance level of laying hens. There was stated the positive effect of Vitazim on bactericidal activity of blood serum. Before the beginning of test this index was the level of 44,35+/-0,92 – 56,52 +/-1,63. By the 300-th day of life the bactericidal activity of blood serum in experimental group administrated Vitazim in dose 300 g/t exceeded the control idexes on 3,8%; in the second experimental group (Vitazim in dose of 500 g/h) – on 29,5%; in the third experimental group (/vitazim on dose of 700 g/h) – on 23,7%.Vitazim administration also exercised positive influence on lysozyme activity in blood serum of lying hens

Evaluation of a quantity and phagocyte activity of leucocytes, immunoglobulins, T- and B-lymphocytes in blood serum of sheep in the process of stimulation of central nervous system with caffeine sodiobenzoate for the increasing of nonspecific animal resistance was realized in the conditions of the Republic of Belarus. Romanov yeld ewes were divided into two groups. Experimental sheep were entered caffeine sodiobenzoate intramuscularly in dose 0,012 g/kg of live weight twice a day with 6 hours interval. Controlled group of sheep were entered physiological salt solution in accordance with the same scheme. Blood samples were taken before preparation entering and after 1, 3, 6, 12 and 24 hours of test beginning. Immunoglobulin concentration in blood serum was estimated by electrophoresis method in polyacrylamide gel. Quantity of T- and B- lymphocytes was estimated by active rosette test. Phagocyte activity of leucocytes was estimated with application of germ culture St. albus. Leucocytes number was accounted in Gorjaev's chamber. Research results showed that under conditions of central nervous system stimulation by caffeine sodiobenzoate there was stated the increasing of nonspecific resistance. It was proved by the increasing of the total number and phagocytic activity of leukocytes, immunoglobulins (IgG, IgA and IgM), T- and B- lymphocytes

Searching for new, effective and safe methods of treatment of hookworm disease (ancylostomatidosis) of dogs caused by Ancylostoma caninum and Uncinaria stenocephala was realized in the conditions of the Republic of Belarus on the basis of studying of spices composition and dissemination of Ankilostomatidae, age-specific and seasonal disease dynamics, testing of medical and preventive preparations Univerm and Fenbendazol. In the Republic of Belarus Ancylostoma caninum was stated 4,2% of studied cases and Uncinaria stenocephala – in 14,3%. The maximum intensity of the invasion was stated in summer and autumn periods: in cases of individual keeping – 19,4%; in cases of group keeping the disease transformed into enzootic form with 66,7% affect. In winter period there was the lowest invasion – up to 1,4%. The preparation Univerm administrated twofold with feed in dose of 0,1 mg/kg with 24 hours interval proved to be highly effective therapeutic agent with extensefficiency100%. Anthelmintic Fenbendazol administrated once with feed in dose of 0,005 g/kg proved to be highly effective with extensefficiency 80% and intensefficiency 98,84%. Hematological indexes of sick dogs showed the evidence of erythropenia (4,30-4,80 x 10E12/l), hemoglobinemia (113-119 g/l) and eosinophilia (up to 11%), as well as the increased quantity of leukocytes on 5,46% - 23,61 %, and the lowered quantity of lymphocytes on 15,84 % in comparison with healthy animals indexes